pick_open_reference_otus.py hangs on step 1

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Jun 21, 2016, 11:41:03 AM6/21/16
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I am having a problem with the pick_open_reference_otus.py command. This command has been working perfectly fine for me in the past, but with one of my datasets it hangs on step 1. My dataset consists of sequences from two projects that I combined using the cat command. When I run pick_open_reference_otus.py on each dataset separately it works, but on the combined set it will not. I see ocelots being called in my activity monitor and working at high CPU, but after that it drops down to nothing again. I attached all the log files I got. I tried to attach the zipped fna files, but I think the files are too big (any idea how I can upload them here?) The command I run is:

pick_open_reference_otus.py -i $PWD/seqs_Chick_Env -r /Users/kirstengrond/Desktop/KSU/Myproject/Analyses/QIIME/gg_13_8_otus/rep_set/97_otus.fasta -o $PWD/OpenRefOTUs -aO 3

Which is exactly what I used for the separate datasets, and for other, larger sets. I also manually added the two fna files together and have the same problem. I can't see anything odd in the fna files, and every samples has a different barcode (not that that should matter here), but they were on different Illumina runs. 

Has anyone had this same problem? Or is there anything wrong with how I combined the files?



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