Hi,
It looks like the scale is wrong or missing, or maybe ImageJ has set a
different scale? I have found several problems (with the older CASA
plugins). When I had set a scale in ImageJ, it interfered with the
transformation pixels->micrometers in the plugin. You just had to go to
Set scale and then clear the scale in ImageJ, and then it worked. The
scale must be correctly set in the plugin, of course.
This does not discard that there is a problem with the software
elsewhere, but much like a scale issue for me.
Best wishes,
Felipe
El 8/9/20 a las 13:17, Alex Urban escribió:
> if I lower the area of minimum cell siize to 0.1um/pixel I get one path,
> if that helps
> [image: path.JPG]
>
> Alex Urban schrieb am Dienstag, 8. September 2020 um 11:01:46 UTC+2:
>
>> Hello, I'm having the same problem, regardsless on what I'm doing I do not
>> get any tracks....even ith the demo files and the recommended settings
>> ....unfortunatelly I cannot setup a new user on this PC. Is there anything
>> else I could try to get it working
>> Thanks for your help and cheers
>> Alex
>>
>>
wald...@gmail.com schrieb am Mittwoch, 2. September 2020 um 17:17:53
>> UTC+2:
>>
>>> Thanks Jesús!
>>>
>>> Em quarta-feira, 2 de setembro de 2020 às 02:53:33 UTC-5,
>>>
jya...@unizar.es escreveu:
>>>
>>>> Dear Waldir,
>>>> Yes, the software works with jpg files. You can use the imagej to modify
>>>> the file format.
>>>> Regarding the morphometry module, you have to select each sperm
>>>> individually to obtain results, just like using other comercial CASA
>>>> systems.
>>>> Best regards,
>>>> Jesús Yániz
>>>>
>>>> *De: *Waldir Miron <
wald...@gmail.com>
>>>> *Asunto: **Re: Not tracking the sperm cells*
>>>> *Fecha: *1 de septiembre de 2020, 23:33:26 CEST
>>>> *Para: *OpenCASA Mailing List <
opencasa_m...@googlegroups.com>
>>>>
>>>> Hi,
>>>>
>>>> I have just created a new user on Windows 10 and the software seems to
>>>> be working fine there. Just two minor questions on the morphometry module:
>>>>
>>>> 1) Does it only accepts .jpg files?
>>>>
>>>> 2) I still cannot get the results from the morphomety module. It does
>>>> not seem to automatically create the new window with the results for all
>>>> the sperm cells in the pictures.
>>>>
>>>> Many thanks,
>>>>
>>>> Waldir
>>>>
>>>> Em terça-feira, 1 de setembro de 2020 às 15:48:51 UTC-5, Waldir Miron
>>>> escreveu:
>>>>
>>>>> Hi
>>>>>
>>>>> I am having the same problem in several modules of OpenCASA. I have a
>>>>> Windows 10 machine, and it cannot count any cells in the viability test
>>>>> (using the example files), neither in v.1.0 or 2.0. I tried in a different
>>>>> computer using Linux and the version 2.0, it does not seem to work there
>>>>> either. Finally, I was informed to try ImageJ v.1.49 in Windows, but in my
>>>>> personal computer with Windows 10 it is not working.
>>>>>
>>>>> What else could I try?
>>>>>
>>>>> Many thanks, Waldir
>>>> <
https://groups.google.com/d/msgid/opencasa_mailinglist/51063ebe-e9d8-4842-becb-a357bd45d9een%40googlegroups.com?utm_medium=email&utm_source=footer>
>>>> .
>>>>
>>>>
>>>>
>