nonspecific search

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Carlos Alberto Gueto Tettay

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Jan 29, 2021, 10:45:21 AM1/29/21
to MS Amanda

Hi again,

can we add a rule at enzymes.xml for setting non-specific search?

thanks,


Carlos

Viktoria Dorfer

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Jan 29, 2021, 11:42:51 AM1/29/21
to MS Amanda
Hi Carlos,

in enzymes.xml there is a "No-Enzyme" "enzyme" that can be used for that. It will cleave after every amino acid. 
Similarly, there is a "No-Cleavage" "enzyme" for usage where the peptides/proteins in the fasta will be used as provided.
In addition, you can specify a semi-specific search if you change the "specificity" attribute of the enzyme in "settings.xml"


Best regards,
Viktoria 

Carlos Alberto Gueto Tettay

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Feb 1, 2021, 9:01:30 AM2/1/21
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this is really helpful!


Carlos


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