Hello Paul,
Please see attached. This gene in question (FUS) has I think 15 exons. I am seeing 19. Also, is there a way to remove denovo exons, which I am not interested in?
There is also another example that I can show you. The gene maybe has 22 or 23 exons, I am seeing 50 or so (too many denovo exons?). I would like to show that one to you as well.
The gtf file for the attached file was processed as follows:
1. MM10 ensembl gtf file was downloaded.
2. The chromosome names were converted to UCSC format from ensembl format.
3. Then I ran the gtf2gff converter.
Thank you in advance,
Nirad