I used the PF2 results into iqtree, and it runs successfully, but I got an unreliable result compared to the results from CIPRES(ML-tree),
a clade has a very long branch, and contains ten species that belong to some different clades/taxon in real. The parematers as follows:
iqtree -s XXXX.phy -spp XXXX.nex -bb 1000
And I also tried another method——run every gene tree in iqtree used the command line: iqtree -s XXXX.phy -m MFP,
after that, I got the best fit model for every gene used BIC, and I write a NEXUS format file manually like this:
#nexus
begin sets;
charset part1 = 1 -1530;
charset part2 = 1531 -2112;
charset part3 = 2113 -3114;
.......
charpartition mine = TVMe+R3:part1 , K3Pu+F+R2:part2 .......
At last, I used :
iqtree -s XXXX.phy -spp XXXX.nex -m MFP -bb 1000
There are warnning messages:
WARNING: Too high (saturated) partition rates for proportional partition model!
Current log-likelihood at step 1: -192589.529
But actually, I used plant mitochondrial genes, so generally, all of the genes have a relative lower evolutioan rate.
I was very confused if I used a set of wrong parameters or if I had misunderstood these procedures.
I look forward to your reply.
Thanks.
Yunxia