Hi,
i've attached only several sequences from the dataset in question including log files generated for them on my computer with the parallel_align_seqs_pynast.py script. Here are the two commands that I've used for the alignment:
parallel_align_seqs_pynast.py -i ../otus_flx_denovo/rep_set/seqs_chim_filt_rep_set.fasta -o pynast_aligned_seqs/coreset -e 170 -p 50 -t /media/sf_data/projects2015/core_set_aligned.fasta.imputed.fasta -T --jobs_to_start 10
parallel_align_seqs_pynast.py -i ../otus_flx_denovo/rep_set/seqs_chim_filt_rep_set.fasta -o pynast_aligned_seqs/70 -e 170 -p 50 -t /media/sf_data/projects2014/gg_13_8_otus/rep_set_aligned/85_otus.fasta -T --jobs_to_start 10
As you can see I had to lower the percent identity parameter so that the reads pass thruogh and don't get filtered.
Thanks for the help.
Slave