Microfabrication of organs-on-a-chip protocol

[Sebastian Freeman]

Hello everyone,

I was wondering if there was anyone out there who has any familiarity with the "Microfabrication of organs-on-chip" protocol. I am particularly interested in how to make the porous PDMS membrane. The protocol details how to make SU-8 silicon masters for the microchannels, but does not go into detail about how to make the master for the microarray of pillars. I had tried many protocols, but none with any consistent success.  If there is anyone who has achieved this and is willing to help, I would be very appreciative.

[C.V.CHANDRA SEKHAR REDDY]

Hi sebastian.

have you tried this 
http://www.nature.com/nprot/journal/v8/n11/full/nprot.2013.137.html

other wise

prepare initial template using Lb technique and then try the procedure

do let me know if you find any other procedure

my mail id is veera...@gmail.com

regards

Reddy

[Sebastian Freeman]

Hello Reddy,

That is the protocol I am currently following. I later found out that they did not make the pillar microarray master wafer themselves out of SU-8, but had another company etch it for them out of silicon. I am currently pursuing that road. Thank you for the other paper. I believe it will be useful.

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[Max Roberts]

Hi,

I was wondering if you every made progress in finding a company which was able to create the pillar microarray master wafer?  I am currently following the protocol in hopes of creating the  lung-on-a-chip but have sofar been unable to create the porous membrane.  Cheers in advance for any help regarding this.

On Tuesday, June 10, 2014 at 1:37:04 PM UTC+1, Sebastian Freeman wrote: