Hello Anuja,
When you upload a file to the blobstore, in the /_ah/upload callback you can grab the BlobKey that was uploaded:
BlobstoreService blobstoreService = BlobstoreServiceFactory.getBlobstoreService();
Map<String, List<BlobKey>> blobs = blobstoreService.getUploads(request);
BlobKey blobKey = blobs.get("file").get(0);
You need that blobkey to access the file again. If you want to accecss the file later, you might want to store it somewhere in the datastore.
With the BlobKey you can retrieve a byte[] of the file to do whatever you want:
byte[] file = blobstoreService.fetchData(
blobKey, 0, BlobstoreService.MAX_BLOB_FETCH_SIZE-1);
Hopes that helps.
Good luck!