Looking for pGLO

[Koeng]

Hey guys I want to trade some of my vectors for pGLO since I really don't wanna pay 40-50 bucks for it and have to ship it to my school. 

What I have to trade-

Vectors-

pBeloBac11

pUC19

Carolina's pGreen

pVIB

Strains-

Halobacterium NRC-1

I also have a Halobacterium vector I am testing right now. It is biobrick compatible, chloramphenicol resistant, shuttle vector with E coli. I am still testing it, (gonna make a new batch today cause I found out that mutagenesis products are linear...) and it should be about a week before I have a bunch of it. Here is the plasmid it is "based" off of.

http://www.atcc.org/Products/All/77216.aspx

I pretty much used a viral origin of replication for the Halobacteria, pUC origin for E coli, and chloramphenicol resistance from pBeloBac11. I also added a synthetic RBS before the chloramphenicol gene so (hopefully) it can be expressed in halo bacteria. However, I kept the same promoter because I think that the sigma factor for E coli may also work a little in halobacteria, added that I put a RBS site to maximize production. If ya wanna try the vector, just get some halobacteria broth and agar from carolina - http://www.carolina.com/prepared-biological-media/halobacterium-sp-nrc-1-media/FAM_775950.pr?catId=&mCat=&sCat=&ssCat=&question=halobacteria+media

I will give ya some of the strain for free :)

-Koeng

[Dakota Hamill]

I'll check. I'm 100% sure I have pVIB, and 87.83% sure I have pGLO.
Sometimes I confuse pGREEEN with pGLO though. Won't know until Monday
though when I can get to the freezer.

[Dakota Hamill]

I have some that was given to me by someone on this list, though I
don't know how much is left. I tried a transformation and it failed,
though I've since been working with the fungus that contaminated the
plate which has lead to more neat experiments, sometimes science works
like that!

I'm going to try to do a transformation later in the week and mini
prep the plasmid so I'll see what I can come up with! If I can find
the volume and concentration I have left and there is enough for more
than 1 transformation I'll pm you and spot some on a filter disc.

[Keoni Gandall]

Ok thanks!

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[Keoni Gandall]

Thank you very very much for the offer, and sorry for the late response, but I actually went ahead and bought pGLO already. Thanks though!

On Jun 1, 2013, at 3:49 PM, Петр Охрименко <tig...@gmail.com> wrote:

Hello
I have a similar plasmid.
map in pdf
It is located in E. coli BL21D3
If you are interested write me at E-Mal.


воскресенье, 7 апреля 2013 г., 19:49:48 UTC+3 пользователь Koeng написал:

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<GfpMaps.pdf>