CaCl2 transfection buffer without PIPES?

[Conner Berthold]

I was planning to run some CaCl2 transfections this Friday with a group of High School Students but don't have any PIPES handy. What is its role and will it be alright without it? It will be used it within 24 hours of making it.

Original Recipe:

60mM CaCl2

15% Glycerol

10mM PIPES, pH7

Thanks,

Conner

[Avery louie]

I just use 50mM CaCl, ice cold.  You dont really need the other stuff.

--A

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[Avery louie]

the protocol is on bosslab.org if you are interested in the specifics of the protocol we use.

--A

[Conner Berthold]

Thank you!

-Conner

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