This Weekend: Project BlueGene Review

[Avery louie]

Learn about creating indigo using bacteria!

When: Sunday, March 10, 1:30 p.m.

Where: BOSSlab - 359R Summer Ave, Somerville MA

What: Learn about BOSSlab's synthetic biology project: BlueGene.  Everyone is welcome to attend and propose suggestions for the project. The gist of the project is building a recombinant plasmid and transforming it into e. coli to produce indigo!  If that even sounds vaguely interesting, please come- if you know someone who it would interest, please invite them!

If you are coming, please RSVP here so we know to expect you.  If you have any questions just shoot me an email!

--A

If you want more information on last weekend, check out bosslab.org for photos and videos

[Avery louie]

Reminder: design review is tomorrow! It will be from 130-230 pm.  Please come by!

--A

On Mar 5, 2013 10:25 AM, <rob...@gmail.com> wrote:

HOW LATE DOES THIS GO TO?

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[Mega]

Awesome!! 

Unfortunately, you know,  I'm stuck in Europe... 

Maybe you want to share the plasmid some day for a little fee, producing indigo sounds interesting. In the middle ages it was one of the most expensive pigments... Would be funt to try to paint some textile with it ;) 

[Avery louie]

I will be more than happy to google hangout the event.  Also, a video of the review will be online afterwards.

Once I am done, I do intend to distribute the plasmid.

--A

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[Andreas Sturm]

Cool! 

As I said, usually you need X-Gal to get a colour reaction... But therefore you need to get the substance, and it costs money, shipment, and you may not get it as a private person. This system is awesome. 

Is there a unique  internal restriction site btw. ?

[Avery louie]

If what you are wondering is if it can be used to generate a chromogenic vector, the answer is yes!  It was done by Hart et al. In about 1990.  I forget what they called the plasmid they made, but it seems to have been lost to time.

--A

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[Andreas Sturm]

Sonds awesome! 

That's a pity though... Why would you prefer a system where you would have to put X-Gal in the medium over one which doesn't need X-Gal?? 

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[Andreas Sturm]

Would you mind posting the link to the sequence? 

Would like to search for  unique restriction sites inside ;)

[Andreas Sturm]

found the paper

Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production.

http://www.ncbi.nlm.nih.gov/pubmed/1556550

pSLH8 pasmid

[Avery louie]

yup.  sequence also at the somewhat-neglected bosslab.org/bluegene

[Andreas Sturm]

Ah ok, thanks!!! 

[Andreas Sturm]

Ok, I see an X-bal unique restriction site, 

one for 

BglI

PstI

SmaI

SphI

SalI is not usable as it is twice in, so a restriction does no blue-white screening then....

Seems quite usable without further modification