Where to get primers cheaply

[Mega]

Hi everyone... 

I'm going to have my first primers synthesized, and now I wonder where I can have them synthesized as cheap as posible (while still having a good sequence match)...

It would be one 200-mer and one 60; 110 and 63 

Anyone experience what that would roughly cost when doing a fair deal? 

[Cory Tobin]

I get my primers from either IDT or Valuegene. Costs around 0.35USD per base.

Although I doubt you will find anyone who can make a 200base primer.
IDT and Valuegene max out at 100.

Why do you want to make a 200base primer? You usually only need like
25-30 bases for PCR.

-cory

[Andreas Sturm]

Thanks...

The promoter should be attached this way. Another PCR for the primer makes it more complicated...

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[Nathan McCorkle]

I ordered from sigma-aldrich last for $0.15 USD for sequences around 40 bp

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[Avery louie]

I use IDT.  Thats a long primer though- it might be cheaper to have the promoter synthesized.

--A

[SC]

For the 200-mer, I'm assuming you mean a double-stranded fragment you can use for homologous recombination - is that what you had in mind?  I don't think a 200-mer can be used as a PCR primer.

 

You can have such fragments synthesized by Genscript.

[Andreas Sturm]

Hi,

No, I meant as a primer for PCR, where promoter is already attached...

On Sun, Mar 31, 2013 at 8:57 PM, SC <stac...@yahoo.com> wrote:

For the 200-mer, I'm assuming you mean a double-stranded fragment you can use for homologous recombination - is that what you had in mind?  I don't think a 200-mer can be used as a PCR primer.

 

You can have such fragments synthesized by Genscript.

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[Koeng]

Why is your promoter so big... I mean like normally promoters are like 35 bps..

http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119

Are you getting a operator and a RBS too?  Also a recommendation from me is to preform mutagenesis if your strands are that big.

-Koeng

[Andreas Sturm]

Well, it's just that chloroplast promotors are thatn big. 

Even the shorter still functional versions are around 50 bp long. 

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/iK8vYvgRZSMJ.

[Andreas Sturm]

And of the strongest, Prrn there is no short version known...