Pretty interesting pictures in some of the articles. Amazing you can
take out adipose tissue, diggest it, spin it, and harvest a plethora
of cell types.
As you mentioned, it looks like only 5% of that final fraction is
adipose derived stem cells. I imagine it will be difficult to select
only the ADSC's from that fraction with low-budget equipment. A flow
cytometry machine would be able to do it. A very careful hand and a
tiny pulled glass syringe might under a microscope.
I've done 0 human cell line culture in my life but even the lab next
to us who has an ozonator, secure room, hoods, everything, often
complains of contamination of fungal spores etc. It's not easy!
You may just take the cell mixture found in the VSF and culture that
and see what cells tend to dominate in it. It may be the stem cells
do well in culture and out-grow other types. It may be they do not,
and further purification to a monoculture is needed. How you'd do
that, I have no idea but it's interesting.
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