Cheapest sequencing deals?

[scoc...@gmail.com]

How much do you guys and gals pay for DNA sequencing these days? I've been shopping around and found the lowest to be $3. Anyone know of a place that can beat that? I just need a routine way to verify clones. I'd be happy with 400bp/read. I got super lucky once and got a 1200bp read in a single run from GenScript with no fancy prep or quant.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

[SC]

Are you looking to end-sequence plates of clones, or just a few?

[Avery louie]

I get my sequencing done at a place called wyzer biosciences.  They are local, service is extremely fast and they are very flexible- I walk my samples over.  Its about $5 bucks for a single read, less in plates or in larger quantities.

--A

Shop somerville first, as they say.

On Dec 1, 2014 11:37 AM, "'SC' via DIYbio" <diy...@googlegroups.com> wrote:

Are you looking to end-sequence plates of clones, or just a few?

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[Cory Tobin]

Just under 5.

Sequencing companies are usually willing to negotiate. If you can
guarantee enough volume or submit 96 well plates instead of single
tubes or agree to only pay with check/PO (save them the credit card
fees) then you can usually get a discount. Although, 3 USD is pretty
cheap already. I'm not sure you'll be able to shave off any more.

One trick I've used before: if you expect 9 out of 10 clones to be
good and you're just trying to weed out the bad 10% you can pool
samples together. In the first 400 or 500 (where the signal is really
clean) it will be obvious in the trace where the pooled samples
disagree. Instead of getting a single large peak on one channel you'll
see two smaller peaks on two channels. Toss out the bad pools and
keep the samples from the good pools. This won't work if you're
expecting a lot of variation between clones.

-cory

[Matt Lawes]

Talk to Jeswin here ... he works at one of the sequencing service providers with a couple different platforms.....

>matt

Sent from my Verizon Wireless 4G LTE DROID

Cory Tobin <cory....@gmail.com> wrote:

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