RE: [DIYbio] Measuring plant voltages

[Sebastian Cocioba]

SpikerBox works very well with plant action potentials. If you google Ivan Henriques, you may find his recent art work on a plant controlled robot. It takes the signal moves the not in a random direction. Its a simple DIY box and its very fun to play with. Good luck!

Sent from my Windows Phone

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From: Carlos Castellanos
Sent: 8/1/2013 9:20 PM
To: diy...@googlegroups.com
Subject: [DIYbio] Measuring plant voltages

Hi gang,

I'm hoping there is someone here with some knowledge of plant biology. I would be very grateful for any advice, references, etc on the subject of measuring plant voltages and other properties (e.g. pH). I'm thinking a GSR circuit using a wheatstone bridge and op-amp will do but I would appreciate any schematics/diagrams, etc

I would also like to see discussion of parameters, variables, experiments most galvanically active plants, etc. I am particularly interested in measuring voltages and biochemistry in wetland/marshy areas.

Any suggestions concerning other (simple) techniques for real-time feedback from plants would be appreciated.

Thanks!
-Carlos

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[Nathan McCorkle]

I just learned of the crescograph recently:
http://en.wikipedia.org/wiki/Crescograph

It seems someone in the SF Bay area has made one:
http://www.ebdir.net/enlighten/equipment.html

and something here
http://sonoma-dspace.calstate.edu/handle/10211.1/1665

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-Nathan

[Brian Degger]

Love the crescograph.

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[Tim @ Backyard Brains]

Hi Carlos,

The SpikerBox is an AC amp, and can't record the slow potentials of plants (for example, the action potential of a Venus Fly Trap is ~ 1 second long).

But, you are in luck. You only need about 100-200 gain to amplify the voltages of plants such as fly traps and sensitive mamomas.

There is a book on "plant electrophysiology"

http://www.springer.com/life+sciences/plant+sciences/book/978-3-540-32717-2

That is a bit expensive but perhaps you can find it at your local University Library.

Cheers,

Tim

[Nathan McCorkle]

Tim,
since you're more familiar with the field in general, have you ever
heard of the crescograph? Is it worth looking into more, and do you
know if we've definitely superseded it with other technology?

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-Nathan

[Tim @ Backyard Brains]

Actually this is the first time I have heard of a crescograph, but it seems like a cousin of the "smoking drum" machine (also called Kymograph) that was used to record the first neural action potentials. These were all developed in an age where timing circuits and printers were not readily available. 

http://en.wikipedia.org/wiki/Kymograph

Measuring plant measurement down to 1/10,000 of an inch is an impressive achievement, but now it just seems you could do it with a high quality digital camera with the plant growing in front of a background with calibration marks, using openCV or something like that.

[Carlos Castellanos]

Thanks for the tips everyone. Once we have something, I'll share it with you here.

-Carlos

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