Are there kits or a collected source to make bioluminesnce bacteria that react to gases like methane

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Dar Tu

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Jan 20, 2016, 11:50:27 PM1/20/16
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Hi, I'm a design student and I'm looking at making bio luminescent bacteria that will react if exposed to certain gases. Are there any available kits that I could purchase to do this or will I have to source parts individually? Also how long do you think it would take to have a viable outcome? 3 months?

Warm regards,


Bryan Jones

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Jan 21, 2016, 12:15:31 AM1/21/16
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Do you have a specific mechanism or pathway in mind. I doubt there are any commercial kits for something like that. I think you'd be more likely to find a preconstructed strain that someone would sell or share with you. 

On Wed, Jan 20, 2016, 5:50 PM Dar Tu <darr...@gmail.com> wrote:
Hi, I'm a design student and I'm looking at making bio luminescent bacteria that will react if exposed to certain gases. Are there any available kits that I could purchase to do this or will I have to source parts individually? Also how long do you think it would take to have a viable outcome? 3 months?

Warm regards,


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Nathan McCorkle

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Jan 21, 2016, 12:29:13 AM1/21/16
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I would use google scholar (or a similar search engine) to start
searching for "facultative methanotroph" (i.e. an organism that can
optionally grow on methane, if it is present)... this should lead you
to other facultative "gasotroph" (a word I just made up, intending to
mean it can eat 'gas') organisms. Another term that might help would
be chemotroph.
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Yuriy

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Jan 21, 2016, 9:57:32 AM1/21/16
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What are your other gasses?

Dar Tu

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Jan 21, 2016, 6:53:37 PM1/21/16
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On Thursday, January 21, 2016 at 4:57:32 AM UTC-5, Yuriy wrote:
What are your other gasses?



On Wednesday, January 20, 2016 at 7:15:31 PM UTC-5, Bryan Jones wrote:
Do you have a specific mechanism or pathway in mind. I doubt there are any commercial kits for something like that. I think you'd be more likely to find a preconstructed strain that someone would sell or share with you. 


Hi. :) I'm basically proposing health monitoring devices that use synthetic organisms and one way is through breath testing. I don't particularly have gasses in mind, more so just I'd like to have a proof of concept in that breathing onto can create a glow that can be measured by a luminometer.

Nathan McCorkle

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Jan 21, 2016, 8:34:56 PM1/21/16
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On Thu, Jan 21, 2016 at 10:53 AM, Dar Tu <darr...@gmail.com> wrote:
> Hi. :) I'm basically proposing health monitoring devices that use synthetic
> organisms and one way is through breath testing. I don't particularly have
> gasses in mind, more so just I'd like to have a proof of concept in that
> breathing onto can create a glow that can be measured by a luminometer.

Well remember that the gasses will have to either be soluble in the
media (some kind of water most likely, unless you're dealing with
something like Pichia pastoris) and/or your cell needs to have a
surface receptor/pump for the gas molecule. If it isn't soluble, that
isn't a deal-breaker... but you will definitely need something on your
cell to grab onto the gas as you bubble it into the media.

-Nathan

Yuriy

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Jan 22, 2016, 8:40:03 AM1/22/16
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Absolutes. 

I am sure some equilibrium for solubility exists. There are bacteria that can even sense hydrogen gas, solubility of which is lower than that of CO2. 

Xabier Vázquez Campos

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Jan 27, 2016, 12:27:35 AM1/27/16
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You might be interested in this paper I saw on Twitter few days ago,

Electroporation-Based Genetic Manipulation In Type I Methanotrophs

http://www.ncbi.nlm.nih.gov/pubmed/26801578
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