Meat Speciation for Newbies

[Ewan]

Hi All,

A few people and I would like to do some meat speciation tests, primarily as a learning project for PCR. We don't know anything about primer design at all, though, and would appreciate any pointers at all to help us get started. We've got the machine, reagents, and electrophoresis figured out, but this looks like the hardest part right now. How do we get from here to a primer which will amplify sequence specific to, for instance, cows?

I fully expect we'll need to do quite a bit of reading and messing around, which is fine, but we just don't know where to start.

Thanks for any help,

Ewan

[Nathan McCorkle]

Authentication of species in meat products by genetic techniques
Fátima C. Lago
European Food Research and Technology
March 2011, Volume 232, Issue 3, pp 509-515
http://link.springer.com/article/10.1007%2Fs00217-010-1417-1?LI=true#page-1
http://libgen.org/scimag2/10.1007/s00217-010-1417-1.pdf

Identification of Meat Species by Polymerase Chain Reaction (PCR) Technique
Turk. J. Vet. Anim. Sci.
2007; 31(3): 159-163
TÜBITAK
O. Irfan ILHAK**, Ali ARSLAN
http://journals.tubitak.gov.tr/veterinary/issues/vet-07-31-3/vet-31-3-3-0601-30.pdf

Detection of Adulteration and Identification of Cat’s, Dog’s, Donkey’s
and Horse’s Meat Using Species-Specific PCR and PCR-RFLP Techniques
Australian Journal of Basic and Applied Sciences, 3(3): 1716-1719, 2009
Salah M. Abdel-Rahman, Mohamed A. El-Saadani Khallid M. Ashry and
Amany S. Haggag
http://www.ajbasweb.com/ajbas/2009/1716-1719.pdf

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--
-Nathan

[jarlemag]

Hi. I've  gathered some notes with assorted primer sequences and some references (including the one above) on OpenWetWare. Haven't tried any of the primers myself, but  have a look if you want: http://openwetware.org/wiki/User:Jarle_Pahr/Meat

- JP

[Ewan]

Thanks greatly to both of you, that is everything we need to get cracking, and more! Nathan: how did you find those articles? I didn't have any luck with Google and don't have journal access. Half of the battle seems to be search skills with PCR projects.

Ewan

[Nathan McCorkle]

Jarlemag posted them to this list on march 6 2013

;)

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