Cheaply freezing Wisdom tooth

[Mega [Andreas Stuermer]]

Hi everyone.

Anyone got an idea how to freeze Wisdom teeth cheaply? I asked at a in-vitro-fertilization bank and at the Red Cross. The first said "absolutely no" while the second one said "rather no" perhaps quick and dirty, and 100€ per year.

 

Isn't there a cheaper way? What if I use -20°C? The cells probably won't be functional after few months right?

 

Any input appreciated ;)

[Cathal (phone)]

If you can't extract cells for proper preservation in FBS/DMSO, snap-freeze in nitrogen and store at -80, I think.

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[Dakota Hamill]

Snap freeze in liquid nitrogen then Negative 80 maybe?  Or just plan your experiment and when you're ready bust out the pliers and rip out a new root sample.  That'd be pretty hardcore though.

I could picture a diy'er pointing to missing teeth saying this experiment failed, worked, worked, failed.   Maybe there is a business opportunity in frozen teeth, get on it!

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[Andreas Stuermer]

I have got no -80°C freezer and I don't think university will allow me to throw it into theirs :P

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[Dakota Hamill]

Well luckily your teeth aren't going anywhere for a while, unless you are having them removed soon.  Why exactly do you want to freeze the tooth anyway?  

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[Koeng]

https://www.transcriptic.com/pricing/

11 bucks a year for -80 freezing

[Andreas Stuermer]

>11 bucks a year for -80 freezing

Plus shipment cost to america 1000$ overnight on ice? :D

(just guesstimating)

 

Plus, will they be functional after such long delivery on ice? Somwhere I read, though, that they were still cultivable after 5 days.

 

 

>Why exactly do you want to freeze the tooth anyway? 

http://www.ncbi.nlm.nih.gov/pubmed/21417716

These Japanese researchers are conducting a human trial at the moment. They take pulp from a tooth, put it into a nectrotic tooth and after 14 days it is vital again (regrowth of blood vessels and nerves)

 

I would really hate throwing those valuable stem cells away.

 

 

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[David Murphy]

it'll probably be possible to culture cells from some living teeth more reliably than from a years-old frozen wisdom tooth. probably better to invest the money and effort in keeping your existing teeth in good shape. 

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[Andreas Stuermer]

> probably better to invest the money and effort in keeping your existing teeth in good shape.

 

If you get hit accidentally, the tooth may die off and there is nothing you can do about it.

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