Looking for an Illumina Wizard

[Cathal Garvey]

Hey all,
I have an unusual project for which I'm planning to use Illumina as a
backend. However, the use-case is a novel repurposing of the platform,
and I'd really love to thresh it out with someone off-list.

If you are, or know, someone with direct practical experience on an
Illumina MiSeq, I'd love to have a chat about factors like barcode
format and length, potential throughput, costings etcetera.

Thanks!
Cathal

--
Twitter: @onetruecathal, @formabiolabs
Phone: +353876363185
Blog: http://indiebiotech.com
miniLock.io: JjmYYngs7akLZUjkvFkuYdsZ3PyPHSZRBKNm6qTYKZfAM

[André Esteves]

http://ehsm.eu/index.html#IlluminaSequencer

I was at this conference, but the presentation was not taped in fear of copyright and patent attacks...

Sadly i don't remember the name of the presenter... Maybe you could contact the conference organizers and pass your request for contact...

Cheers,

Aife

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[Cathal Garvey]

Cool, would have loved to see that!
It's a bit irrelevant to my needs, right now however. The basics of how
illumina works are widely known, it's the practicalities of how *well*
it works in nichey use-cases like mine that matter.

I've been told that it's unusual to multiplex more than 96 samples, and
that a few hundred (say 300) is really pushing the machine to its
limits. I'd like to hear if there's a credible dissenting view here,
because I thought Illumina could easily multiplex hundreds or thousand
of samples with reasonable coverage?

[André Esteves]

From what i gathered the machine is just an high res digital camera and UV lights with serial control. It's the zipper molecules with fluorescent ends plus the glass substrates that do the work. From knowing with reactant you are applying and the resulting image of dots, you can by logical inference aided by software sequence in parallel lots of dna strands...

The sauce is in the reactants...

Cheers,

Aife

[Rob O'Callahan]

We regularly push 2k+ multiplexed samples on MiSeq and NextSeq (and previously, GAIIx). https://www.eurekagenomics.com/ws/testing_and_analysis/ceseq.html