Is BioBricks Free Genes Project still maintained and alive?
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Dorif
May 4, 2020, 3:32:17 AM5/4/20
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Last submission ( https://docs.google.com/spreadsheets/d/1j5Gc7KEfRlPCIaXMGjDhgQDfSOVx7tnbss9AksrHhzk/edit?usp=sharing ) is in year 2018. Majority of sequences listed in spreadsheet are unavailable. I've tried to submit a gene (adenovirus death protein, for malignat cell lysis), but received no reaction, just autoreply from bot.
So, is it still alive?
Thanks for help!
Koeng
May 4, 2020, 11:33:01 AM5/4/20
to DIYbio
It's still alive, just turns out handling incoming single requests takes way too much time. I pretty much only handle collections of materials now. Some of those can be had on stanford.freegenes.org (password: freegenes).
If you have a good collection of genes (>20 genes) we can definitely talk, though.
Dorif
Apr 21, 2022, 5:23:40 PM4/21/22
to DIYbio
By the way, are you working at stanford freegenes.org ? If yes: may you help me? I need pro forma invoice for my order (FreeGenes-1584). Our customs are getting mad when see things with zero price. So I need this invoice with any ridiculous value for these items just to receive them. The same will be actual for FreeGenes-1599.
Thanks in advance!
понедельник, 4 мая 2020 г. в 18:33:01 UTC+3, Koeng:
Koeng
Apr 22, 2022, 12:21:25 PM4/22/22
to DIYbio
Not anymore, unfortunately
Dan Kolis
Dec 27, 2022, 12:13:40 PM12/27/22
to DIYbio
Is this summary correct ?
" The aspiration of many people in bio sciences that electronics provides macro concepts useful for application of nucleotide sequences is essentially incorrect. "
" These notions, ( that simplify life processes ), cannot be excised from viable life systems, including chaperones, post translation mRNA edits, long standing methylization attachments, and literally hundreds of biological effects implemented in life as built by Mother nature, in everything alive. "
Finally: "The reused terminology and desire to make VLSI like IDE's, ---may be useful in development of synthetic biology, but it appears this is almost simply a dead end. "
Is this possibly correct ?
Regards,
" The aspiration of many people in bio sciences that electronics provides macro concepts useful for application of nucleotide sequences is essentially incorrect. "
" These notions, ( that simplify life processes ), cannot be excised from viable life systems, including chaperones, post translation mRNA edits, long standing methylization attachments, and literally hundreds of biological effects implemented in life as built by Mother nature, in everything alive. "
Finally: "The reused terminology and desire to make VLSI like IDE's, ---may be useful in development of synthetic biology, but it appears this is almost simply a dead end. "
Is this possibly correct ?
Regards,
Daniel B. Kolis
PS I saw paper saying the actual wet-lab attempts with BioBricks DB plopped into glassware had a success rate by the applicable goals of each project of around 2%. My belief system is that is not zero... But the notion itself needs a full scale downgrade from first principles. What replaces it is: MORE USUAL HARD WORK AND NEW IDEAS ENTIRELY.
what do you think ?
Daniel B. Kolis
what do you think ?
Daniel B. Kolis
Eric
Dec 27, 2022, 2:59:07 PM12/27/22
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molecular cloning in itself as a molecular biology technique is automatically regarded to some/most as 'biobricks' activity but its use far predates the synthetic biology movement
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Dan Kolis
Dec 27, 2022, 3:59:38 PM12/27/22
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I thought the biobricks 'story' is a uniform 'design' of promoters and so on, so gene expression of novel organisms includes adding protein expression with a cookie cutter uniform approach.
I'd guess this involves protocol so the urge to fulfilment is with a predefined approach; ex cut with a certain R.I. add this or that to detect which cells 'took', etc.
Not the major contribution of them, then ?
Isn't something akin to mail order mitosis, in in itself, barely worth a yawn ?
Daniel B. Kolis
Abizar Lakdawalla
Dec 27, 2022, 8:51:47 PM12/27/22
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are Dan Kolis answers from a random sentence generator?
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Jonathan Cline
Dec 28, 2022, 11:53:17 AM12/28/22
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Biology is nanotechnology using nanomachines; therefore it is possible to create CAD systems which create compatible and/or replacement nanomachines to either interface with existing biology, or to simply create a New Biology. The present limitation is that the design rules of the nanotechnology are not well understood or replicable, thus leading to a "design by trial & error approach" rather than a "design by known rules" approach, and that is the fundamental weakness in biology and biological engineering today. Today's methods, BioBricks or other, are tremendously overhyped and the "design by trial & error" methods have a failure rate of near 100% when scaled beyond the trivial.
--
The journalists universally fail to report these facts and unfortunately are never held accountable.
########################
Dan Kolis
Dec 28, 2022, 12:56:21 PM12/28/22
to DIYbio
It was said moment ago...:
" Today's methods, BioBricks or other, are tremendously overhyped and the "design by trial & error" methods have a failure rate of near 100% when scaled beyond the trivial. The journalists universally fail to report these facts and unfortunately are never held accountable. "
Dan says:
Regarding the blued text especially.
Huh. I suppose this is roughly a verification of what I believed and poked in here as text, unfortunately.
It's remarkable how ( hopefully its unintentional ) misleading the WWW stitched around databases is for these alleged magic rabbits-from-hats systems composed of WWW programs, fat PDF's and CSV ( or more fashionably, TSV ) files.
I worry I am rattling on about the obvious, but you might notice I am interested in numbers that create milestones in time, or other ways to estimate actual, as opposed to synthetic, progress.
One way I thought about it lately is the H.S.-tuna-fish-to-ATP benchmark. The baseline is, if I gobble back a tuna fish sandwich and a map is developed from plate to ( ATP and 10 A.A); and we assume the area under the curve is 95% the mass of the food; ( ex. exclude metabolics for the olives, maybe etc.). How many unique proteins are in that transformation ?
e1) < 100
Huh. I suppose this is roughly a verification of what I believed and poked in here as text, unfortunately.
It's remarkable how ( hopefully its unintentional ) misleading the WWW stitched around databases is for these alleged magic rabbits-from-hats systems composed of WWW programs, fat PDF's and CSV ( or more fashionably, TSV ) files.
I worry I am rattling on about the obvious, but you might notice I am interested in numbers that create milestones in time, or other ways to estimate actual, as opposed to synthetic, progress.
One way I thought about it lately is the H.S.-tuna-fish-to-ATP benchmark. The baseline is, if I gobble back a tuna fish sandwich and a map is developed from plate to ( ATP and 10 A.A); and we assume the area under the curve is 95% the mass of the food; ( ex. exclude metabolics for the olives, maybe etc.). How many unique proteins are in that transformation ?
e1) < 100
e2) > 100 < 1000
e3) > 1000 < 5000
If you go super low and dare e2. think how reliable each transformation has to be characterised to win ? If many are 30 xforms long ... and 98% percent grokked. 30^ in that subset is in for failure in series is ... 0.98^30 = 0.55
So if either the deviation from the central dogma, errors of omission, mini-fraud, whatever is at 98% some are only 1/2 good. out of 30 of those, the chance ones important and completely obliterates the analysis is kind of well, certain.
So if either the deviation from the central dogma, errors of omission, mini-fraud, whatever is at 98% some are only 1/2 good. out of 30 of those, the chance ones important and completely obliterates the analysis is kind of well, certain.
So beyond P.R. to get funded, get queen-kissing-ops with Nobel Prizes, etc Mother nature has made this not medium hard but really, really hard.
I wonder if this kind of thinking has utility, or the general approach is: "Shut up and keep shoveling", that is the hard way is the only way... period.
A.I. programs kind of allow some more deviation to allow the unknown, but expecting a lot of them seems to me more misrepresentation, maybe.
I'd suggest a lot more humility in goals, and specifically NAMING of projects etc would help slightly. To explicitly discuss hoping to get from 2% to 10%, not a vague sci-fi ready story that's some mix of wishful thinking and fraud.
I wonder if this kind of thinking has utility, or the general approach is: "Shut up and keep shoveling", that is the hard way is the only way... period.
A.I. programs kind of allow some more deviation to allow the unknown, but expecting a lot of them seems to me more misrepresentation, maybe.
I'd suggest a lot more humility in goals, and specifically NAMING of projects etc would help slightly. To explicitly discuss hoping to get from 2% to 10%, not a vague sci-fi ready story that's some mix of wishful thinking and fraud.
Regs,
Daniel B. Kolis
my ref: BioBricks, blog, 28 Dec 2022, JonC
Eric
Dec 29, 2022, 3:07:58 PM12/29/22
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as the saying goes 'biology is technology,' there's even a book with such a title
but technology as a term that engineers use should refer to 1) who built it, and 2) what it's built to be used for, nothing more. thus it seems we're caught between a technology without an application (think google glass) and something more sinister and disturbing, so i'd just leave it at the former
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Dan Kolis
Dec 29, 2022, 5:12:50 PM12/29/22
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Well, I'm more interested in making molecules listen up to human urges then worry endlessly about what those urges might be.
I mean, is it time to yawn on this line item yet ? Scholars heavy hitting on the invoicing-the-learned-institutions on systems for a perfect world go nowhere. Until the things do something all the way, no decisions are made on what's what. I think that sort of thing is for all practical purposes, a total waste of time.
But even a single line item in the full flower of the 'story of biotechnology' can be pretty remarkable. For instance, tweak crops so just the right amount of nitrogen is fixed back without fertilizer, or maybe cure a couple diseases here and there.
And this moves Biotech from Sciencey fun stories to the reliability of technology. And a DIY subset is a perfect straddle of Science as fun to technology, totally. So This is a good spot to discuss how to make things work I think first, since what they do is decided by crazy uniformed processes anyway, yowling about it is a waste of attention span.
Of course, invoicing 3rd parties to dispense wisdom nobody listens to much is great work if you can get it.
Daniel B. Kolis
Dan Kolis
Jan 19, 2023, 11:57:28 AM1/19/23
to DIYbio
The book "DIY Scientists Hack the Software of Life" is breezy and full of DIY stories not much technology or science really.
2011 Marcus Wohlsen
Well written from an entertainment point of view. but lots of the little side projects and people stories are interesting enough. I'd say worth a lib lend for sure. I happily Borrowed a paper copy from Toronto's bizarrely well equipt public library.
Lots of San Fran, NYC and Boston in this book of course.
2011 Marcus Wohlsen
Well written from an entertainment point of view. but lots of the little side projects and people stories are interesting enough. I'd say worth a lib lend for sure. I happily Borrowed a paper copy from Toronto's bizarrely well equipt public library.
Lots of San Fran, NYC and Boston in this book of course.
And so it goes,
Daniel B. Kolis
Dorif
Jul 3, 2025, 5:31:46 AMJul 3
to DIYbio
Hey, guys, what's happening with project site? It just redirects at some Turkish online game shop now. What the hell is going on there???
четверг, 19 января 2023 г. в 18:57:28 UTC+2, dank...@gmail.com:
Koeng
Jul 3, 2025, 10:38:08 AMJul 3
to DIYbio
it dead I guess
Keoni Gandall
Jul 3, 2025, 2:10:02 PMJul 3
to openbioscience, diy...@googlegroups.com
Yeah I still operate in this area. Took like 2-3 years off though to go cofound a different company in robotics, but back at it.
Nowadays I'm making some open source part toolkits from oligo pools (got a quite good oligo assembly method) myself. Synthesis is kinda cheap if you go to pools. These are my latest designs I designed over a couple days:
Roughly 175 parts, but definitely gonna be making more soon.
.
├── bsub
│ ├── BsubPars.md
│ ├── DS_amyE.md
│ ├── DS_glmS.md
│ ├── DS_lacA.md
│ ├── DS_sigF.md
│ ├── DS_thrC.md
│ ├── DS_yhgE.md
│ ├── DS_yrbD.md
│ ├── P3P.md
│ ├── P43.md
│ ├── P_J23101.md
│ ├── PlepA.md
│ ├── PliaG.md
│ ├── PliaI.md
│ ├── Pman_manR.md
│ ├── Pmtl-short.md
│ ├── Ptet_tetR.md
│ ├── Pveg.md
│ ├── PxylA.md
│ ├── PxylA_xylR.md
│ ├── TO_pE194ts.md
│ ├── TO_pHT315.md
│ ├── US_amyE.md
│ ├── US_glmS.md
│ ├── US_lacA.md
│ ├── US_sigF.md
│ ├── US_thrC.md
│ ├── US_yhgE.md
│ ├── US_yrbD.md
│ ├── camR-Bsub.md
│ ├── eryR-Bsub.md
│ ├── kanR-Bsub.md
│ ├── pAMbeta1.md
│ ├── pBM300.md
│ ├── pBS72.md
│ ├── pCG1.md
│ ├── pCM66.md
│ ├── pGRB1.md
│ ├── pIP404.md
│ ├── pLS20.md
│ ├── pMK3.md
│ ├── pMTLB.md
│ ├── pMflST.md
│ ├── pNG168.md
│ ├── pSK41.md
│ └── spcR-Bsub.md
├── kmax
│ ├── ARS1.md
│ ├── ISceI_Kmax.md
│ ├── OpenCRISPR_yeast.md
│ ├── PARS1.md
│ ├── PIN450v2_promoter.md
│ └── ntag_INU1.md
├── mtk
│ ├── MTK2_001.backup
│ ├── MTK2_003.backup
│ ├── MTK2_004.md
│ ├── MTK2_005.md
│ ├── MTK2_006.md
│ ├── MTK2_007.md
│ ├── MTK2_008.backup
│ ├── MTK2_009.md
│ ├── MTK2_012.md
│ ├── MTK2_013.md
│ ├── MTK2_014.backup
│ ├── MTK2_015.md
│ ├── MTK2_016.md
│ ├── MTK2_017.md
│ ├── MTK2_018.md
│ ├── MTK2_019.md
│ ├── MTK2_020.md
│ ├── MTK2_021.md
│ ├── MTK2_022.md
│ ├── MTK2_023.md
│ ├── MTK2_024.md
│ ├── MTK2_025.md
│ ├── MTK2_026.md
│ └── blank.md
├── other
│ └── green_smutans.md
├── pichia
│ ├── EGFP_Pp.md
│ ├── PARS.md
│ ├── Pichia_Bxb1.md
│ ├── RFP_Pp.md
│ ├── enzymes
│ │ ├── BbsIA_Pp.md
│ │ ├── BbsIB_Pp.md
│ │ ├── BsaI_Pp.md
│ │ ├── BtgZI_Pp.md
│ │ ├── Esp3I_Pp.md
│ │ ├── GFP11_Pp.md
│ │ ├── GFP1_10_Pp.md
│ │ ├── OpenTn5_Pp.md
│ │ ├── PNK_Pp.md
│ │ ├── PfuSso7d_Pp.md
│ │ ├── RKOD_Pp.md
│ │ ├── T5exo_Pp.md
│ │ ├── T7RNAp_Pp.md
│ │ ├── T7endoI_Pp.md
│ │ ├── TaqLigase_Pp.md
│ │ ├── TaqPolymerase_Pp.md
│ │ ├── phi29_Pp.md
│ │ └── phiBT1int_Pp.md
│ ├── ntag1_2
│ │ ├── CtUlp1_Ec.md
│ │ ├── ntag1_pichia_alphaAmylase_alphaMFDelta.md
│ │ ├── ntag1_pichia_alphaMF.md
│ │ ├── ntag1_pichia_alphaMFDelta.md
│ │ ├── ntag1_pichia_alphaMFDelta_noKex.md
│ │ ├── ntag1_pichia_alphaMF_noEAEA.md
│ │ ├── ntag1_pichia_glucoamylase_alphaMFDelta.md
│ │ ├── ntag1_pichia_inulinase_alphaMFDelta.md
│ │ ├── ntag1_pichia_invertase_alphaMFDelta.md
│ │ ├── ntag1_pichia_killer_alphaMFDelta.md
│ │ ├── ntag1_pichia_serumalbumin_alphaMFDelta.md
│ │ ├── ntag2_gfp11_cbm3_sumo.md
│ │ └── ntag2_mCherry_sumo.md
│ ├── ntag_pichia_alphaAmylase_alphaMFDelta.md
│ ├── ntag_pichia_alphaMF.md
│ ├── ntag_pichia_alphaMFDelta.md
│ ├── ntag_pichia_alphaMFDelta_noKex.md
│ ├── ntag_pichia_alphaMF_noEAEA.md
│ ├── ntag_pichia_glucoamylase_alphaMFDelta.md
│ ├── ntag_pichia_inulinase_alphaMFDelta.md
│ ├── ntag_pichia_invertase_alphaMFDelta.md
│ ├── ntag_pichia_killer_alphaMFDelta.md
│ ├── ntag_pichia_serumalbumin_alphaMFDelta.md
│ ├── pAOX1_promoter.md
│ ├── pENO1_promoter.md
│ ├── pTPI1_promoter.md
│ ├── tAOX1_terminator.md
│ └── tAOX1_terminator_ctag.md
└── yeast
├── 2micron.md
├── ALD6_promoter.md
├── CCW12_promoter.md
├── CEN_ARS.md
├── CUP1_promoter.md
├── GAL1_promoter.md
├── HHF1_promoter.md
├── HHF2_promoter.md
├── HIS3.md
├── HTB2_promoter.md
├── HygromycinR.md
├── KanamycinR.md
├── LEU2.md
├── MFA1_promoter.md
├── MFalpha2_promoter.md
├── NourseothricinR.md
├── PAB1_promoter.md
├── PGK1_promoter.md
├── POP6_promoter.md
├── PSP2_promoter.md
├── RAD27_promoter.md
├── RET2_promoter.md
├── REV1_promoter.md
├── RNR1_promoter.md
├── RNR2_promoter.md
├── RPL18B_promoter.md
├── SAC6_promoter.md
├── SceI_Scere.md
├── TDH3_promoter.md
├── TEF1_promoter.md
├── TEF2_promoter.md
├── URA3.md
├── Venus_Scere.md
├── Venus_Scere_GRAStag.md
├── ZeocinR.md
├── mRuby_Scere.md
├── mRuby_Scere_GRAStag.md
├── mTurquoise2_Scere.md
├── mTurquoise2_Scere_GRAStag.md
├── tADH1_terminator.md
├── tADH1_terminator_ctag.md
├── tENO1_terminator.md
├── tENO1_terminator_ctag.md
├── tENO2_terminator.md
├── tENO2_terminator_ctag.md
├── tPGK1_terminator.md
├── tPGK1_terminator_ctag.md
├── tSSA1_terminator.md
├── tSSA1_terminator_ctag.md
├── tTDH1_terminator.md
└── tTDH1_terminator_ctag.md
├── bsub
│ ├── BsubPars.md
│ ├── DS_amyE.md
│ ├── DS_glmS.md
│ ├── DS_lacA.md
│ ├── DS_sigF.md
│ ├── DS_thrC.md
│ ├── DS_yhgE.md
│ ├── DS_yrbD.md
│ ├── P3P.md
│ ├── P43.md
│ ├── P_J23101.md
│ ├── PlepA.md
│ ├── PliaG.md
│ ├── PliaI.md
│ ├── Pman_manR.md
│ ├── Pmtl-short.md
│ ├── Ptet_tetR.md
│ ├── Pveg.md
│ ├── PxylA.md
│ ├── PxylA_xylR.md
│ ├── TO_pE194ts.md
│ ├── TO_pHT315.md
│ ├── US_amyE.md
│ ├── US_glmS.md
│ ├── US_lacA.md
│ ├── US_sigF.md
│ ├── US_thrC.md
│ ├── US_yhgE.md
│ ├── US_yrbD.md
│ ├── camR-Bsub.md
│ ├── eryR-Bsub.md
│ ├── kanR-Bsub.md
│ ├── pAMbeta1.md
│ ├── pBM300.md
│ ├── pBS72.md
│ ├── pCG1.md
│ ├── pCM66.md
│ ├── pGRB1.md
│ ├── pIP404.md
│ ├── pLS20.md
│ ├── pMK3.md
│ ├── pMTLB.md
│ ├── pMflST.md
│ ├── pNG168.md
│ ├── pSK41.md
│ └── spcR-Bsub.md
├── kmax
│ ├── ARS1.md
│ ├── ISceI_Kmax.md
│ ├── OpenCRISPR_yeast.md
│ ├── PARS1.md
│ ├── PIN450v2_promoter.md
│ └── ntag_INU1.md
├── mtk
│ ├── MTK2_001.backup
│ ├── MTK2_003.backup
│ ├── MTK2_004.md
│ ├── MTK2_005.md
│ ├── MTK2_006.md
│ ├── MTK2_007.md
│ ├── MTK2_008.backup
│ ├── MTK2_009.md
│ ├── MTK2_012.md
│ ├── MTK2_013.md
│ ├── MTK2_014.backup
│ ├── MTK2_015.md
│ ├── MTK2_016.md
│ ├── MTK2_017.md
│ ├── MTK2_018.md
│ ├── MTK2_019.md
│ ├── MTK2_020.md
│ ├── MTK2_021.md
│ ├── MTK2_022.md
│ ├── MTK2_023.md
│ ├── MTK2_024.md
│ ├── MTK2_025.md
│ ├── MTK2_026.md
│ └── blank.md
├── other
│ └── green_smutans.md
├── pichia
│ ├── EGFP_Pp.md
│ ├── PARS.md
│ ├── Pichia_Bxb1.md
│ ├── RFP_Pp.md
│ ├── enzymes
│ │ ├── BbsIA_Pp.md
│ │ ├── BbsIB_Pp.md
│ │ ├── BsaI_Pp.md
│ │ ├── BtgZI_Pp.md
│ │ ├── Esp3I_Pp.md
│ │ ├── GFP11_Pp.md
│ │ ├── GFP1_10_Pp.md
│ │ ├── OpenTn5_Pp.md
│ │ ├── PNK_Pp.md
│ │ ├── PfuSso7d_Pp.md
│ │ ├── RKOD_Pp.md
│ │ ├── T5exo_Pp.md
│ │ ├── T7RNAp_Pp.md
│ │ ├── T7endoI_Pp.md
│ │ ├── TaqLigase_Pp.md
│ │ ├── TaqPolymerase_Pp.md
│ │ ├── phi29_Pp.md
│ │ └── phiBT1int_Pp.md
│ ├── ntag1_2
│ │ ├── CtUlp1_Ec.md
│ │ ├── ntag1_pichia_alphaAmylase_alphaMFDelta.md
│ │ ├── ntag1_pichia_alphaMF.md
│ │ ├── ntag1_pichia_alphaMFDelta.md
│ │ ├── ntag1_pichia_alphaMFDelta_noKex.md
│ │ ├── ntag1_pichia_alphaMF_noEAEA.md
│ │ ├── ntag1_pichia_glucoamylase_alphaMFDelta.md
│ │ ├── ntag1_pichia_inulinase_alphaMFDelta.md
│ │ ├── ntag1_pichia_invertase_alphaMFDelta.md
│ │ ├── ntag1_pichia_killer_alphaMFDelta.md
│ │ ├── ntag1_pichia_serumalbumin_alphaMFDelta.md
│ │ ├── ntag2_gfp11_cbm3_sumo.md
│ │ └── ntag2_mCherry_sumo.md
│ ├── ntag_pichia_alphaAmylase_alphaMFDelta.md
│ ├── ntag_pichia_alphaMF.md
│ ├── ntag_pichia_alphaMFDelta.md
│ ├── ntag_pichia_alphaMFDelta_noKex.md
│ ├── ntag_pichia_alphaMF_noEAEA.md
│ ├── ntag_pichia_glucoamylase_alphaMFDelta.md
│ ├── ntag_pichia_inulinase_alphaMFDelta.md
│ ├── ntag_pichia_invertase_alphaMFDelta.md
│ ├── ntag_pichia_killer_alphaMFDelta.md
│ ├── ntag_pichia_serumalbumin_alphaMFDelta.md
│ ├── pAOX1_promoter.md
│ ├── pENO1_promoter.md
│ ├── pTPI1_promoter.md
│ ├── tAOX1_terminator.md
│ └── tAOX1_terminator_ctag.md
└── yeast
├── 2micron.md
├── ALD6_promoter.md
├── CCW12_promoter.md
├── CEN_ARS.md
├── CUP1_promoter.md
├── GAL1_promoter.md
├── HHF1_promoter.md
├── HHF2_promoter.md
├── HIS3.md
├── HTB2_promoter.md
├── HygromycinR.md
├── KanamycinR.md
├── LEU2.md
├── MFA1_promoter.md
├── MFalpha2_promoter.md
├── NourseothricinR.md
├── PAB1_promoter.md
├── PGK1_promoter.md
├── POP6_promoter.md
├── PSP2_promoter.md
├── RAD27_promoter.md
├── RET2_promoter.md
├── REV1_promoter.md
├── RNR1_promoter.md
├── RNR2_promoter.md
├── RPL18B_promoter.md
├── SAC6_promoter.md
├── SceI_Scere.md
├── TDH3_promoter.md
├── TEF1_promoter.md
├── TEF2_promoter.md
├── URA3.md
├── Venus_Scere.md
├── Venus_Scere_GRAStag.md
├── ZeocinR.md
├── mRuby_Scere.md
├── mRuby_Scere_GRAStag.md
├── mTurquoise2_Scere.md
├── mTurquoise2_Scere_GRAStag.md
├── tADH1_terminator.md
├── tADH1_terminator_ctag.md
├── tENO1_terminator.md
├── tENO1_terminator_ctag.md
├── tENO2_terminator.md
├── tENO2_terminator_ctag.md
├── tPGK1_terminator.md
├── tPGK1_terminator_ctag.md
├── tSSA1_terminator.md
├── tSSA1_terminator_ctag.md
├── tTDH1_terminator.md
└── tTDH1_terminator_ctag.md
On Thu, Jul 3, 2025 at 12:48 PM openbioscience <openbio...@gmail.com> wrote:
Last time I checked there was a message that FreeGenes deliveries were on hold due to resource unavailability.
Pitty.Keoni Gandall carried the torch for a while but I'm not sure if he still operates in this area because I have not heard about his recent activities.
I have some of the constructs but I had some issues with the fridge so I'm not sure if they are still viable.
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Dan Kolis
Jul 3, 2025, 2:58:13 PMJul 3
to DIYbio
My perception ( could be considerably wrong ) was the base notion was not quite viable enough to have momentum.
things wrong:
1) Sticky versus wet ends meant lots of modules didn't really concatinate well.
2) Baseline back stove notion is "proteins are like flip flops", is just simply untrue enough descriptions of functions don't work most of the time
Those may be 'just two things wrong' but there big things.
Corrections or so on to poke in here is valuable. I mean the idea in principle is pretty yummy.
Regs,
Daniel B. Kolis
03 Jul 2025
things wrong:
1) Sticky versus wet ends meant lots of modules didn't really concatinate well.
2) Baseline back stove notion is "proteins are like flip flops", is just simply untrue enough descriptions of functions don't work most of the time
Those may be 'just two things wrong' but there big things.
Corrections or so on to poke in here is valuable. I mean the idea in principle is pretty yummy.
Regs,
Daniel B. Kolis
03 Jul 2025
Dakota Hamill
Jul 3, 2025, 5:49:16 PMJul 3
to diy...@googlegroups.com
Free Genes was/is a sweet idea. I still wish anyone could get an iGEM distribution kit. Still seems locked behind institutional access.
To view this discussion visit https://groups.google.com/d/msgid/diybio/61d03532-5339-4368-852f-4ae0a920d889n%40googlegroups.com.
Dan Kolis
Jul 4, 2025, 12:25:37 PMJul 4
to DIYbio
I would think all these sexy pseudo humanitarian projects would benefit from a post mortem when the evaporate into nothingness.
It's fairly likely there existence, though not sustained often has some non-trivial utility. When one flops back to "training for the young, etc" its fairly apparent this is a segway to not existing. Yet, that is: stand alone a worthwhile goal. IGEM is hugely in that camp.
Reality is messy, and so are a little string of molecules than enable life. Some somewhat similar organisational stuffs include:
Gavi, the Vaccine Alliance, the Coalition for Epidemic Preparedness Innovations (CEPI)
All these, even GHDDI ( Side project of Bill Gates, more or less ), have this science + goodwill glossy story, but when the reality kicks in, are hard to map-up, sustain, defend functionally. Not just pay for, but simply to do on an ongoing basis...
But the best way to do a needy thing if you get away with it, is start with a copy of something that works, that is documented, and complete. Often that is a the notion to wait HH:MM:SS for patents to expire and do it for any other purpose, for many of these sorts of endeavours. SO, you might think goodwill for the human experience for some i fit just says to hugely reduce the timeframe for patents. and frustrate rolling them over with mini-lies.
Big DUNNO at my end of the whole thing.
Id open source similiar, IE github ? Most of these snagged chucnks of code 'OH FOR FREE' are useless as-is almost, but many launch something that works significantly, benefits somebody, anyway. Or well, works and the head start matters.
Maybe the expectations at the receiving end are as much of a problem as the slightly underbaked stuffs in the offerings.
Daniel B. Kolis
04 Jul 2025
It's fairly likely there existence, though not sustained often has some non-trivial utility. When one flops back to "training for the young, etc" its fairly apparent this is a segway to not existing. Yet, that is: stand alone a worthwhile goal. IGEM is hugely in that camp.
Reality is messy, and so are a little string of molecules than enable life. Some somewhat similar organisational stuffs include:
Gavi, the Vaccine Alliance, the Coalition for Epidemic Preparedness Innovations (CEPI)
All these, even GHDDI ( Side project of Bill Gates, more or less ), have this science + goodwill glossy story, but when the reality kicks in, are hard to map-up, sustain, defend functionally. Not just pay for, but simply to do on an ongoing basis...
But the best way to do a needy thing if you get away with it, is start with a copy of something that works, that is documented, and complete. Often that is a the notion to wait HH:MM:SS for patents to expire and do it for any other purpose, for many of these sorts of endeavours. SO, you might think goodwill for the human experience for some i fit just says to hugely reduce the timeframe for patents. and frustrate rolling them over with mini-lies.
Big DUNNO at my end of the whole thing.
Id open source similiar, IE github ? Most of these snagged chucnks of code 'OH FOR FREE' are useless as-is almost, but many launch something that works significantly, benefits somebody, anyway. Or well, works and the head start matters.
Maybe the expectations at the receiving end are as much of a problem as the slightly underbaked stuffs in the offerings.
Daniel B. Kolis
04 Jul 2025
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