Hydroxocobalamin and cyanide

[Erico Perrella]

Hi everyone!

I am running some tests using hydroxocobalamin and cyanide, trying to build a low cost open source treatment for cyanide poisoning.

Do any of you know some easily obtainable bacteria that I could use for hydroxocobalamin synthesis?

Thank you!

Erico Perrella

[Erico Perrella]

Just found out that Propionibacteriom Shermanii is used in the industry for producing B12 vitamin!

Thanks!

2013/1/23 Erico Perrella <erico.p...@gmail.com>

[Nathan McCorkle]

Can you explain your test idea/protocol?

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[Dakota Hamill]

Are Vitamin B12 injections really that expensive? 

[Erico Perrella]

I am (together with my team) trying to create a device for mining waste treatment. 

Cyanide used on gold mining for example is still present on the waste after the beneficiation process. 

We have here in Brazil lots of areas contaminated with cyanide.

Hydroxocobalamin in contact with cyanide molecules usually forms cyanocobalamin (also B12 vitamin), so we were thinking about using it.

Here in Brazil the injections and the vitamin are quite expensive.

2013/1/26 Dakota Hamill <dko...@gmail.com>

Are Vitamin B12 injections really that expensive? 

--

[Dakota Hamill]

Very cool!  I didn't mean to come off as belittling the project, I was just naturally curios because it's not something you hear a lot about around here in New England aside from famous suicides or freak lab accidents.   It makes a lot of sense now in relation to the mining and leach fields.  So the cyanide binds to the Cobalt atom in the middle and becomes one of its axial ligands?

[Erico Perrella]

It seems so! We have just obtained the Propionibacteriom Shermanii from a friend and we are going to run some tests this week so if that is feasible.

2013/1/28 Dakota Hamill <dko...@gmail.com>

Very cool!  I didn't mean to come off as belittling the project, I was just naturally curios because it's not something you hear a lot about around here in New England aside from famous suicides or freak lab accidents.   It makes a lot of sense now in relation to the mining and leach fields.  So the cyanide binds to the Cobalt atom in the middle and becomes one of its axial ligands?

[John Griessen]

On 01/28/2013 03:00 PM, Erico Perrella wrote:
> We have here in Brazil lots of areas contaminated with cyanide.
> Hydroxocobalamin in contact with cyanide molecules usually forms cyanocobalamin (also B12 vitamin), so we were thinking about
> using it.
> Here in Brazil the injections and the vitamin are quite expensive.

This is another of Bucky Fuller's predictions happening. He described all
pollution as incomplete process reactants left lying around. They just need further
processing and then there's no pollutant.

NPR radio in the US just reported on the original 1849 California gold rush "mother lode"
Sutter mine operating in full swing again since shut down just before WWII because of sustained
high gold prices recently being enough to offset the risk of replacing the old flooded rotted
shoring in the narrow tunnels they mine gold in there. It's still high enough concentration
at the Sutter mine that they do not use solution mining methods, (cyanide).

[Erico Perrella]

Here in Brazil we have lots of amateur and illegal gold mines, and of course, in the most remote areas (inside the amazon forest for example), the regulators and the law do not reach.

Even the legal mines, until very recently had (by law) a "environmental flexibility" that allowed them to explore the resources without paying proper attention to what they were leaving behind, like trails of quicksilver, arsenic and cyanide.

2013/1/28 John Griessen <jo...@industromatic.com>

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[Josiah Zayner]

You know you can buy any and all the chemicals normally used for antidotes for really cheap, including B12?
Try eBay or online, I think they ship to Brazil....

On Tuesday, January 29, 2013 9:05:41 AM UTC-6, Erico Perrella wrote:

Here in Brazil we have lots of amateur and illegal gold mines, and of course, in the most remote areas (inside the amazon forest for example), the regulators and the law do not reach.

Even the legal mines, until very recently had (by law) a "environmental flexibility" that allowed them to explore the resources without paying proper attention to what they were leaving behind, like trails of quicksilver, arsenic and cyanide.

2013/1/28 John Griessen <jo...@industromatic.com>

On 01/28/2013 03:00 PM, Erico Perrella wrote:

We have here in Brazil lots of areas contaminated with cyanide.
Hydroxocobalamin in contact with cyanide molecules usually forms cyanocobalamin (also B12 vitamin), so we were thinking about
using it.
Here in Brazil the injections and the vitamin are quite expensive.

This is another of Bucky Fuller's predictions happening.  He described all
pollution as incomplete process reactants left lying around.  They just need further
processing and then there's no pollutant.

NPR radio in the US just reported on the original 1849 California gold rush "mother lode"
Sutter mine operating in full swing again since shut down just before WWII because of sustained
high gold prices recently being enough to offset the risk of replacing the old flooded rotted
shoring in the narrow tunnels they mine gold in there.  It's still high enough concentration
at the Sutter mine that they do not use solution mining methods, (cyanide).

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[Cathal Garvey]

I don't think he's suggesting making lots of B12 and pouring it on the
ground, rather I think the aim is to create a bioremediating strain of
bacteria that can be introduced to waste streams in order to maximise
impact with minimal input.

Am I right? :)

On 29/01/13 17:03, Josiah Zayner wrote:
> You know you can buy any and all the chemicals normally used for
> antidotes for really cheap, including B12?
> Try eBay or online, I think they ship to Brazil....
>
>
>
> On Tuesday, January 29, 2013 9:05:41 AM UTC-6, Erico Perrella wrote:
>
> Here in Brazil we have lots of amateur and illegal gold mines, and
> of course, in the most remote areas (inside the amazon forest for
> example), the regulators and the law do not reach.
> Even the legal mines, until very recently had (by law) a
> "environmental flexibility" that allowed them to explore the
> resources without paying proper attention to what they were leaving
> behind, like trails of quicksilver, arsenic and cyanide.
>
>

> 2013/1/28 John Griessen <jo...@industromatic.com <javascript:>>

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[Erico Perrella]

Yeah, exactly, we are creating a device that will actually process the waste.

2013/1/29 Cathal Garvey <cathal...@cathalgarvey.me>