Help required in sourcing cheap cell culture reagents

[Mary]

Hi All

I plan do do some basis cell culture but doent want to spend a fortune on the basis reagents needed to do this work and plan to source my ingredients from Health Food shops. I am looking for cheap and effective substitutes for the following:

1) L-Glutamine solution (can I make my own using health food glutamine?)

2) Penicillin/streptomycin (are there any natural alternatives?)

3) HEPES buffer

4) Hanks salt solution

5) Trypsin-EDTA solution used to subculture cells

6) Cell culture medium (can I make my own using health food supplies?)

All help is appreciated!

[Robert O'Callahan]

For 1: http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/media-supplements/GlutaMAX-Media/GlutaMAX-Media-Sample-Request-Form.html

-Rob

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Rob O'Callahan
M.S. Civil & Environmental Engineering 2012
George R. Brown School of Engineering | Rice University
E: rp...@rice.edu<mailto:rp...@rice.edu>| P: 847.641.1285

[Mega]

2) Penicillin/streptomycin (are there any natural alternatives?)

You could isolate that from a mould fungus, in theory.

Well, Alexander Flemming could do it many years ago ;)

[Steve]

Well, to be fair, he made little progress in extraction.  Howard Florey and Ernst Chain were the folks who eventually established an industrial extraction process.
Veterinary grade stock is probably your best bet.