Beginner Project, Trouble finding plasmids or culture.

[Chuckles]

So I'm a junior, general biology major and I thought it'd be fun to get my own little lab going. 

I want to do a simple little experiment to show to the nephews. I'd like to take some E. coli and make them glow by some good ol' fashioned bioluminescence using the pLux gene from Vibrio fischeri. I've done similar recombinant stuff in the lab at school and it's a super straight forward procedure so I thought it'd be fun. 

Trouble is... Where do I get the plasmids? I cannot find plasmids for the pLux gene anywhere. I thought about ordering a culture of V. fischeri and extracting the gene out via PCR... but I do not have a PCR machine yet. Hell, I don't even know if I can make a plasmid out of PCR products?? 

Thanks for the help! 

[Avery louie]

Carolina sells it.

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[Avery louie]

Additionally, you are the cool uncle.

On Jul 17, 2012 9:03 PM, "Chuckles" <charles...@gmail.com> wrote:

[Chuckles]

You know, I searched their site for "plux" but I didn't get anything. Just realized they autocorrected for "plug." 

Found it. 

Thanks!! 

[Mega]

You are lokking for the wrong name.

It's called pVIB. It's with an constitutive promotor, so no special medium required!

[Chuckles]

http://www.carolina.com/product/glow+in+the+dark+transformation+kit.do?keyword=vibrio&sortby=bestMatches

That would work, right?  

[Chuckles]

Correction

http://www.carolina.com/product/pvib+plasmid%2C+1+%26micro-g+%28200+%26micro-l-+0.005+%26micro-g-%26micro-l%29.do?keyword=pVIB&sortby=bestMatches

This! Sorry! 

[Andreas Sturm]

Yeah. I have that at home. You can even see them glowing at 10:00 nighttime when the window is open (weak light from streetlights and moon).

But you gonna need ampicillin for that one.

2012/7/18 Chuckles <charles...@gmail.com>

Correction

http://www.carolina.com/product/pvib+plasmid%2C+1+%26micro-g+%28200+%26micro-l-+0.005+%26micro-g-%26micro-l%29.do?keyword=pVIB&sortby=bestMatches

This! Sorry! 

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