Extremely cheap gene synthesis

[Mega]

Hi,


Some  days ago I was at a (I think it's called like this in english) symposium called living logic.

I met Gorge Church there and he mentioned that there are companies offereing new gene synthesis methods, one of them as cheap as  1000 Dollars for 6 Megabasepairs!! And he said the companies essentially do this on demand even today.

One of the companies mentioned was mycroarray. But on their homepage ther was no evidence that they did synthesis (of more than oglionucleotides). So I simply asked them via email, yet no answer...

Has anyone heard about that (gene synthesis cheap @ mycroarray) ??


 

[Nathan McCorkle]

You can take a DNA  microarray and wash off the oligos into a tube where they're all mixed together, then you can do "pooled assembly"

Not sure if mycroarray is a company it just another spelling of microarray.

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diy...@googlegroups.com.
To unsubscribe from this group, send email to diybio+un...@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/eVlwWP3JbGcJ.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

[Andreas Sturm]

http://www.mycroarray.com/

pooled assembly? Have to google that, but won't you need many restriction sites and enzymes to cut the oglios together?

[Nathan McCorkle]

here's a decent paper on the idea... IMO it's not next-gen enough for
me to really give a crap about, but it's a method to bridge to the
future for those who need DNA now

http://www.ncbi.nlm.nih.gov/pubmed/21113165

> --
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com.

> For more options, visit https://groups.google.com/groups/opt_out.
>
>

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Anselm Levskaya]

Hey all,

This industry is my bread and butter:

The cheapest "gene" synthesis that you can actually get on the market
costs $0.20/bp as linear 500bp fragments from IDT. It's not clonally
purified but is sequenced as a pool to eliminate pool-wide errors. On
a good day the net error rate is somewhere between 1/500 to 1/1000.
Turn-around time: ~1-2 weeks.

The cheapest clonally pure DNA is obtainable from a variety of chinese
companies (genscript, etc.) for $0.35/bp. It's not guaranteed they'll
actually be able to synthesize what you send them, and often they'll
fail, but they only charge on successful synthesis. Turnaround time:
4-6 weeks.

DNA2.0 (california) offers the strongest guarantees of turnaround time
at ~5-7days. However the cost for the service is upwards of $0.80/bp.
They're pretty good at making most things though.

No company has yet delivered to the public marketplace a next-gen
synthesis product with acceptable error rates. Gen9 and Cambrian
Genomics (my company) are the only startups that are even tackling the
problem. Calling it a solved problem is hyperbolic at this point,
though I hope that some real progress will be made over the next year
or two in bringing a solution to the general market.

(The problem with microarray pool amplification is that you have to
make oligos at such a high redundancy level on the array that the
per-oligo pricing starts looking a lot less competitive to
high-throughput bulk phosphoramidite synthesis in plates.)

-A