Complete beginner - where to start?

[Mega]

Are you familiar with the knowledge of plasmids. if not, read bout them first. they are like usb sticks of bacteria.

[Nathan McCorkle]

the 'central dogma' of molecular biology is pretty important

On Fri, Aug 3, 2012 at 3:43 AM, Mega <masters...@gmail.com> wrote:
> Are you familiar with the knowledge of plasmids. if not, read bout them first. they are like usb sticks of bacteria.
>

> --
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to diybio+un...@googlegroups.com.
> To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/aDtD8QBsJ68J.
> For more options, visit https://groups.google.com/groups/opt_out.
>
>



--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[JD]

I too would be very interested in knowing the answer to logicwin's question. I am in the same boat as he.

On Friday, August 3, 2012 10:50:06 AM UTC-4, logicwins wrote:

Is there a simple kit to order and start with or a book that isn't a textbook?  I have a background in finance and know that I will never become the best molecular biologist, just very interested in learning the basics and maybe doing a few experiments.  I learn better in a "lab" than just reading something out of a textbook.  I don't mind buying a few tools to start my own lab, just curious what I can do!

Thank you!

On Thursday, August 2, 2012 1:37:19 PM UTC-5, logicwins wrote:

I am really interested in learning about this stuff and I wanted to know if there was a good step by step log of how to do a simple first experiment and the tools necessary - to maybe make something that glows.  

Also, is there a particular book, pdf, wiki, or something else that summarizes things for someone who is not native to biology without having to restart college all over again?

Any other advice or help would be awesome.  

Thanks!

Justin

On Friday, August 3, 2012 10:50:06 AM UTC-4, logicwins wrote:

Is there a simple kit to order and start with or a book that isn't a textbook?  I have a background in finance and know that I will never become the best molecular biologist, just very interested in learning the basics and maybe doing a few experiments.  I learn better in a "lab" than just reading something out of a textbook.  I don't mind buying a few tools to start my own lab, just curious what I can do!

Thank you!

On Thursday, August 2, 2012 1:37:19 PM UTC-5, logicwins wrote:

I am really interested in learning about this stuff and I wanted to know if there was a good step by step log of how to do a simple first experiment and the tools necessary - to maybe make something that glows.  

Also, is there a particular book, pdf, wiki, or something else that summarizes things for someone who is not native to biology without having to restart college all over again?

Any other advice or help would be awesome.  

Thanks!

Justin

On Friday, August 3, 2012 10:50:06 AM UTC-4, logicwins wrote:

Is there a simple kit to order and start with or a book that isn't a textbook?  I have a background in finance and know that I will never become the best molecular biologist, just very interested in learning the basics and maybe doing a few experiments.  I learn better in a "lab" than just reading something out of a textbook.  I don't mind buying a few tools to start my own lab, just curious what I can do!

Thank you!

On Thursday, August 2, 2012 1:37:19 PM UTC-5, logicwins wrote:

I am really interested in learning about this stuff and I wanted to know if there was a good step by step log of how to do a simple first experiment and the tools necessary - to maybe make something that glows.  

Also, is there a particular book, pdf, wiki, or something else that summarizes things for someone who is not native to biology without having to restart college all over again?

Any other advice or help would be awesome.  

Thanks!

Justin

[Katherine Gordon]

Hello logicwins,

I am also very interested in a starting a "home lab" and would like to buy just the basics to begin with. If you get this information will you pass it on to me please? Thank you. Might I ask you what your goals are?

Kate

On Fri, Aug 3, 2012 at 8:50 AM, logicwins <logi...@gmail.com> wrote:

Is there a simple kit to order and start with or a book that isn't a textbook?  I have a background in finance and know that I will never become the best molecular biologist, just very interested in learning the basics and maybe doing a few experiments.  I learn better in a "lab" than just reading something out of a textbook.  I don't mind buying a few tools to start my own lab, just curious what I can do!

Thank you!

On Thursday, August 2, 2012 1:37:19 PM UTC-5, logicwins wrote:

I am really interested in learning about this stuff and I wanted to know if there was a good step by step log of how to do a simple first experiment and the tools necessary - to maybe make something that glows.  

Also, is there a particular book, pdf, wiki, or something else that summarizes things for someone who is not native to biology without having to restart college all over again?

Any other advice or help would be awesome.  

Thanks!

Justin

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diy...@googlegroups.com.
To unsubscribe from this group, send email to diybio+un...@googlegroups.com.

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/r_MLcnQ4npAJ.

[Nathan McCorkle]

They sell to anyone with a non-residential address. Just get it mailed
to you at work or to you c/o your nearest friendly business.

On Fri, Aug 3, 2012 at 4:09 PM, Alessandro Siletto
<alessandr...@gmail.com> wrote:
> hello, i've seen this kit that seems simple and for newbies. making e.coli
> glow with gene from a luminescent bacterium, using plasmid to transfer
> genes. cheap too.
>
> sadly they sell only to school or business :(
>
> http://www.carolina.com/product/life+science/genetics/special+genetics+topics/glow+in+the+dark+transformation+kit.do
>
> anybody know something similar that private can buy?

>
> --
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com.
> To view this discussion on the web visit

> https://groups.google.com/d/msg/diybio/-/hRGOJxDfJMoJ.

>
> For more options, visit https://groups.google.com/groups/opt_out.
>
>

[Mega]

I got only the plasmids of the kit from Carolina (they sell those to residental adresses!)

They are definitely worth the money (9$ !!)


Nathan brought a good idea how to get the entire kit.

But you said you would have some financial background and would also be willing to spend some $ on it?

So you could also buy the components,


Micropipettes 10 - 100 microliter (for bacteria, LB medium)  and 0.5 - 10 uL (for the plasmids) and some sterile tips
A pressure cooker
Calcium chloride or Polyethylen glycole
E. Coli (get it from a university or shared by someone
LB-Ampicillin petri dishes (pre-poured is far easier for beginners) and nutient agar dishes (e.g. lb agar) for starter culture
Some 1.5 ml Eppendorf tubes (microcentrifuge tubes) 
An incubator (on ebay maybe as low as 40$, but you'll surely get one for 120$ if you want to spend that much)

[Mega]

http://diyspartanbiotech.wordpress.com/category/genetic-engineering/

Here, at the bottom of the page you can see some photographes how the glowing bacteria will look like. Those were fresh transformed plates (I think it was in March or April),  a week ago or so I plated them (from the fridge) to fresh ampicillin-medium and they were glowing very very bright. You didn't even have to adapt your eyes to the darknes, just go into that room (without the carpets closed, the streetlights shining from outside) and see them glowing strongly!!

It truely was amazing!


(If you have 3000-3500 Dollars to spend we could even (try to) do this to a plant  :D )