BlueGene Project

[Avery louie]

Some of you at the meeting saturday heard me talk about the bluegene project, basically we want to take a gene from one bacteria and transfer it to another bacteria.  This gene codes for an enzyme that breaks down indole, and turns it into indigo.  Right now, the team is Cat, Simon, and myself.If you want to join, please do so this week, by Friday.  The cost of the project is basically whatever we buy, divided between all the people in the project.  I estimate that if nothing goes wrong, cost will be under $200 per person.

Project page can be found here: www.bosslab.org/BlueGene

--A

[Nathan McCorkle]

the page on bluegene: page not found

I'm interested to learn more of the enzyme system!

> --
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com.
> For more options, visit this group at
> http://groups.google.com/group/diybio?hl=en.



--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Avery louie]

http://bosslab.tumblr.com/

Everything should be live here now, for the time being.

--A

[Nathan McCorkle]

how are you planning to test for production of >2 cis-indole-2,3-dihydrodiol
??

[Nathan McCorkle]

oh i didn't look at the metacyc link to see the spontaneous steps to indigo :P

[Mega]

Hey,

The bacteria produces the pigment from scratch (glucose)? No need for external substrate??

[Avery louie]

Well, in that case, glucose would be the substrate, wouldn't it? :p

The indigo is produced by breaking down indole, which e. Coli naturally makes.

--A

On Jul 3, 2012 3:05 PM, "Mega" <masters...@gmail.com> wrote:

Hey,

The bacteria produces the pigment from scratch (glucose)? No need for external substrate??

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/RgT8GH9mk1QJ.

[Mega]

Surely, you're right. but e.g. the plasmid that makes e.coli smell like vanilla needs ferulic acid as a substrate. Who the hell has ferulic acid in his kitchen? ;D

[Avery louie]

As far as I know, you can just grow them on LB, but yes sometimes you need to add chemicals to get weird smells/colors.

--A

On Wed, Jul 4, 2012 at 8:50 AM, Mega <masters...@gmail.com> wrote:

Surely, you're right.   but e.g. the plasmid that makes e.coli smell like vanilla needs ferulic acid as a substrate.  Who the hell has ferulic acid in his kitchen? ;D

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/4J7vIsaWveoJ.

[Ethan]

As someone who is interested in molecular biology and organic chemistry (and with a bit of background in dye chemistry), I think this is a really cool project. Unfortunately, I am nowhere near you guys, nor do I have the money to put into this. I am excited to see how this goes. Keep us updated!

On Wednesday, July 4, 2012 10:47:02 AM UTC-4, Avery wrote:

As far as I know, you can just grow them on LB, but yes sometimes you need to add chemicals to get weird smells/colors.

--A

On Wed, Jul 4, 2012 at 8:50 AM, Mega <masters...@gmail.com> wrote:

Surely, you're right.   but e.g. the plasmid that makes e.coli smell like vanilla needs ferulic acid as a substrate.  Who the hell has ferulic acid in his kitchen? ;D

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/4J7vIsaWveoJ.

To post to this group, send email to diy...@googlegroups.com.

To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.

[Mega]

Indole is produced when digesting tryphtophane...
So I assume, all bacteria and animals have indole? Plants?


So you could also make a blue-skinned dog by attaching an animal promotor etc.  :D

[Nathan McCorkle]

On Mon, Jul 9, 2012 at 4:44 PM, Mega <masters...@gmail.com> wrote:
> Indole is produced when digesting tryphtophane...

or anabolizing tryptophan

> So I assume, all bacteria and animals have indole? Plants?
>
>
> So you could also make a blue-skinned dog by attaching an animal promotor
> etc. :D

Except the promoter must not be too strong or the animal could become
deficient in tryptophan... also maybe the dye has some toxicity/effect
in multicellular organisms

[John Griessen]

On 07/09/2012 03:44 PM, Mega wrote:
> So you could also make a blue-skinned dog

It's the blue hair that girls want...

[Andreas Sturm]

You should give that a try  :D

I'll buy a blue dog ;)