Biocurious came up with something. It involved running water through fertilizer, and then filter sterilizing, I think.
They can also be grown in regular sterilized seawater.
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Weird, I didn't know thongs could be deleted...
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Hi Loïc,
We've actually been doing a bunch of growth experiments with P. lunula at BioCurious lately. We handed out around 250 vials of dinoflagellates at the Bay Area Maker Faire a couple of months ago, along with a link to the following page of instructions:
http://tinyurl.com/MyPetDinos (I'll be updating that one soon with our latest results)
The official growth medium (L1 or f/2) is mostly nitrate by weight, so we figured we would give plant fertilizers a try. Turns out they grow on pretty much anything we've tried, provided you get the concentration in the right neighborhood. Higher concentrations of plant fertilizer do tend to cause more problems with cyanobacterial contamination, presumably because of the higher phosphorus levels than are found in the official medium. We've been getting our salt water from a local aquarium store, but you may be able to avoid this problem by sterilizing the water, or mixing it yourself from filtered water plus sea salts.
What size of "massive" growing do you have in mind here? Plant fertilizers are great because you can tell any ten year old how to grow dinoflagellates. But if you're really planning to grow massive amounts in a more controlled environment, you may want to have a look at the "Micro Algae Grow Mass Pack" here:
http://florida-aqua-farms.com/secure/agora.cgi?cart_id=3307010.22134*Ua7qr2&product=MICRO_MACRO_NUTRIENTS
One kg of the dry nutrients only costs $20 plus shipping, and is enough to make 10,000 liters! At that level, it's going to cost you more to filter or sterilize that much salt water...
On Monday, August 27, 2012 2:16:11 AM UTC-7, Loïc wrote:
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Seawater + garden plant fertilizer?
Mega why do you want to grow them in less salty water. Can get contamination with potentially bad bacterias.
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Does anyone know whether you can adapt the dinoflagellates (P. Fusiformis) to lower salt concentration / freshwater?
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I'm not up to date on what messages religious groups are pushing these days, but i'be heard Catholics have embraced evolution as a god-based mechanism. I guess its good to have some more people hopping on board whether they say physics is behind it or god.
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