BioCurious BioPrinter Instructable just published!

[Patrik D'haeseleer]

Come check out our brand-spanking new Instructable, on the BioPrinter Community Project at BioCurious:

http://www.instructables.com/id/DIY-BioPrinter/

We tried to document the various steps we took along the way as much as possible. We didn't include any hardware schematics, because the dimensions will depend on the specific CD drives you scavenge, and some of the rest is just "attach round peg A to square hole B with Shapelock and screws".

I think the project is easily replicable though, and could be made for less than $150.

Feel free to repost and vote for our Instructable...

Enjoy!

Patrik

[Cathal Garvey (Phone)]

Awesome!! Can ye put the "source" somewhere more open like gitorious or your wiki, so we can all see the images without instructables accounts?

Thanks to you/ye all!

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Sent from my Android phone with K-9 Mail. Please excuse my brevity.

[Sebastian S. Cocioba]

Oh thats just too awesome! Did you guys ever think of doing hyaluronic acid scaffolding experiments? Printing mammalian tissue? This has so much potential! 

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

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[Patrik D'haeseleer]

On Tuesday, January 22, 2013 12:37:47 PM UTC-8, Cathal Garvey (Phone) wrote:

Awesome!! Can ye put the "source" somewhere more open like gitorious or your wiki, so we can all see the images without instructables accounts?

I believe you should be able to see all the images regardless - I can see them without being logged in.

Is anyone else having trouble seeing the images?

Patrik

[Patrik D'haeseleer]

On Tuesday, January 22, 2013 12:39:11 PM UTC-8, Sebastian wrote:

Oh thats just too awesome! Did you guys ever think of doing hyaluronic acid scaffolding experiments? Printing mammalian tissue? This has so much potential! 

Printing scaffolding material is definitely on the list of things to do. I don't think we'll be working with animal cells for now, because they require a lot of maintenance, and that just doesn't work well with a community lab like BioCurious, where most people are only there a few days a week. Plant cells are much easier though!

Mainly, we're hoping to bring some fresh blood into the group, so we have some more hands to work on all the cool things you could do with this technology!

Patrik

[Bryan Bishop]

On Tue, Jan 22, 2013 at 2:54 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:

I believe you should be able to see all the images regardless - I can see them without being logged in.

I concur with Cathal. Would you guys consider using something like a version control system, so that other contributors can update the project or fork it?

- Bryan
http://heybryan.org/
1 512 203 0507

[Sebastian S. Cocioba]

I wonder if one could print protoplasts   onto MS-agar so when the cell walls form the walls may link? Plant based scaffolding... I gotta hit the lignin biosynth books to see if this is possible. I know as each protoplast divides, it forms colonies but iirc the mechanism for keeping each protoplast in contact with its daughter cells prior to cell wall formation is unknown. There are just too many fun things to do...this is all so overwhelming...yet awesome. Again, great job guys!

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

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[Nathan McCorkle]

For the InkShield microscope printhead pics, how do you know the dot
is the valve/pore and not on of the the darker rectangles?

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[Avery louie]

This is awesome!  I just spammed my maker friends with it.

--A

[Cathal Garvey]

I hadn't actually looked when I asked for it to be hosted everywhere,
it's just that Instructables as a platform is generally very naggy, and
prevents non-payers from doing simple things like zooming in, etc.

As Bryan points out though, revisions or improvements to the designs or
instructions could be suggested and implemented more fluidly using a
wiki or gitorious (the former is more user friendly, ofc.).

[Dakota Hamill]

Insanely awesome project.  Congrats on the glowing personalized E.coli text.  Hopefully that guy will start making more InkShield kits, he's all out.

[Patrik D'haeseleer]

On Tuesday, January 22, 2013 1:48:47 PM UTC-8, Nathan McCorkle wrote:

For the InkShield microscope printhead pics, how do you know the dot
is the valve/pore and not on of the the darker rectangles?

We don't truly know that the round opening is the nozzle, but everything we've seen and read on inkjet print heads so far leads us to believe that inkjet nozzles are round.

To be honest, we're waiting for one of our cartridges to clog up, so we can take it apart and study under the microscope, like we did with the other cartridge. For that matter, we're also not 100% certain that this cartridge doesn't have a filter built into the print head, like we found in the other one.

[Patrik D'haeseleer]

I have some personal attachment to Instructables, since I've been a supporter from back in the day they were just a bunch of guys in the Alameda control tower posting about dumpster diving and their kite surfing hardware. :-D Would it help non-members, at least in terms of accessibility, if I turned the whole thing into a pdf and made that available?

I would definitely not be opposed to reposting some of this where it can be more easily elaborated on by the community. We wrote the instructable essentially as a permanent record of what we've done - kinda like a journal article, but with a bit more of a story telling tone. I think that is a format that Instructables is very well suited for, and we'll probably keep on using it for that purpose unless we get enough pushback. But I don't feel it's really in a form that is suitable for rehashing. Maybe we could extract a more technical summary, and post that somewhere? More of a "How to build your own" version, rather than a "Here's all the things we did to get to this point"?

I've always been a big fan of wiki's, but I haven't really seen them being very effective for something like this. No experience with Gitorious. Any other suggestions?

To be honest, my main priorities right now are to make some progress on other projects, and write up a few more that we've had in the works, rather than reformatting something that is already online. Not to be contrarian, but I do have a day job and I need to prioritize my "ample spare time"...

Patrik

[Bryan Bishop]

On Tue, Jan 22, 2013 at 6:45 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:
> I've always been a big fan of wiki's, but I haven't really seen them being
> very effective for something like this. No experience with Gitorious. Any
> other suggestions?

How do you feel about how the reprap project core contributors
contribute together?

[Patrik D'haeseleer]

On Tuesday, January 22, 2013 4:53:08 PM UTC-8, Bryan Bishop wrote:

On Tue, Jan 22, 2013 at 6:45 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:
> I've always been a big fan of wiki's, but I haven't really seen them being
> very effective for something like this. No experience with Gitorious. Any
> other suggestions?

How do you feel about how the reprap project core contributors
contribute together?

Well, I can't say I've ever looked into what collaboration tools they use, but I like the results! ;-)

Whatever we decide on, I would like it be something that is accessible to the average wetlab biologist though, not just the average person who already has experience developing collaborative open source projects. I recognize that may be a high bar to set for useability...

Patrik

[Patrik D'haeseleer]

On Tuesday, January 22, 2013 2:22:45 PM UTC-8, Cathal Garvey wrote:

I hadn't actually looked when I asked for it to be hosted everywhere,
it's just that Instructables as a platform is generally very naggy, and
prevents non-payers from doing simple things like zooming in, etc.

By the way - if anyone is in need a free 1yr Pro membership on Instructables, let me know! One of the benefits of "winning a contest, being featured, or just generally being awesome".

Patrik

[Pieter]

At our next meetup we will build the printer using the instructable in the Open Wetlab at Waag Society Amsterdam. http://www.meetup.com/Dutch-DIY-Bio/events/109027502/

We are brainstorming on what else to print, besides patterns. For example, printing a grid of cell cultures and simulate Conway's Game of Life. I am looking for additional ideas that leverage printing living cells on a 2D surface. What else can you think of?

[Patrik D'haeseleer]

Hoi Pieter!

So exciting to hear that you're going to try replicate our bioprinter! Will this just be a one-evening event, or are you planning to work on this over a longer period of time? If you only have the one evening, then just getting all the hardware assembled, wired up and working will probably take up all your time.

Implementing Conway's Game of Life would be really cool, but very challenging. Basically you'd have to start with a significant synthetic biology project, genetically engineering the cells to be printed such that they emit some short-range diffusible signal, and then engineer some logic into the cells so they respond to that signal in a very non-linear fashion that mimics Conway's rules. Doing all that would likely take several months even for a competent academic team. And then you haven't even started printing yet...

An easier project along the same lines would be to engineer two strains of E. coli to implement a negative feedback loop: strain A produces a diffusible signal that turns ON strain B, and strain B produces a diffusible signal that turns OFF strain A. Then watch the interesting spatial patterns develop. See if you can tune the parameters to generate various animal coat patterns: zebra, leopard, giraffe, etc.

There's a bunch of real scientific research questions you could try to address with a bioprinter like this as well: isolate some cross-feeding microorganisms that seem to crossfeed metabolites between each other, and print them at different distances and in different spatial arrangements with respect to each other.

One big limitation of our current bioprinter is that you can only print with one "ink" at a time. That is why we are currently working on replacing the inkjet print head with a couple of syringe pumps. That should open up a whole other range of applications as well.

Patrik

[Avery louie]

If you can get a hold of something with quorum sensing and color out it would be neat to print.

thats what I would do if i had a bioprinter :)

--A

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[Patrik D'haeseleer]

Yes indeed! Even just printing a gradient of Vibrio fisheri cells would make for an interesting experiment.

I do recommend just starting with E. coli (with or without GFP) for your first experiment though. You can also mix in some food dye or even some of the original inkjet ink into the cell culture while printing, so you can instantaneously verify that the print head is working while bioprinting.

I just finished up some simple experiments showing that you can mix in at least up to one part food dye or inkjet ink in 5 parts E. coli liquid culture, without any noticeable qualitative effects on growth of E. coli colonies after streaking or bioprinting on a plate. Still need to write that up...

Patrik