RE: [DIYbio] openPCR

[PCR Assays]

Marketing Pitch:  Probably won’t go through.

 

Fluoresentric is able to provide you with all of the kit ingredients

Contact us at Fluoresentric.com

 

 

From: diy...@googlegroups.com [mailto:diy...@googlegroups.com] On Behalf Of jcrubino
Sent: Monday, December 24, 2012 5:56 PM
To: diy...@googlegroups.com
Subject: [DIYbio] openPCR

 

What is the cost per run on the openPCR or costs associated with operating the openPCR?

Looking to explore the family tree if possible.

 

Thanks 

 

JR

 

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[Nathan McCorkle]

very cheap, less than a dollar when you price out primers + PCR mastermix + DNA spin-columns

On Mon, Dec 24, 2012 at 4:56 PM, jcrubino <james....@gmail.com> wrote:

What is the cost per run on the openPCR or costs associated with operating the openPCR?

Looking to explore the family tree if possible.

Thanks 

JR

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[Josiah Zayner]

The problem isn't really the cost per run it is the initial investment of buying primers, DNA extraction kits, polymerase, DNA clean up kits.

Initial investment is probably around $400 not including sequencing or the PCR machine.
Buy a PCR machine on ebay and save yourself $400+.

[Cathal Garvey]

DNA Cleanup kits not required for straight PCR; rarely necessary in fact.

You ought to be very careful buying PCR machines on Ebay; they are often only tested to turn on, but if there's trouble, the trouble sets in when the machine heats or cools.

Given the shipping costs of getting such a giant heat-sink sent to you for proper testing, you can end up screwing yourself on second-hand equipment.

There's an old argument on the lists about this issue, but I for one suggest supporting community-designed, built and managed hardware that you can rely on community support for. The best available hardware fitting this description is the OpenPCR.

Of course, if cost is an issue, you can always build your own! :)

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[Sung won Lim]

Hmm.

I just want to add that I've bought about five pcr machines off ebay and never had any issues with them so far.

-sung

[Cathal Garvey]

My paranoia may be accentuated, as ever, by the high shipping costs and lead times to Ireland.

[Josiah Zayner]

Sure it is not required for PCR but PCR is mostly useless for creating a family tree or genetic tree without sequencing. And sending PCR products without cleaning them up is a recipe for disaster. They still have polymerase, free nucleotides &c.

I have purchased PCR machines off eBay and never had a problem.

[Josiah Zayner]

For sure if one is paying overseas shipping for such a heavy item it might enter into the realm of not being worth it in the slightest.

[Cathal Garvey]

Ah, I'd forgotten the sequencing part, sorry! :) Was just imagining some fingerprinting and DIY gels.

[Matt Lawes]

For clean up prior to (sanger) sequencing you can treat the PCR reaction with an enzyme mix of Exonuclease I and Shrimp Alkaline Phosphatase. Exo chews up single stranded DNA (including unused primers) , SAP dephosphorylates unused nucleotides.
There is even a branded premix of the enzymes called ExoSAP-It from Affymetrix/USB. (disclosure: I used to work for Affymetrix).

>matt

Sent from my Verizon Wireless 4G LTE DROID

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