(transfected) Insect cell -> entire organism

[Mega]

Hey,

I read that it would be quite easy to add genes to insect cells using the piggyBac transposon. Frequently used for recombinant protein expression in insect cell lines.
So I was wondering how you could get an insect out of those cells. Do you have to transfect insect sperm? Insects are not plants, so you probably can't use any cell for reproducing an entire organism?

[Nathan McCorkle]

yes you want to hit the germline to get expression in the whole
organism (or at least to get the new DNA in the whole organism, you
may add DNA that only expresses in one body area)

theoretically you could also do somatic cell nuclear transfer (SCNT)
from a modified cell to an embryo

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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Andreas Sturm]

>theoretically you could also do somatic cell nuclear transfer (SCNT)
>from a modified cell to an embryo

Of course, but this seems even more complicated ;)

2012/7/30 Nathan McCorkle <nmz...@gmail.com>

[Cathal Garvey]

Not so much complicated as extremely difficult. You need a
micromanipulator and microneedle for one thing, and then you need the
manual skill to use one effectively. Finally, you need lots and lots of
insect ova! :)

This is routine for making transgenic fruit flies, for example. I was
interested in trying it with sea monkies (Artemia salina) for a while
until I discovered how hard it is to get microneedles/micromanipulators.
I'm leaving it for later!

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[Andreas Sturm]

Microinjection?

http://www.youtube.com/watch?v=h-Bfc1GPWpE

Looks cool... But, it will be hard to build it in a diy way... 

2012/7/31 Cathal Garvey <cathal...@gmail.com>