lyophilization help

[biowarrior]

Hi i got a question. I'm currently decellurizing the kidney from a mouse and so far the
Extracellularmatrix is looking more transparent.... I want to lypholize the ECM to preserve it.. I dont
have access to a freeze dryer(too expensive). I read somewhere that you can use 40% Glycerol and beads and it could be stored at -20C but i don't know if that method would work with a sheet of ECM... Please give me your input on this subject....

thanks

perry

[Cathal Garvey]

Untested to from an English friend: place pre-frozen sample in a ziplock bag with a freshly opened disposable iron oxide heat patch. Seal and place in freezer (but not near other samples). Reaction strips h2o from ice by sublimation. Not much heat generated if samples are small, because heat reaction is limited by available water vapour.

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[Forrest Flanagan]

That's not how the heat patches I've used work. They're normally full of fine iron powder, the reaction is limited by available oxygen. Heat is generated as iron rusting (combustion) is exothermic.

I've made decent desiccating devices based on calcium chloride, the common road salt variety. Just seal it in a container and don't let the stuff you're drying actually touch it.

I don't think it'll work well for your case, though. What might work is to get some epsom salt, MgSO4*7H2O, pour it in a nonstick pan, put it in your oven set to 550F or higher for an hour or so, and then bust up the resultant salt cake before it gets a chance to cool or grab any moisture from the atmosphere. Bust it up a bit, get it in a very airtight container, bust it up more by shaking the container, cool the salt in the container, and then seal the prefrozen rat brain in the airtight container such that the brain is isolated but atmosphere is shared.

[Forrest Flanagan]

Then stick that in the freezer.

[Forrest Flanagan]

I'd try it with a slice of frozen banana or something first

[biorocks]

ok guys thanks for all the help... i appreciate it...and i will try both suggestions!

[Shane Karnik]

if you have access to a vacuum pump, cold trap, and liquid nitrogen or acetone and dry ice, you can lyophilize tissue pretty easy.