Is it legal to reproduce an electrospray device?

[Mega]

Guys,

Here (Chris mentioned it some time ago) http://www.nature.com/srep/2012/120228/srep00289/full/srep00289.html#/t1  is a device that can reach transformation efficiencies around 67 % using just water, NaCl, electricity.

If I built a deivce like the one described, would that be stealing of IP ?


If not so, there's a problem:
The cells have to be attached to the petri dish. In the paper this is done with Polyethyleneimine.
When they can float around, the energy is not going to reach the cells in the right way and it won't work.
Polyethyleneimine is very toxic.

Is there a DIY-approach to bind the cells to the dish without toxic or hard-to-get substances??


Regards,

[Nathan McCorkle]

On Mon, Apr 2, 2012 at 3:13 AM, Mega <masters...@gmail.com> wrote:
> If I built a deivce like the one described, would that be stealing of IP ?

Depends if its patented in your country, or if your country follows
patents of other countries

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Andreas Sturm]

Thank you!

The same is true for non-commercial issues i.e. if I don't sell it but use it for myself?

2012/4/2 Nathan McCorkle <nmz...@gmail.com>

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[Forrest Flanagan]

AFAIK, if it's for yourself, you can do whatever you want with anyone's IP.

[Bryan Bishop]

On Mon, Apr 2, 2012 at 10:55 AM, Forrest Flanagan <soleno...@gmail.com> wrote:

AFAIK, if it's for yourself, you can do whatever you want with anyone's IP.

Not quite. Apparently in U.S. law that still counts as patent infringement. See 35 U.S.C. 271.

"Except as otherwise provided in this title, whoever without authority makes, uses, offers to sell, or sells any patented invention, within the United States, or imports into the United States any patented invention during the term of the patent therefor, infringes the patent."

http://www.uspto.gov/web/offices/pac/mpep/documents/appxl_35_U_S_C_271.htm

But in most cases, nobody cares. I don't recall if there has been any cases brought before a judge that tested whether or not non-revenue-generating infringement can be fined.

While looking up a specific quote to that effect on Wikipedia, this caught my eye:

http://en.wikipedia.org/wiki/Patent_infringement#Patent_infringement_insurance

... so maybe we should start dolling out patent infringement insurance to open source hardware builders (who aren't selling, just building or making related kits; and who don't own a patent on their work).

- Bryan
http://heybryan.org/
1 512 203 0507

[Simon Quellen Field]

The government is not going to go after you.

The patent owner has to sue you.

They can sue to stop you, or they can try to prove damages.

But since it would probably not be in their interest to waste lawyer money on you, they

might just send you a letter asking you not to. But most likely, they will never know you

exist.

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[Avery louie]

You can almost definitely make it.  The point of patenting technology is full disclosure of the discovery so that others can do research on it/work with it.  It is to prevent groundbreaking tech from being secreted away, it is kind of like a contract between the government and the inventor saying "you have commercial rights to this for 20 years, as long as you let other people know what is going on"

Simon is pretty much right.

--A

[Cathal Garvey]

In Europe, it's almost always legal to make use of patented stuff for personal applications. That means, unless you're making a profit somehow, either by using or selling the device, you probably don't have to worry about it. This may be a different matter in specific countries; "Your Milage May Vary". :)

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[Andreas Sturm]

OK, thank you!!

Has anyone an idea how to bind cells to the dish in a low-tech maner without using polyethyleneimine?
Without that, the idea is not really feasible...

2012/4/2 Cathal Garvey <cathal...@gmail.com>

[mad_casual]

Has poly-lysine been considered?

2012/4/2 Cathal Garvey <cathal...@gmail.com>

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[Andreas Sturm]

No, didn't know that. thank you.
Wikipedia doesn't say it's toxic. That's good and makes it a candidate for that application.

Yet it should be hard to get for average joe. I'm looking for things like... don't know... honey or some glue.... Something that you can buy in the store

2012/4/3 mad_casual <ademl...@gmail.com>

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[Cathal Garvey]

Anyone out there know enough biochemistry to suggest a way of safely polymerising health-store bought lysine to make polylysine?

Andreas Sturm <masters...@gmail.com> wrote:

>>>>>> Get a free science project every week! "http://scitoys.com/**
>>>>>> newsletter.html <http://scitoys.com/newsletter.html>"

>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Mon, Apr 2, 2012 at 9:10 AM, Bryan Bishop
><kan...@gmail.com>wrote:
>>>>>>
>>>>>>> On Mon, Apr 2, 2012 at 10:55 AM, Forrest Flanagan <
>>>>>>> soleno...@gmail.com> wrote:
>>>>>>>
>>>>>>>> AFAIK, if it's for yourself, you can do whatever you want with
>>>>>>>> anyone's IP.
>>>>>>>
>>>>>>>
>>>>>>> Not quite. Apparently in U.S. law that still counts as patent
>>>>>>> infringement. See 35 U.S.C. 271.
>>>>>>>
>>>>>>> "Except as otherwise provided in this title, whoever without
>>>>>>> authority makes, uses, offers to sell, or sells any patented
>invention,
>>>>>>> within the United States, or imports into the United States any
>patented
>>>>>>> invention during the term of the patent therefor, infringes the
>patent."
>>>>>>>

>>>>>>> http://www.uspto.gov/web/**offices/pac/mpep/documents/**
>>>>>>>
>appxl_35_U_S_C_271.htm<http://www.uspto.gov/web/offices/pac/mpep/documents/appxl_35_U_S_C_271.htm>

>>>>>>>
>>>>>>> But in most cases, nobody cares. I don't recall if there has
>been any
>>>>>>> cases brought before a judge that tested whether or not
>>>>>>> non-revenue-generating infringement can be fined.
>>>>>>>
>>>>>>> While looking up a specific quote to that effect on Wikipedia,
>this
>>>>>>> caught my eye:

>>>>>>> http://en.wikipedia.org/wiki/**Patent_infringement#Patent_**
>>>>>>>
>infringement_insurance<http://en.wikipedia.org/wiki/Patent_infringement#Patent_infringement_insurance>

>>>>>>> ... so maybe we should start dolling out patent infringement
>>>>>>> insurance to open source hardware builders (who aren't selling,
>just
>>>>>>> building or making related kits; and who don't own a patent on
>their work).
>>>>>>>
>>>>>>> - Bryan
>>>>>>> http://heybryan.org/
>>>>>>> 1 512 203 0507
>>>>>>>
>>>>>>> --
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>>>>>>> group/diybio?hl=en
><http://groups.google.com/group/diybio?hl=en>.

>>>>>>>
>>>>>>
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>>>>>
>>>>
>>>>
>>>>
>>>> --
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[Jeswin]

On Wed, Apr 4, 2012 at 4:28 AM, Cathal Garvey <cathal...@gmail.com> wrote:
> Anyone out there know enough biochemistry to suggest a way of safely polymerising health-store bought lysine to make polylysine?
>

From Japan: Food-grade PolyLysine Preservative $13/5g (Postage included?)

http://www.wholesalegroup.com.au/nata.html

Not sure about the quality, etc.

[mad_casual]

I don't want to say that epsilon-poly-lysine won't work, but I know the attachment protocols I've read and used worked with alpha-poly-lysine. I know poly-ornithine and poly-arginine can be used to largely the same effect as poly-lysine. I would assume that random polypeptides would work to a modest degree. I'm sure some textbook or paper somewhere has a table of surface modifiers that have been tried and their respective effectiveness, but I don't (know that I) own it.

(I think that) Lysine will be relatively hard to polymerize cheaply/safely/easily de novo as it has two primary amines of similar reactivity. That is, when a-poly-lysine is produced in vitro, lysine precursors that have protected epsilon amines are used. I will say that the coatings I've done, 100 mg is A LOT of poly-lysine and a good portion of that (90%) is used to bias the equillibrium and drive the reaction rather than actually reacting.