Hanabi or Genial Cytogenetic Automated systems

[Chinly]

Hi all,
very appreciated if any of you can share yours experiences or comments on Hanabi or Genial cytogenetic automated system. Hanabi also intruduce their metaphase spreader to us which claim can process 1-5 slides in on time. I would like to know from any user of their pro and con for these equipments.
Thank you

regards
Chinly.

[Peh GuAt Lian]

Which of genial system are u interested in? Tube harvest or insitu?

Sent from my iPhone

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[Gollin, Susanne M]

Does anyone have a good method for harvesting/karyotyping cells growing on gelatin? We never can get good metaphases from murine lung or aortic endothelial cell cultures and need some suggestions.  Does the colcemid get bound up by the gelatin? How can we dissolve the gelatin while not harming the cells? Please advise.  We see nice cell colonies, but perhaps they divide slowly.

Susanne

Susanne M. Gollin, Ph.D., FFACMG
Professor of Human Genetics, Otolaryngology,  
     and Pathology
University of Pittsburgh Graduate School of 
     Public Health
(412) 624-5390
gol...@pitt.edu

[Phan Chin Lee]

Tube harvest
regards
chinly

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From: guatl...@yahoo.com.sg
Subject: Re: Hanabi or Genial Cytogenetic Automated systems
Date: Fri, 9 Nov 2012 10:25:14 +0800
To: cytogenetics-methods...@googlegroups.com

[rekha s]

I am interested in bone marrow and PB ckaryotyping

[Peh GuAt Lian]

I'm sorry. I've never tried the tube harvester. Only the in-situ dish harvestor.

Sent from my iPhone

[Michael Babineau]

My lab has 2 Hanabi metaphase harvesters. The harvesters are built for 24 samples but my protocol is only to do 16 max, for QC. Run time is about 2hrs10min, on a busy Saturday by myself I've harvested 80 bone marrow samples within 9 hours. No major complaints, needs maintenance on occasion if pieces finally wear out. We've had some trouble with contamination at one point from using tap water, be sure to use DH2O for cleaning at the end of the day. Any other questions let me know!

On Friday, November 9, 2012 2:13:29 AM UTC-5, Phan Chin Lee wrote:

Tube harvest
regards
chinly

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From: guatl...@yahoo.com.sg
Subject: Re: Hanabi or Genial Cytogenetic Automated systems
Date: Fri, 9 Nov 2012 10:25:14 +0800

To: cytogenetics-methods-and-trouble-shooting@googlegroups.com

Which of genial system are u interested in? Tube harvest or insitu?

Sent from my iPhone

On Oct 22, 2012, at 10:54 AM, Chinly <karun...@googlemail.com> wrote:

Hi all,
very appreciated if any of you can share yours experiences or comments on Hanabi or Genial cytogenetic automated system. Hanabi also intruduce their metaphase spreader to us which claim can process 1-5 slides in on time. I would like to know from any user of their pro and con for these equipments.
Thank you

regards
Chinly.

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[Phan Chin Lee]

Hi Michael,
Noted. Thank you for sharing.
 
chinly
 

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Date: Sat, 30 Mar 2013 06:41:30 -0700
From: michaelc...@gmail.com
To: cytogenetics-methods...@googlegroups.com
CC: cua...@hotmail.com

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[Cristiano]

Hi Chinly,

we have one Genial Harvester CS24 for 24 probes since one year in our lab (Germany). The harvester is running daily with different amounts of probes, sometimes 2 runs a day are performed. We had no problems with the system yet, and the quality of the suspensions are comparable to manual handling, sometimes even better. As a nice side aspect our FISH performed on automated harvested probes look better than on manually harvested probes. We mainly have bone marrow probes and many peripheral blood probes from CLL-patients. Harvesting of 24 probes takes (depending on protocols) about 2 and 1/2 hours.
Support by Genial is very friendly and quick responding.
If you have more questions dont hesitate to ask.
Regards,

Cristiano