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Error in Creating Karyotype
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Ben Green  
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 More options Sep 17 2012, 2:10 pm
From: Ben Green <bbgreen1...@gmail.com>
Date: Mon, 17 Sep 2012 11:10:14 -0700 (PDT)
Local: Mon, Sep 17 2012 2:10 pm
Subject: Error in Creating Karyotype

Hi all,

This is likely a basic issue, but when trying to create a karyotype of the
bovine genome I am running into some issues within Perl. I have created a
karyotype.cow.conf file and am using the format from the Circos site but am
getting the following errors:

bbgreen$ perl /Applications/circos-0.55/other/circos.conf

Bareword found where operator expected at
/Applications/circos-0.55/other/circos.conf line 3, near "</colors"
  (Might be a runaway multi-line // string starting on line 2)
(Missing operator before lors?)
syntax error at /Applications/circos-0.55/other/circos.conf line 3, near
"</colors"
Can't find string terminator "include" anywhere before EOF at
/Applications/circos-0.55/other/circos.conf line 9.

The following is my circus.conf code if that helps figure out where I am
going wrong (I have moved some .conf files from /etc to a folder I created
(other) for organization):

<colors>
<<include other/colors.conf>>
</colors>

<fonts>
<<include other/fonts.conf>>
</fonts>

<<include ideogram.conf>>
<<include ticks.conf>>

karyotype = other/karyotype.cow.txt

<image>
dir = /tmp
file  = circos-tutorial.png
# radius of inscribed circle in image
radius         = 1500p
background     = white
# by default angle=0 is at 3 o'clock position
angle_offset   = -90
#angle_orientation = counterclockwise
</image>

chromosomes_units           = 1000000

#chromosomes_display_default = yes

chromosomes = hs1;hs2;hs3

anglestep       = 0.5
minslicestep    = 10
beziersamples   = 40
debug           = no
warnings        = no
imagemap        = no

units_ok = bupr
units_nounit = n

Any help is greatly appreciated.

-Ben


 
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Martin Krzywinski  
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 More options Sep 17 2012, 2:48 pm
From: Martin Krzywinski <martin.krzywin...@gmail.com>
Date: Mon, 17 Sep 2012 11:48:53 -0700
Local: Mon, Sep 17 2012 2:48 pm
Subject: Re: [circos] Error in Creating Karyotype

Hi Ben,

You need to use the -conf flag to specify the configuration file.

> bbgreen$ perl -conf /Applications/circos-0.55/other/circos.conf

Your configuration file looks like it's from an older version of
Circos. There have been quite a few changes in v0.6x and I suggest
that you use the latest version of Circos to avoid compatibility
problems in the future.

In the new version you'll find the bovine karyotype file in

data/karyotype/assembly/karyotype.cow.txt

which is based on the Oct 2011 (4.6.1) version. I've attached it here
(colors for chromosomes chr25+ are not defined by default in Circos,
which only defines the human chromosome palette used by UCSC - see
README).

Martin Krzywinski
science + art
http://mkweb.bcgsc.ca

  karyotype.cow.txt
1K Download

  README
2K Download

 
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Ben Green  
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 More options Sep 17 2012, 6:26 pm
From: Ben Green <bbgreen1...@gmail.com>
Date: Mon, 17 Sep 2012 15:26:51 -0700 (PDT)
Local: Mon, Sep 17 2012 6:26 pm
Subject: Re: [circos] Error in Creating Karyotype

> Hi Martin,

Perfect. That fixed the issue. I appreciate the help.

Ben


 
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