> This is marvelous. Thank you for sharing that observation!- Hide quoted text -
>
> - Show quoted text -
Wellkommen, hier ist klene mehr.
Maybe we'll see mice that crave french fries instead of cheese
someday.
(30) Stem Cells Restore Cognitive Abilities Impaired By Brain Cancer
Treatment
Article Date: 14 Jul 2011 - 5:00 PDT
Human neural stem cells are capable of helping people regain learning
and memory abilities lost due to radiation treatment for brain tumors,
a UC Irvine study suggests.
Research with rats found that stem cells transplanted two days after
cranial irradiation restored cognitive function, as measured in one-
and four-month assessments. In contrast, irradiated rats not treated
with stem cells showed no cognitive improvement.
"Our findings provide solid evidence that such cells can be used to
reverse radiation-induced damage of healthy tissue in the brain," said
Charles Limoli, a UCI radiation oncology professor.
Study results appear in the July 15 issue of Cancer Research, a
journal of the American Association for Cancer Research.
Radiotherapy for brain tumors is limited by how well the surrounding
tissue tolerates it. Patients receiving radiation at effective levels
suffer varying degrees of learning and memory loss that can adversely
affect their quality of life.
"In almost every instance, people experience severe cognitive
impairment that's progressive and debilitating," Limoli said.
"Pediatric cancer patients can experience a drop of up to three IQ
points per year."
For the UCI study, multipotent human neural stem cells were
transplanted into the brains of rats that had undergone radiation
treatment. They migrated throughout the hippocampus - a region known
for the growth of new neurons - and developed into brain cells.
Researchers assessed the rats one month and four months after
transplantation, noting enhanced learning and memory abilities at both
intervals.
Additionally, they found that transplanting as few as 100,000 human
neural stem cells was sufficient to improve cognition after cranial
irradiation. Of cells surviving the process, about 15 percent turned
into new neurons, while another 45 percent became astrocytes and
oligodendrocytes - cells that support cerebral neurons.
Most notably, Limoli said, he and his colleagues discovered that about
11 percent of the engrafted cells expressed a behaviorally induced
marker of learning, indicating the functional integration of those
cells into memory circuits in the hippocampus.
"This research suggests that stem cell therapies may one day be
implemented in the clinic to provide relief to patients suffering from
cognitive impairments incurred as a result of their cancer
treatments," Limoli said. "While much work remains, a clinical trial
analyzing the safety of such approaches may be possible within a few
years, most likely with patients afflicted with glioblastoma
multiforme, a particularly aggressive and deadly form of brain
cancer."
Munjal Acharya, Lori-Ann Christie, Mary Lan and Erich Giedzinski of
UCI and John Fike and Susanna Rosi of UC San Francisco contributed to
the study, which was funded by the California Institute for
Regenerative Medicine, the National Institutes of Health and the U.S.
Department of Energy.
(32) Stem Cells Restore Cognitive Abilities Impaired By Brain Cancer
Treatment
Article Date: 14 Jul 2011 - 5:00 PDT
Human neural stem cells are capable of helping people regain learning
and memory abilities lost due to radiation treatment for brain tumors,
a UC Irvine study suggests.
Research with rats found that stem cells transplanted two days after
cranial irradiation restored cognitive function, as measured in one-
and four-month assessments. In contrast, irradiated rats not treated
with stem cells showed no cognitive improvement.
"Our findings provide solid evidence that such cells can be used to
reverse radiation-induced damage of healthy tissue in the brain," said
Charles Limoli, a UCI radiation oncology professor.
Study results appear in the July 15 issue of Cancer Research, a
journal of the American Association for Cancer Research.
Radiotherapy for brain tumors is limited by how well the surrounding
tissue tolerates it. Patients receiving radiation at effective levels
suffer varying degrees of learning and memory loss that can adversely
affect their quality of life.
"In almost every instance, people experience severe cognitive
impairment that's progressive and debilitating," Limoli said.
"Pediatric cancer patients can experience a drop of up to three IQ
points per year."
For the UCI study, multipotent human neural stem cells were
transplanted into the brains of rats that had undergone radiation
treatment. They migrated throughout the hippocampus - a region known
for the growth of new neurons - and developed into brain cells.
Researchers assessed the rats one month and four months after
transplantation, noting enhanced learning and memory abilities at both
intervals.
Additionally, they found that transplanting as few as 100,000 human
neural stem cells was sufficient to improve cognition after cranial
irradiation. Of cells surviving the process, about 15 percent turned
into new neurons, while another 45 percent became astrocytes and
oligodendrocytes - cells that support cerebral neurons.
Most notably, Limoli said, he and his colleagues discovered that about
11 percent of the engrafted cells expressed a behaviorally induced
marker of learning, indicating the functional integration of those
cells into memory circuits in the hippocampus.
"This research suggests that stem cell therapies may one day be
implemented in the clinic to provide relief to patients suffering from
cognitive impairments incurred as a result of their cancer
treatments," Limoli said. "While much work remains, a clinical trial
analyzing the safety of such approaches may be possible within a few
years, most likely with patients afflicted with glioblastoma
multiforme, a particularly aggressive and deadly form of brain
cancer."
Munjal Acharya, Lori-Ann Christie, Mary Lan and Erich Giedzinski of
UCI and John Fike and Susanna Rosi of UC San Francisco contributed to
the study, which was funded by the California Institute for
Regenerative Medicine, the National Institutes of Health and the U.S.
Department of Energy.
(34) Implanted Neurons, Grown In The Lab, Take Charge Of Brain
Circuitry
Main Category: Neurology / Neuroscience
Article Date: 22 Nov 2011 - 1:00 PST
Among the many hurdles to be cleared before human embryonic stem cells
can achieve their therapeutic potential is determining whether or not
transplanted cells can functionally integrate into target organs or
tissues.
Writing today (Monday, Nov. 21) in the Proceedings of the National
Academy of Sciences, a team of Wisconsin scientists reports that
neurons, forged in the lab from blank slate human embryonic stem cells
and implanted into the brains of mice, can successfully fuse with the
brain's wiring and both send and receive signals.
Neurons are specialized, impulse conducting cells that are the most
elementary functional unit of the central nervous system. The 100
billion or so neurons in the human brain are constantly sending and
receiving the signals that govern everything from walking and talking
to thinking. The work represents a crucial step toward deploying
customized cells to repair damaged or diseased brains, the most
complex human organ.
"The big question was can these cells integrate in a functional way,"
says Jason P. Weick, the lead author of the new study and a staff
scientist at the University of Wisconsin-Madison's Waisman Center. "We
show for the first time that these transplanted cells can both listen
and talk to surrounding neurons of the adult brain."
The Wisconsin team tested the ability of their lab grown neurons to
integrate into the brain's circuitry by transplanting the cells into
the adult mouse hippocampus, a well-studied region of the brain that
plays a key role in processing memory and spatial navigation. The
capacity of the cells to integrate was observed in live tissue taken
from the animals that received the cell transplants.
Weick and colleagues also reported that the human neurons adopted the
rhythmic firing behavior of many brain cells talking to one another in
unison. And, perhaps more importantly, that the human cells could
modify the way the neural network behaved.
A critical tool that allowed the UW group to answer this question was
a new technology known as optogenetics, where light, instead of
electric current, is used to stimulate the activity of the neurons.
"Previously, we've been limited in how efficiently we could stimulate
transplanted cells. Now we have a tool that allows us to specifically
stimulate only the transplanted human cells, and lots of them at once
in a non-invasive way," says Weick.
Weick explains that the capacity to modulate the implanted cells was a
necessary step in determining the function of implanted cells because
previous technologies were too imprecise and unreliable to accurately
determine what transplanted neurons were doing.
Embryonic stem cells, and the closely related induced pluripotent stem
cells can give rise to all of the 220 types of tissues in the human
body, and have been directed in the lab to become many types of cells,
including brain cells.
The appeal of human embryonic stem cells and induced pluripotent cells
is the potential to manufacture limitless supplies of healthy,
specialized cells to replace diseased or damaged cells. Brain
disorders such as Parkinson's disease and amyotrophic lateral
sclerosis, more widely known as Lou Gehrig's disease, are conditions
that scientists think may be alleviated by using healthy lab grown
cells to replace faulty ones. Multiple studies over the past decade
have shown that both embryonic stem cells and induced cells can
alleviate deficits of these disorders in animal models.
The new study opens the door to the potential for clinicians to deploy
light-based stimulation technology to manipulate transplanted tissue
and cells. "The marriage between stem cells and optogenetics has the
potential to assist in the treatment of a number of debilitating
neurodegenerative disorders," notes Su-Chun Zhang, a UW-Madison
professor of neuroscience and an author of the new PNAS report. "You
can imagine that if the transplanted cells don't behave as they
should, you could use this system to modulate them using light."
Sheng Li Xue Bao. 2010 Feb 25;62(1):79-85.
[Anti-aging effect of transplantation of mouse fetus-derived
mesenchymal stem cells.]
[Article in Chinese]
Li J, Zhang Y, Liu GX.
Institute of Hematonosis, Medical School, Jinan University, Guangzhou
510632, China. E-mail:
tli...@jnu.edu.cn.
To determine the role of allogeneil graft of mesenchymal stem cells in
mammalian longevity, mesenchymal stem cells were isolated from BALB/c
mouse uterine-incision delivery fetus by two successive density
gradient centrifugations, and then were purified and amplified by
adherent culture. Identified P1 mesenchymal stem cells were injected
(i.v.) through vena caudalis into the 15-month-old female BALB/c mice
three times. The mice were evaluated with ultrasoundcardiogram,
autopsy, score of cardiac, skin, lung, kidney, colon histopathology
and serum total superoxide dismutase activity, maleic dialdehyde
content, glutathione peroxidase activity. The results showed that
after transplantation, the long-term surviving stem cells were found
to be located in many organ tissues with in situ Y chromosomal
hybridization dyeing. Median life span was increased in these animals
after transplantation. Skin, cardiac, lung, kidney and colon pathology
development were delayed. The retrogradation of heart function was
attenuated, the increase of heart mass index (the mass of heart/the
mass of the body), and serum maleic dialdehyde content, the decrease
of spleen mass index (the mass of spleen/the mass of the body), serum
total superoxide dismutase activity and glutathione peroxidase
activity were reduced three months after transplantation (all P<0.05).
These results support the idea that longevity can be enhanced by
transplantation of mesenchymal stem cells and reinforce the hypothesis
of mesenchymal stem cell as antiager.
PMID: 20179893