FastQCFastQC Report
Mon 21 Aug 2017
RP.36_S91_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRP.36_S91_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313658
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA135264.312340192183844No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA68822.1941095078078674No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA58781.8740156476161933No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA51511.6422345357044934No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA48031.5312856678292919No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA40631.2953599143015642No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGCA39461.2580581397573154No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA38401.2242633696573975No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA34091.0868525591567886No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA31911.0173501074418634No Hit
TACGGAGGGTGCAAACGTTGTTCGGAATTATTGGGCGTAAAGAGCATGTA30240.9641074036052006No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA26940.858897270275268No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA23340.744122579369887No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA21290.6787647692709895No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA20530.6545345567465201No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA19470.6207397866466023No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA19350.6169139636164229No Hit
TACGGAGGGTCCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA19170.611175229071154No Hit
TACCAGCTCTCCGAGTGGTAGGGATGATTATTGGGCTTAAAGCGTCCGTA18590.5926837510919537No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGTCTA17850.5690911757391809No Hit
GACAGAGGTGGCAAGCGTTGTTCGGATTTACTGGGCTTAAAGGGCGTGTA16470.5250942108921182No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA16460.5247753923062699No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTACGTA16440.5241377551345733No Hit
TACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA16210.5168049276600629No Hit
TACGGAGGGTGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA16040.5113850117006421No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA14590.46515631675264135No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA14290.45559175917719297No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA13870.4422013785715652No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA12970.41350770584521995No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA12810.40840660847164745No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA12690.4045807854414681No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA12150.3873645818056609No Hit
TACGGAGGGTCCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGCA11960.3813070286745436No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCATGTA11920.3800317543311505No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11790.3758871127151228No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA11780.37556829412927456No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA11210.35739563473592256No Hit
AACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCACGTA11200.35707681615007425No Hit
AACCAGCACCTCAAGTGGTCAGGATGATTATTGGGCCTAAAGCATCCGTA11180.35643917897837774No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA10850.34591816564538447No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA10730.3420923426152051No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTACGTA10630.3389041567567223No Hit
TACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA10530.33571597089823946No Hit
TACGGAGGGGGCGAGCGTTGCTCGGAATTACTGGGCGTAAAGGGTCCGCA10520.3353971523123912No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA9930.31658685574734263No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA9850.3140363070605564No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATCACTGGGCGTAAAGAGCGCGTA9530.30383411231341145No Hit
CACGTAGGATTCGAACGTTATCCGGAATTACTGGGCGTAAAGCGCGTGCA9230.294269554737963No Hit
TACGGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA9080.28948727595023876No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA8730.278328625445549No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA8400.2678076121125557No Hit
AACGTAGGACGCGAACGTTATCCGGAATTACTGGGCGTAAAGGGCGCGCA8240.2627065147389832No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGCA8200.2614312403955901No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA8060.2569677801937142No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA7970.25409841292107965No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATTATTGGGTTTAAAGGGTCCGTA7920.25250431999183826No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCATGTA7910.25218550140598994No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGCA7350.23433166059848623No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGTGTA7190.22923056322491378No Hit
CACCAGCCCCTCGAGTGGTGGGGATGATTATTTGGCCTAAAGCGTCCGTA6830.21775309413437563No Hit
CACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGACGCGCA6760.21552136403343772No Hit
AACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCACGTA6530.20818853655892722No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGCA6490.2069132622155341No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTCACTGGGTATAAAGGGTGCGCA6420.20468153211459614No Hit
TACGGAGGATCCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA6310.20117452767026506No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGTGTA6160.19639224888254084No Hit
AACGTAGGACGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCAGGCA6150.19607343029669258No Hit
GACGAACCGTCCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA6140.1957546117108443No Hit
TACAGAGGTGGCGAACGTTGTTCGGATTTACTGGGCGTAAAGGGTCCGCA6130.19543579312499604No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGCA6130.19543579312499604No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGAGCGCGTA6100.19447933736745115No Hit
TACGGAGGGGGCAAGCGTTATTCGGATTTACTGGGCGTAAAGCGCACGTA6100.19447933736745115No Hit
TACGGGGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGTGTA6070.19352288160990633No Hit
TACGGATGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA5820.1855524169636993No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGAGCACGTA5770.18395832403445792No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCTCGTA5730.18268304969106477No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA5700.18172659393351995No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5690.18140777534767166No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA5630.17949486383258198No Hit
TACGAACCGTACGAACGTTATTCGGAATCACTGGGCTTAAAGAGTGCGTA5620.17917604524673372No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5560.177263133731644No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA5490.17503140363070607No Hit
GACGAAGGTGGCAAGCGTTGTTCGGTATCACTGGGCTTAAAGCGTGTGTA5430.17311849211561636No Hit
TACGGAGGGTGCGAACGTTGTTCGGAATTATTGGGCGTAAAGAGCATGTA5330.16993030625713357No Hit
TACGGGGGGTGCGAGCGTTATTCGGAATTACTGGGCGTAAAGAGCGCGTA5240.16706093898449906No Hit
TACGAAGGTCCCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTATGTA5140.16387275312601624No Hit
TACGGATGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA5070.16164102302507827No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGTGTA4940.15749638140905062No Hit
AACGTAGGACGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCGCGCA4910.15653992565150576No Hit
TACGGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA4890.15590228847980922No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGGGCGCGTA4860.1549458327222644No Hit
AACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA4810.15335173979302297No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA4790.1527141026213264No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA4750.15143882827793329No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA4700.1498447353486919No Hit
AACGTAGGACGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA4610.14697536807605738No Hit
GACGGAGGGCGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA4550.14506245656096767No Hit
TACGGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA4550.14506245656096767No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA4460.14219308928833316No Hit
TACAGAGGGTGCAAACGTTGCTCGGATTTACTGGGCGTAAAGCGCGTGTA4450.14187427070248487No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA4440.1415554521166366No Hit
TACATATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA4390.1399613591873952No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA4370.13932372201569862No Hit
GACGAAGGTGGCAAGCGTTGTTCGGTATTACTGGGCTTAAAGCGTGTGTA4370.13932372201569862No Hit
CACGAACTGCGCGAACGTTATTCGGAATCACTGGGCTTAAAGAGTTCGTA4330.1380484476723055No Hit
TACCAGCTCTCCGAGTAGTGTGGATGTTTATTGGGCCTAAAGCATCCGTA4270.13613553615721583No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA4200.13390380605627786No Hit
TACGGGGGGAGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGTGTA4140.13199089454118818No Hit
TACGTAGGAGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTGCA3980.12688979716761567No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA3970.1265709785817674No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTACAGGGTGCGTA3790.12083224403649837No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA3740.11923815110725694No Hit
TACGGGGGGTGCTAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGTGTA3690.11764405817801554No Hit
TACGAAAGGTGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGAGATCGTA3670.11700642100631899No Hit
AACGTAGGACACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA3640.11604996524877415No Hit
CACGTAGGATCCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3640.11604996524877415No Hit
TACAGAGGTGGCGAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA3620.11541232807707758No Hit
TACGGGGGGAGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGCGCGTA3620.11541232807707758No Hit
CACGGGGGGCGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA3610.11509350949122929No Hit
TACGGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3610.11509350949122929No Hit
TACTAGCGGTCCAAGTCGCAGCCATTTTTATTGGGTCTAAAACATCCGTA3610.11509350949122929No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGAGCGTGTA3600.11477469090538102No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA3590.11445587231953273No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA3560.11349941656198789No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3550.11318059797613962No Hit
GACGGAGGGCGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGGGCA3500.11158650504689822No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA3460.11031123070350508No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3310.10552895191578088No Hit
TACGGAGGGTGCGAACGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3310.10552895191578088No Hit
AACGTAGGACGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA3260.10393485898653948No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGAGCGCGTA3220.10265958464314635No Hit
GACGGAGGGTGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA3190.10170312888560151No Hit
TACGGATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA3140.10010903595636012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG550.0243.988057
CACCTCA1250.0243.988057
ACCCCTA251.6516424E-9243.988058
TACGTAT19550.0243.988051
GTGCCCC251.6516424E-9243.988056
CCCCTCG650.0243.988047
TACCCGC201.5924888E-7243.988041
GGCACCA100.0014940916243.988047
GATCCAG100.0014940916243.988048
GCACCAA100.0014940916243.988048
CTCTGCG100.0014940916243.988047
CACGTAT800.0243.988041
AGGATCC5300.0243.988046
TAGGACA750.0243.988045
TATGTTG100.0014940916243.988045
CACCCGC151.5436033E-5243.988041
TACGTGT100.0014940916243.988041
TACGTAC900.0243.988041
TACGGAG146400.0241.821491
AACGTAG20250.0241.578281
Produced by FastQC (version 0.11.5)