Logging started at 17:53:50 on 25 May 2016
QIIME version: 1.9.1

qiime_config values:
pick_otus_reference_seqs_fp	/home/obrett/my_env/lib/python2.7/site-packages/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta
sc_queue	all.q
pynast_template_alignment_fp	/home/obrett/my_env/lib/python2.7/site-packages/qiime_default_reference/gg_13_8_otus/rep_set_aligned/85_otus.pynast.fasta
cluster_jobs_fp	start_parallel_jobs.py
assign_taxonomy_reference_seqs_fp	/home/obrett/my_env/lib/python2.7/site-packages/qiime_default_reference/gg_13_8_otus/rep_set/97_otus.fasta
torque_queue	friendlyq
jobs_to_start	1
denoiser_min_per_core	50
assign_taxonomy_id_to_taxonomy_fp	/home/obrett/my_env/lib/python2.7/site-packages/qiime_default_reference/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt
temp_dir	/tmp/
blastall_fp	blastall
seconds_to_sleep	1

parameter file values:
assign_taxonomy:reference_seqs_fp	/home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_refs_qiime_ver7_97_s_31.01.2016.fasta
assign_taxonomy:id_to_taxonomy_fp	/home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_taxonomy_qiime_ver7_97_s_31.01.2016.txt
assign_taxonomy:assignment_method	blast
pick_open_reference_otus:prefilter_refseqs_fp	/home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_refs_qiime_ver7_97_s_31.01.2016.fasta
pick_open_reference_otus:suppress_align_and_tree	True
pick_open_reference_otus:prefilter_percent_id	0.6
pick_otus:enable_rev_strand_match	True
parallel:jobs_to_start	1

Input file md5 sums:
filtered_R1.fasta: 5235176b0afb03fb24d78b5d84635e7f
/home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_refs_qiime_ver7_97_s_31.01.2016.fasta: 3bc1bdf6cb1f7146fc97b1af9757ef96

Forcing --suppress_new_clusters as this is reference-based OTU picking.

Executing commands.

# Pick Reference OTUs command 
pick_otus.py -i filtered_R1.fasta -o open_reference_otus4//step1_otus -r /home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_refs_qiime_ver7_97_s_31.01.2016.fasta -m usearch61_ref --enable_rev_strand_match --suppress_new_clusters



*** ERROR RAISED DURING STEP: Pick Reference OTUs
Command run was:
 pick_otus.py -i filtered_R1.fasta -o open_reference_otus4//step1_otus -r /home/obrett/UNITE/sh_qiime_release_s_31.01.2016/sh_refs_qiime_ver7_97_s_31.01.2016.fasta -m usearch61_ref --enable_rev_strand_match --suppress_new_clusters
Command returned exit status: 1
Stdout:

Stderr
Traceback (most recent call last):
  File "/home/obrett/my_env/bin/pick_otus.py", line 1004, in <module>
    main()
  File "/home/obrett/my_env/bin/pick_otus.py", line 897, in main
    otu_prefix=otu_prefix, HALT_EXEC=False)
  File "/home/obrett/my_env/lib/python2.7/site-packages/qiime/pick_otus.py", line 1800, in __call__
    HALT_EXEC=HALT_EXEC
  File "/home/obrett/my_env/lib/python2.7/site-packages/bfillings/usearch.py", line 1844, in usearch61_ref_cluster
    raise ApplicationError('Error running usearch61. Possible causes are '
burrito.util.ApplicationError: Error running usearch61. Possible causes are unsupported version (current supported version is usearch v6.1.544) is installed or improperly formatted input file was provided


Logging stopped at 17:54:02 on 25 May 2016