SUBJECT:
EMF's - 50 hz. GENOTOXIC.....
WILEY INTERSCIENCE -
Volume
20, Issue 6 , Pages 585 - 591
Published Online: 21 Nov 2005
Copyright © 2005 Wiley Periodicals, Inc.
Genotoxic effects of
extremely low frequency (ELF) magnetic fields (MF) evaluated by
the Tradescantia-micronucleus
assay
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Cristina Fatigoni, Luca Dominici, Massimo Moretti
*,
Milena Villarini, Silvano Monarca
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Department of Medical-Surgical Specialities and Public
Health, University of Perugia, Via del Giochetto, I-06126
Perugia (Italy)
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email: Massimo Moretti (massimo.moretti@unipg.it)
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*Correspondence to Massimo
Moretti, Department of Medical-Surgical Specialities and Public
Health, University of Perugia, Via del Giochetto, I-06126 Perugia
(Italy)
Funded by: Italian
Ministry of Labour and Social Policies; Grant Number: 1239
extremely low frequency magnetic fields • ELF-MF •
Tradescantia-micronucleus assay • Trad-MN test •
genotoxicity
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Extremely low frequency (ELF) electric fields (EF) and
magnetic fields (MF) are generated during the production,
transmission, and use of electrical energy. Although
epidemiology studies suggest that there is a cancer risk
associated with exposure to ELF-MF, short-term genotoxicity
assays with bacteria and mammalian cells have produced
inconsistent results. In the present study, we investigated the
possible genotoxicity of ELF-MF by using the
Tradescantia-micronucleus (Trad-MN) assay, a sensitive,
reproducible, well-standardized assay for genotoxicity testing.
A 50 Hz ELF-MF was generated by a laboratory exposure system
consisting of a pair of parallel coils in a Helmholtz
configuration. Exposure of Tradescantia (clone # 4430)
inflorescences to the ELF-MF, at a flux density (B)
corresponding to 1 mT, for 1, 6, and 24 h resulted in a
time-dependent increase in MN frequency. The results indicate
that a 50 Hz MF of 1 mT
field strength is genotoxic
in the Trad-MN bioassay and suggest that this assay
may be suitable as a
biomonitor for detecting the genotoxicity of ELF-MF in the
field. © 2005 Wiley Periodicals, Inc.
Environ Toxicol 20: 585-591, 2005.
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Received: 15 March 2005; Revised: 7 July 2005; Accepted: 19
July 2005
Digital Object Identifier (DOI)
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10.1002/tox.20148 About
DOI
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