Mother Liquor:
1L Propylene Glycol
150ml Deionised Water
115g Agar

For optional addition post-precipitation:
500ml Acetone, Methanol, Ethanol or Isopropanol

Procedure:
Add agar gradually (preferably sifted)
	to propylene glycol and dH2O
Heat solution to 100C and stir until dissolved (1hr+)
When fully dissolved, cool solution to room temperature
	(Opt: Cool to 4C)
	(*Fine* agarose precipitate forms)
(Opt) dilute the slurry with 500-1000ml Acetone/Alcohol
	(Opt: Cool to -20 Overnight!)
Filter or centrifuge the slurry to recover precipitate
(Opt) Wash precipitate by suspending in IPA or 1:1 IPA/PG
	and refiltering/centrifuging.

If used while still damp with IPA/Acetone, will dissolve
and gel at RT. If dried, requires boiling as usual.

Might need to repeat: Examples given indicate that Agar
when purified gives agarose with gel strength of 937
(originally 200), but low-grade SeaKem LE Agarose gives
gel strength of 1400 (original 1054). Ash/Sulfate/EEO for
agarose from raw agar is generally worse than the
low-grade SeaKem Agarose by a modest margin, but repeating
the purification would probably give far better results.

However, recovery from the slurry for the SeaKem agarose
was 61%, so you're really trading high quality for
substantial losses. Recovery of Agarose from raw agar was
21%

Pre-treatment of agar with pectinase might solublise much
agaropectin in water, raising the input purity and calling
for fewer chemical inputs.