FastQCFastQC Report
Sun 8 Jan 2017
MH-S1_S4_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMH-S1_S4_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209925164
Sequences flagged as poor quality0
Sequence length20-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA160000.025.8802011
TCGGAAG229150.018.113252
CGGAAGA233450.017.8037643
CGTATGC135600.016.25800342-43
TCGTATG173900.016.24228942-43
TATGCCG143250.015.26053544-45
TCTCGTA180400.014.3145540-41
GCCGTCT215900.012.91496748-49
ATGCCGT225950.012.61135246-47
CTCGTAT179250.012.29065140-41
GATCGGA91650.011.3424931
GATCTCG231600.011.3056838-39
CGTCTGA258500.08.84226314-15
ACACGTC346350.08.59926212-13
CGGTACG47600.08.510005120
ATCTCGT253650.08.40657938-39
TGCCGTC278900.08.24022746-47
CCGTCTT277500.08.19546248-49
ACGTCTG400450.07.538451714-15
GTATGCC513850.06.15011444-45
Produced by FastQC (version 0.11.2)