(-: Looking for fastq files...fastq files exist (-: Aligning files matching /storage/SLY68/hic/juicer/lean1/fastq/*_R*.fastq* to genome hg19 with site file /storage/SLY68/hic/juicer/lean1/restriction_sites/gallus_MboI.txt (-: Created /storage/SLY68/hic/juicer/lean1/splits and /storage/SLY68/hic/juicer/lean1/aligned. /home/SLY68/biosoft/scripts/common/countligations.sh: command substitution: line 33: syntax error near unexpected token `(' /home/SLY68/biosoft/scripts/common/countligations.sh: command substitution: line 33: `paste <(gunzip -c $name1$ext) <(gunzip -c $name2$ext) | awk '!((NR+2)%4)' | grep -cE $ligation)' bwa mem -SP5M -t 144 /storage/SLY68/hic/juicer/lean1/references/Gallus_gallus.GRCg6a.dna.toplevel.fa /storage/SLY68/hic/juicer/lean1/splits/AF124701_R1.fastq.gz /storage/SLY68/hic/juicer/lean1/splits/AF124701_R2.fastq.gz > /storage/SLY68/hic/juicer/lean1/splits/AF124701.fastq.gz.sam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 10431158 sequences (1440000055 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19087, 3330422, 14076, 17770) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 2134, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14914) [M::mem_pestat] mean and std.dev: (3076.67, 2943.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (256.38, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1508, 3624, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16085) [M::mem_pestat] mean and std.dev: (4060.91, 2879.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 2632, 5648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15580) [M::mem_pestat] mean and std.dev: (3398.16, 2947.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10431158 reads in 3759.567 CPU sec, 107.906 real sec [M::process] read 10427450 sequences (1440000277 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19619, 3314616, 14195, 17953) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 2092, 5194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14964) [M::mem_pestat] mean and std.dev: (3044.15, 2943.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (257.74, 88.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3571, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (4025.09, 2864.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 2634, 5622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15606) [M::mem_pestat] mean and std.dev: (3372.26, 2946.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10427450 reads in 4253.256 CPU sec, 46.128 real sec [M::process] read 10422706 sequences (1440000122 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19888, 3306516, 14210, 17990) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 2072, 5226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15088) [M::mem_pestat] mean and std.dev: (3047.24, 2969.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.46, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3624, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16013) [M::mem_pestat] mean and std.dev: (4050.48, 2876.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (586, 2507, 5508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15352) [M::mem_pestat] mean and std.dev: (3319.27, 2962.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10422706 reads in 3971.485 CPU sec, 44.354 real sec [M::process] read 10421146 sequences (1440000194 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19881, 3308627, 14226, 17696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 2106, 5117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14747) [M::mem_pestat] mean and std.dev: (3017.17, 2923.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.46, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3572, 6311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (4028.30, 2885.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (563, 2491, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15644) [M::mem_pestat] mean and std.dev: (3315.13, 2979.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10421146 reads in 4350.945 CPU sec, 43.339 real sec [M::process] read 10419878 sequences (1440000180 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20037, 3303177, 14089, 18228) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 2099, 5264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15208) [M::mem_pestat] mean and std.dev: (3049.86, 2963.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.84, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3570, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15803) [M::mem_pestat] mean and std.dev: (4040.10, 2872.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 2447, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15427) [M::mem_pestat] mean and std.dev: (3278.65, 2947.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419878 reads in 3907.522 CPU sec, 48.270 real sec [M::process] read 10421234 sequences (1440000024 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20115, 3305652, 14305, 18147) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1999, 5096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14744) [M::mem_pestat] mean and std.dev: (2988.04, 2950.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.79, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3528, 6258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (4001.91, 2873.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 2477, 5604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (3302.04, 2971.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10421234 reads in 4353.929 CPU sec, 33.104 real sec [M::process] read 10420014 sequences (1440000152 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20204, 3305392, 14393, 18281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 2003, 5150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14934) [M::mem_pestat] mean and std.dev: (2996.44, 2969.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.79, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1488, 3520, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16053) [M::mem_pestat] mean and std.dev: (4008.44, 2888.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 2403, 5477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15409) [M::mem_pestat] mean and std.dev: (3250.09, 2954.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420014 reads in 3953.451 CPU sec, 39.974 real sec [M::process] read 10420086 sequences (1440000027 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20488, 3300282, 14187, 18483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1880, 4991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14483) [M::mem_pestat] mean and std.dev: (2909.43, 2926.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.15, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1488, 3537, 6356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16092) [M::mem_pestat] mean and std.dev: (4023.04, 2883.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (518, 2491, 5533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15563) [M::mem_pestat] mean and std.dev: (3284.88, 2962.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420086 reads in 4250.816 CPU sec, 33.185 real sec [M::process] read 10419694 sequences (1440000171 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20624, 3302510, 14226, 18415) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 1892, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14834) [M::mem_pestat] mean and std.dev: (2937.39, 2963.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.68, 88.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1485, 3525, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16014) [M::mem_pestat] mean and std.dev: (4013.49, 2874.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 2373, 5453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15415) [M::mem_pestat] mean and std.dev: (3218.26, 2948.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419694 reads in 3977.868 CPU sec, 39.536 real sec [M::process] read 10416184 sequences (1440000013 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20644, 3301715, 14262, 18928) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 1922, 5118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14874) [M::mem_pestat] mean and std.dev: (2953.42, 2968.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.61, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3518, 6285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15915) [M::mem_pestat] mean and std.dev: (3989.97, 2864.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (476, 2403, 5504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15560) [M::mem_pestat] mean and std.dev: (3238.13, 2966.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416184 reads in 4344.387 CPU sec, 33.027 real sec [M::process] read 10416210 sequences (1440000203 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20584, 3304563, 14279, 18815) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 1893, 5102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14820) [M::mem_pestat] mean and std.dev: (2959.79, 2981.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.47, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1490, 3556, 6306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (4016.44, 2872.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 2334, 5372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15276) [M::mem_pestat] mean and std.dev: (3183.41, 2944.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416210 reads in 3905.598 CPU sec, 30.560 real sec [M::process] read 10420782 sequences (1440000237 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20240, 3311290, 14297, 18650) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1930, 5094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14764) [M::mem_pestat] mean and std.dev: (2952.58, 2945.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.26, 88.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3636, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (4052.59, 2872.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 2330, 5431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15429) [M::mem_pestat] mean and std.dev: (3201.60, 2964.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420782 reads in 4308.940 CPU sec, 33.649 real sec [M::process] read 10429886 sequences (1440000101 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19195, 3318852, 13981, 17685) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 2229, 5360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15340) [M::mem_pestat] mean and std.dev: (3139.61, 2964.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (257.25, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1550, 3625, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16034) [M::mem_pestat] mean and std.dev: (4061.63, 2879.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 2576, 5641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15581) [M::mem_pestat] mean and std.dev: (3380.25, 2949.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10429886 reads in 3944.482 CPU sec, 33.902 real sec [M::process] read 10425588 sequences (1440000231 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19714, 3308449, 14077, 17913) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 2180, 5302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15234) [M::mem_pestat] mean and std.dev: (3098.26, 2957.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.22, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 3489, 6245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15757) [M::mem_pestat] mean and std.dev: (3980.98, 2866.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (609, 2584, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (3336.08, 2939.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10425588 reads in 4485.383 CPU sec, 41.801 real sec [M::process] read 10424096 sequences (1440000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19736, 3305532, 14218, 17923) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 2071, 5185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14957) [M::mem_pestat] mean and std.dev: (3023.54, 2941.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.63, 88.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3620, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (4067.83, 2868.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 2548, 5553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15519) [M::mem_pestat] mean and std.dev: (3326.40, 2961.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10424096 reads in 3983.351 CPU sec, 33.139 real sec [M::process] read 10419960 sequences (1440000145 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19659, 3300128, 14141, 18189) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 2117, 5220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15052) [M::mem_pestat] mean and std.dev: (3067.67, 2966.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.90, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3450, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (3963.99, 2859.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (577, 2511, 5548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (3318.38, 2968.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419960 reads in 4448.003 CPU sec, 33.917 real sec [M::process] read 10420846 sequences (1440000079 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19969, 3299891, 14246, 18276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 2050, 5192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15002) [M::mem_pestat] mean and std.dev: (3026.84, 2957.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.09, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3540, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15917) [M::mem_pestat] mean and std.dev: (4015.22, 2879.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (574, 2564, 5617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15703) [M::mem_pestat] mean and std.dev: (3339.36, 2963.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420846 reads in 3987.071 CPU sec, 34.186 real sec [M::process] read 10420770 sequences (1440000251 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20275, 3303420, 13792, 18276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2027, 5208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15080) [M::mem_pestat] mean and std.dev: (3011.08, 2959.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.70, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3556, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15896) [M::mem_pestat] mean and std.dev: (4014.54, 2884.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (534, 2498, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15330) [M::mem_pestat] mean and std.dev: (3278.77, 2938.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420770 reads in 4430.111 CPU sec, 33.498 real sec [M::process] read 10419954 sequences (1440000045 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20239, 3300506, 14213, 18333) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1999, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14777) [M::mem_pestat] mean and std.dev: (3002.73, 2960.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.05, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1481, 3559, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15953) [M::mem_pestat] mean and std.dev: (4025.15, 2871.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 2486, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15567) [M::mem_pestat] mean and std.dev: (3281.55, 2959.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419954 reads in 3950.315 CPU sec, 32.856 real sec [M::process] read 10418892 sequences (1440000244 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20460, 3300117, 14147, 18559) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1918, 5154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14972) [M::mem_pestat] mean and std.dev: (2963.50, 2982.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.09, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3557, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15826) [M::mem_pestat] mean and std.dev: (4007.87, 2852.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (485, 2357, 5414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (3230.52, 2940.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418892 reads in 4406.806 CPU sec, 34.524 real sec [M::process] read 10417270 sequences (1440000119 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20346, 3302135, 14198, 18812) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 1919, 5054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14652) [M::mem_pestat] mean and std.dev: (2949.75, 2953.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.79, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3620, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (4039.20, 2866.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (439, 2347, 5438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15436) [M::mem_pestat] mean and std.dev: (3209.17, 2957.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10417270 reads in 3946.773 CPU sec, 33.086 real sec [M::process] read 10415600 sequences (1440000090 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20467, 3300788, 14509, 18751) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 1855, 4976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14454) [M::mem_pestat] mean and std.dev: (2887.81, 2921.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.73, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3543, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16159) [M::mem_pestat] mean and std.dev: (4021.91, 2887.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (443, 2365, 5387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15275) [M::mem_pestat] mean and std.dev: (3200.91, 2945.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415600 reads in 4416.962 CPU sec, 34.191 real sec [M::process] read 10415956 sequences (1440000205 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20463, 3305399, 14171, 19117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 1938, 5058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14666) [M::mem_pestat] mean and std.dev: (2948.58, 2946.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.33, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3633, 6390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16122) [M::mem_pestat] mean and std.dev: (4060.51, 2880.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 2312, 5439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15523) [M::mem_pestat] mean and std.dev: (3194.67, 2981.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415956 reads in 3933.711 CPU sec, 32.163 real sec [M::process] read 10419204 sequences (1440000206 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20208, 3307803, 14113, 18769) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1924, 5128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14854) [M::mem_pestat] mean and std.dev: (2960.63, 2945.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.45, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3631, 6270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15802) [M::mem_pestat] mean and std.dev: (4036.54, 2865.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 2404, 5456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15462) [M::mem_pestat] mean and std.dev: (3235.79, 2968.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419204 reads in 4401.931 CPU sec, 33.627 real sec [M::process] read 10429486 sequences (1440000163 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19193, 3315005, 14040, 17803) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 2273, 5332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15266) [M::mem_pestat] mean and std.dev: (3138.32, 2953.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (257.54, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1480, 3507, 6324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (4010.15, 2891.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 2594, 5550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15286) [M::mem_pestat] mean and std.dev: (3370.19, 2925.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10429486 reads in 3950.217 CPU sec, 32.801 real sec [M::process] read 10425244 sequences (1440000063 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19266, 3307232, 14289, 17694) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 2151, 5279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3096.93, 2972.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.19, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1503, 3543, 6311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15927) [M::mem_pestat] mean and std.dev: (4017.49, 2866.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 2581, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15313) [M::mem_pestat] mean and std.dev: (3337.27, 2920.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10425244 reads in 4361.558 CPU sec, 34.208 real sec [M::process] read 10419998 sequences (1440000270 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19657, 3300100, 13912, 17844) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 2088, 5303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15293) [M::mem_pestat] mean and std.dev: (3066.53, 2963.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.71, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1475, 3602, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (4047.48, 2895.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 2616, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3348.26, 2921.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419998 reads in 3984.399 CPU sec, 33.023 real sec [M::process] read 10418050 sequences (1440000196 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19831, 3300998, 14249, 17886) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 2138, 5311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15285) [M::mem_pestat] mean and std.dev: (3092.59, 2965.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.88, 88.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1517, 3594, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15878) [M::mem_pestat] mean and std.dev: (4032.63, 2869.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (581, 2498, 5499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15335) [M::mem_pestat] mean and std.dev: (3296.95, 2939.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418050 reads in 4502.780 CPU sec, 34.296 real sec [M::process] read 10418540 sequences (1440000238 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19884, 3296336, 14352, 18038) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 2159, 5315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3102.15, 2979.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.99, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3603, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15927) [M::mem_pestat] mean and std.dev: (4026.60, 2874.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (566, 2478, 5573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15587) [M::mem_pestat] mean and std.dev: (3305.81, 2964.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418540 reads in 3962.100 CPU sec, 32.469 real sec [M::process] read 10416816 sequences (1440000253 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20013, 3297885, 14205, 17842) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 2131, 5240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15094) [M::mem_pestat] mean and std.dev: (3060.88, 2945.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.86, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1553, 3629, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16133) [M::mem_pestat] mean and std.dev: (4084.00, 2891.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 2528, 5562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15476) [M::mem_pestat] mean and std.dev: (3331.44, 2957.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416816 reads in 4398.084 CPU sec, 33.947 real sec [M::process] read 10416904 sequences (1440000065 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19932, 3298868, 14098, 18423) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 2076, 5183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14955) [M::mem_pestat] mean and std.dev: (3039.55, 2964.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.91, 88.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1506, 3518, 6169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15495) [M::mem_pestat] mean and std.dev: (3983.25, 2841.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (529, 2455, 5480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15382) [M::mem_pestat] mean and std.dev: (3265.88, 2946.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416904 reads in 4004.389 CPU sec, 33.520 real sec [M::process] read 10415758 sequences (1440000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20022, 3299530, 14385, 18370) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1947, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14663) [M::mem_pestat] mean and std.dev: (2962.80, 2933.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.86, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3599, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4044.34, 2893.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (487, 2427, 5482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3259.44, 2971.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415758 reads in 4469.774 CPU sec, 34.457 real sec [M::process] read 10416168 sequences (1440000239 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20418, 3297565, 14306, 18479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 2012, 5146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14938) [M::mem_pestat] mean and std.dev: (2979.91, 2945.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.98, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1468, 3570, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (4018.97, 2882.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (483, 2341, 5352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15090) [M::mem_pestat] mean and std.dev: (3211.88, 2949.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416168 reads in 3932.225 CPU sec, 33.962 real sec [M::process] read 10413702 sequences (1440000048 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20212, 3297317, 14415, 18928) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1931, 5117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14849) [M::mem_pestat] mean and std.dev: (2970.19, 2968.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.70, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3427, 6178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15628) [M::mem_pestat] mean and std.dev: (3946.69, 2860.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 2330, 5409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15321) [M::mem_pestat] mean and std.dev: (3195.72, 2952.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10413702 reads in 4467.531 CPU sec, 33.978 real sec [M::process] read 10413174 sequences (1440000043 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20639, 3302861, 14201, 18782) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1898, 5094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14788) [M::mem_pestat] mean and std.dev: (2941.53, 2960.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.53, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1529, 3592, 6297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15833) [M::mem_pestat] mean and std.dev: (4026.85, 2856.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 2389, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15257) [M::mem_pestat] mean and std.dev: (3202.04, 2942.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10413174 reads in 3941.840 CPU sec, 33.183 real sec [M::process] read 10420086 sequences (1440000005 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20135, 3306123, 14196, 18700) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1928, 5074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14700) [M::mem_pestat] mean and std.dev: (2951.53, 2950.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (258.31, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3555, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15884) [M::mem_pestat] mean and std.dev: (4018.38, 2871.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 2341, 5419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15303) [M::mem_pestat] mean and std.dev: (3204.72, 2940.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420086 reads in 4430.202 CPU sec, 34.507 real sec [M::process] read 10423098 sequences (1440000175 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19112, 3306845, 14137, 17803) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 2214, 5355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15339) [M::mem_pestat] mean and std.dev: (3121.24, 2958.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (258.16, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3601, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15918) [M::mem_pestat] mean and std.dev: (4027.11, 2872.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 2642, 5646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15578) [M::mem_pestat] mean and std.dev: (3386.00, 2945.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10423098 reads in 3965.803 CPU sec, 33.223 real sec [M::process] read 10418660 sequences (1440000197 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19266, 3299139, 14111, 17576) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 2143, 5268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15148) [M::mem_pestat] mean and std.dev: (3082.41, 2956.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.10, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3619, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15991) [M::mem_pestat] mean and std.dev: (4051.33, 2879.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 2536, 5570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (3319.79, 2929.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418660 reads in 4418.846 CPU sec, 34.274 real sec [M::process] read 10418866 sequences (1440000043 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19335, 3295977, 14422, 18184) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 2125, 5288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15232) [M::mem_pestat] mean and std.dev: (3083.96, 2973.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.25, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3619, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16202) [M::mem_pestat] mean and std.dev: (4074.14, 2895.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 2508, 5620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15630) [M::mem_pestat] mean and std.dev: (3336.82, 2947.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418866 reads in 3985.107 CPU sec, 32.397 real sec [M::process] read 10416366 sequences (1440000013 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19627, 3298606, 14404, 18127) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 2167, 5281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15167) [M::mem_pestat] mean and std.dev: (3085.04, 2938.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.00, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1529, 3535, 6279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (4023.11, 2855.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (568, 2538, 5574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15586) [M::mem_pestat] mean and std.dev: (3327.43, 2965.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416366 reads in 4437.490 CPU sec, 34.000 real sec [M::process] read 10416988 sequences (1440000254 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19576, 3296885, 14350, 18342) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 2131, 5348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3112.54, 2990.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.97, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3552, 6342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16042) [M::mem_pestat] mean and std.dev: (4019.97, 2887.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 2598, 5579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15529) [M::mem_pestat] mean and std.dev: (3347.09, 2946.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10416988 reads in 4028.808 CPU sec, 32.660 real sec [M::process] read 10415780 sequences (1440000062 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19983, 3298194, 14071, 18207) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 2126, 5249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15145) [M::mem_pestat] mean and std.dev: (3059.33, 2959.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.77, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3612, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16003) [M::mem_pestat] mean and std.dev: (4042.31, 2878.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (598, 2504, 5571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (3309.73, 2943.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415780 reads in 4412.999 CPU sec, 33.717 real sec [M::process] read 10414748 sequences (1440000046 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19881, 3298290, 14486, 18157) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 2095, 5238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15124) [M::mem_pestat] mean and std.dev: (3056.08, 2973.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.14, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3546, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15897) [M::mem_pestat] mean and std.dev: (4018.47, 2872.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (580, 2551, 5537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15451) [M::mem_pestat] mean and std.dev: (3311.20, 2941.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10414748 reads in 3877.324 CPU sec, 37.902 real sec [M::process] read 10415674 sequences (1440000036 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20041, 3295377, 14419, 18229) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 2054, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14733) [M::mem_pestat] mean and std.dev: (3007.78, 2931.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.95, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3609, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15816) [M::mem_pestat] mean and std.dev: (4040.42, 2864.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (537, 2432, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15123) [M::mem_pestat] mean and std.dev: (3235.95, 2918.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415674 reads in 4353.179 CPU sec, 33.351 real sec [M::process] read 10414652 sequences (1440000001 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20417, 3295554, 14166, 18439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 1929, 5020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14546) [M::mem_pestat] mean and std.dev: (2945.12, 2951.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.98, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3539, 6223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15659) [M::mem_pestat] mean and std.dev: (4001.65, 2861.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (505, 2429, 5428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15274) [M::mem_pestat] mean and std.dev: (3239.55, 2942.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10414652 reads in 3852.948 CPU sec, 33.809 real sec [M::process] read 10411218 sequences (1440000217 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20624, 3293831, 14279, 18399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 2012, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14778) [M::mem_pestat] mean and std.dev: (2983.70, 2941.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.10, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1483, 3508, 6324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4007.40, 2880.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 2408, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15684) [M::mem_pestat] mean and std.dev: (3260.66, 2970.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10411218 reads in 4337.135 CPU sec, 32.999 real sec [M::process] read 10410848 sequences (1440000207 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20189, 3296626, 14301, 18443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 1956, 5047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14635) [M::mem_pestat] mean and std.dev: (2949.09, 2947.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (258.78, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3589, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15856) [M::mem_pestat] mean and std.dev: (4025.19, 2874.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (449, 2358, 5425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15377) [M::mem_pestat] mean and std.dev: (3220.34, 2963.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10410848 reads in 3899.284 CPU sec, 39.320 real sec [M::process] read 10415194 sequences (1440000074 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20142, 3303234, 14352, 18817) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2008, 5156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14924) [M::mem_pestat] mean and std.dev: (3002.22, 2960.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (258.37, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1474, 3554, 6395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16237) [M::mem_pestat] mean and std.dev: (4035.94, 2890.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 2381, 5447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15423) [M::mem_pestat] mean and std.dev: (3221.73, 2955.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415194 reads in 4344.426 CPU sec, 33.804 real sec [M::process] read 10429514 sequences (1440000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19292, 3301905, 14286, 17891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 2285, 5360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15360) [M::mem_pestat] mean and std.dev: (3147.88, 2953.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (258.47, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1480, 3579, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16069) [M::mem_pestat] mean and std.dev: (4043.14, 2889.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 2658, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15593) [M::mem_pestat] mean and std.dev: (3389.11, 2937.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10429514 reads in 3936.418 CPU sec, 38.208 real sec [M::process] read 10420226 sequences (1440000260 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19489, 3292546, 14348, 17995) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 2213, 5341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15327) [M::mem_pestat] mean and std.dev: (3129.69, 2979.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.11, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3562, 6251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15699) [M::mem_pestat] mean and std.dev: (4012.54, 2847.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 2564, 5590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15506) [M::mem_pestat] mean and std.dev: (3348.26, 2946.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10420226 reads in 4399.564 CPU sec, 34.002 real sec [M::process] read 10421468 sequences (1440000136 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19656, 3293585, 14036, 17797) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 2115, 5242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15064) [M::mem_pestat] mean and std.dev: (3070.12, 2943.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.51, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3623, 6330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4044.52, 2862.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 2645, 5682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15772) [M::mem_pestat] mean and std.dev: (3393.01, 2955.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10421468 reads in 4001.714 CPU sec, 33.495 real sec [M::process] read 10419238 sequences (1440000101 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19624, 3293872, 14542, 17683) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 2176, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15420) [M::mem_pestat] mean and std.dev: (3106.42, 2980.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.45, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1503, 3527, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4023.90, 2881.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 2538, 5573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15473) [M::mem_pestat] mean and std.dev: (3338.44, 2948.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10419238 reads in 4389.245 CPU sec, 35.752 real sec [M::process] read 10417016 sequences (1440000025 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19497, 3289136, 14318, 18159) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 2147, 5240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15070) [M::mem_pestat] mean and std.dev: (3083.30, 2965.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (259.59, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3568, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4006.88, 2873.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 2614, 5616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15616) [M::mem_pestat] mean and std.dev: (3373.88, 2954.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10417016 reads in 3952.060 CPU sec, 32.928 real sec [M::process] read 10415212 sequences (1440000034 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19953, 3292374, 14099, 18146) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 2102, 5199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14995) [M::mem_pestat] mean and std.dev: (3048.49, 2971.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.11, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3519, 6250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15792) [M::mem_pestat] mean and std.dev: (3995.60, 2872.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 2621, 5607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15607) [M::mem_pestat] mean and std.dev: (3369.27, 2950.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415212 reads in 4314.165 CPU sec, 33.193 real sec [M::process] read 10414396 sequences (1440000159 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19559, 3294173, 14213, 17801) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 2053, 5243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15163) [M::mem_pestat] mean and std.dev: (3035.04, 2967.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (258.56, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3623, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (4066.04, 2868.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (587, 2558, 5599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15623) [M::mem_pestat] mean and std.dev: (3333.14, 2956.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10414396 reads in 3886.899 CPU sec, 38.640 real sec [M::process] read 10415296 sequences (1440000055 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19933, 3291652, 14449, 18216) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 2075, 5199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15023) [M::mem_pestat] mean and std.dev: (3035.40, 2969.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.28, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3593, 6366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16022) [M::mem_pestat] mean and std.dev: (4047.88, 2872.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (547, 2458, 5505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15421) [M::mem_pestat] mean and std.dev: (3271.29, 2952.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415296 reads in 4318.357 CPU sec, 33.073 real sec [M::process] read 10413778 sequences (1440000274 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20311, 3289192, 14141, 18618) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 2002, 5208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15082) [M::mem_pestat] mean and std.dev: (3024.57, 2985.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.12, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3479, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16148) [M::mem_pestat] mean and std.dev: (4007.57, 2888.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (499, 2421, 5491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15475) [M::mem_pestat] mean and std.dev: (3250.61, 2949.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10413778 reads in 3998.212 CPU sec, 31.034 real sec [M::process] read 10411942 sequences (1440000177 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20279, 3290556, 14152, 18682) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 1968, 5152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14942) [M::mem_pestat] mean and std.dev: (2962.04, 2947.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.16, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1501, 3601, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16027) [M::mem_pestat] mean and std.dev: (4036.53, 2882.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 2392, 5445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15421) [M::mem_pestat] mean and std.dev: (3221.12, 2957.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10411942 reads in 4238.379 CPU sec, 32.998 real sec [M::process] read 10411838 sequences (1440000101 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20520, 3291722, 13951, 18687) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 1947, 5044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14604) [M::mem_pestat] mean and std.dev: (2954.00, 2946.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.31, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1401, 3503, 6249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (3976.56, 2894.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (435, 2317, 5438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15444) [M::mem_pestat] mean and std.dev: (3202.34, 2977.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10411838 reads in 3973.408 CPU sec, 38.052 real sec [M::process] read 10418628 sequences (1440000064 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20099, 3301244, 14145, 18510) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 2061, 5172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14950) [M::mem_pestat] mean and std.dev: (3033.59, 2966.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.64, 88.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3538, 6338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16028) [M::mem_pestat] mean and std.dev: (4017.65, 2875.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 2380, 5393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15269) [M::mem_pestat] mean and std.dev: (3213.79, 2949.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10418628 reads in 4305.508 CPU sec, 39.514 real sec [M::process] read 10430558 sequences (1440000026 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18705, 3324613, 13847, 17667) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 2261, 5317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15199) [M::mem_pestat] mean and std.dev: (3145.68, 2962.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (256.62, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3584, 6264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15834) [M::mem_pestat] mean and std.dev: (4004.49, 2865.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 2710, 5687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15687) [M::mem_pestat] mean and std.dev: (3431.94, 2962.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10430558 reads in 3941.743 CPU sec, 41.744 real sec [M::process] read 10424508 sequences (1440000080 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19126, 3319243, 14326, 17673) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 2272, 5428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15514) [M::mem_pestat] mean and std.dev: (3169.96, 2964.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (257.45, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3524, 6310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15854) [M::mem_pestat] mean and std.dev: (4020.14, 2874.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 2611, 5618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15538) [M::mem_pestat] mean and std.dev: (3376.21, 2948.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10424508 reads in 4340.394 CPU sec, 34.141 real sec [M::process] read 10424466 sequences (1440000196 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19103, 3308988, 14288, 17548) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 2217, 5331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15245) [M::mem_pestat] mean and std.dev: (3126.47, 2958.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (258.06, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1499, 3555, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16115) [M::mem_pestat] mean and std.dev: (4041.90, 2885.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 2597, 5651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (3372.45, 2960.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10424466 reads in 3886.899 CPU sec, 34.042 real sec [M::process] read 10425048 sequences (1440000090 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18983, 3309125, 14160, 17945) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 2283, 5351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15289) [M::mem_pestat] mean and std.dev: (3149.07, 2960.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (258.24, 87.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1528, 3673, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15832) [M::mem_pestat] mean and std.dev: (4050.58, 2861.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 2658, 5611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15425) [M::mem_pestat] mean and std.dev: (3391.58, 2934.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10425048 reads in 4304.660 CPU sec, 33.422 real sec [M::process] read 10426396 sequences (1440000007 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19239, 3310165, 14184, 17847) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 2243, 5372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15378) [M::mem_pestat] mean and std.dev: (3143.89, 2965.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (258.23, 87.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3555, 6271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15723) [M::mem_pestat] mean and std.dev: (4012.12, 2853.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 2596, 5584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3344.56, 2924.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10426396 reads in 3945.948 CPU sec, 35.460 real sec [M::process] read 10423316 sequences (1440000250 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18950, 3307888, 14102, 17721) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 2242, 5301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15145) [M::mem_pestat] mean and std.dev: (3129.38, 2952.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.34, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3503, 6313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (4013.13, 2885.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 2643, 5641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15589) [M::mem_pestat] mean and std.dev: (3384.61, 2943.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10423316 reads in 4315.741 CPU sec, 33.161 real sec [M::process] read 10423350 sequences (1440000272 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19346, 3307335, 14236, 17780) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 2249, 5350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15306) [M::mem_pestat] mean and std.dev: (3139.59, 2956.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.45, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3585, 6280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15826) [M::mem_pestat] mean and std.dev: (4017.65, 2868.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 2595, 5576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15466) [M::mem_pestat] mean and std.dev: (3353.72, 2944.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10423350 reads in 3928.001 CPU sec, 37.281 real sec [M::process] read 10424358 sequences (1440000040 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19458, 3306593, 14340, 17832) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 2179, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15190) [M::mem_pestat] mean and std.dev: (3101.40, 2957.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.65, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3508, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (4006.57, 2889.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (577, 2526, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (3322.89, 2949.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10424358 reads in 4294.114 CPU sec, 32.913 real sec [M::process] read 10421734 sequences (1440000122 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19818, 3303100, 14424, 18218) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 2080, 5205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14995) [M::mem_pestat] mean and std.dev: (3045.04, 2943.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.82, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3501, 6208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15710) [M::mem_pestat] mean and std.dev: (3976.69, 2858.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (577, 2503, 5575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15571) [M::mem_pestat] mean and std.dev: (3320.04, 2967.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10421734 reads in 3936.331 CPU sec, 34.834 real sec [M::process] read 10417356 sequences (1440000169 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19601, 3303659, 14443, 18151) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 2029, 5191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14977) [M::mem_pestat] mean and std.dev: (3023.99, 2959.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.50, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3525, 6272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15830) [M::mem_pestat] mean and std.dev: (4012.27, 2869.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (559, 2479, 5516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15430) [M::mem_pestat] mean and std.dev: (3288.12, 2955.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10417356 reads in 4344.528 CPU sec, 33.494 real sec [M::process] read 10415860 sequences (1440000142 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19844, 3305178, 14192, 18430) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 2082, 5282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15238) [M::mem_pestat] mean and std.dev: (3065.99, 2972.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.53, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1495, 3596, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16093) [M::mem_pestat] mean and std.dev: (4044.97, 2881.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (549, 2530, 5542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15528) [M::mem_pestat] mean and std.dev: (3312.92, 2955.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10415860 reads in 3898.809 CPU sec, 31.991 real sec [M::process] read 10434164 sequences (1440000209 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19835, 3317733, 14129, 18258) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 2054, 5186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14954) [M::mem_pestat] mean and std.dev: (3021.43, 2936.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (257.72, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1481, 3530, 6228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15722) [M::mem_pestat] mean and std.dev: (3990.61, 2856.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (574, 2474, 5567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15553) [M::mem_pestat] mean and std.dev: (3310.46, 2968.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10434164 reads in 4288.446 CPU sec, 33.203 real sec [M::process] read 10469800 sequences (1440000256 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20766, 3322814, 14370, 18318) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1949, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14805) [M::mem_pestat] mean and std.dev: (2962.97, 2957.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 249, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (257.75, 87.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3602, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (4051.36, 2858.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (529, 2479, 5434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3261.32, 2927.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10469800 reads in 3968.001 CPU sec, 32.959 real sec [M::process] read 10467446 sequences (1440000252 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20738, 3310372, 14284, 18737) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 1923, 5136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14904) [M::mem_pestat] mean and std.dev: (2950.30, 2952.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.76, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3538, 6323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15931) [M::mem_pestat] mean and std.dev: (4030.01, 2881.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 2426, 5490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15450) [M::mem_pestat] mean and std.dev: (3245.94, 2940.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10467446 reads in 4207.451 CPU sec, 32.969 real sec [M::process] read 10460764 sequences (1440000274 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20936, 3308023, 14319, 18737) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1903, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (2949.12, 2957.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.14, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1481, 3534, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4007.39, 2880.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 2413, 5508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15610) [M::mem_pestat] mean and std.dev: (3243.06, 2964.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10460764 reads in 3960.036 CPU sec, 33.685 real sec [M::process] read 10460024 sequences (1440000258 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20866, 3305583, 14417, 18559) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 1814, 5014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14576) [M::mem_pestat] mean and std.dev: (2890.34, 2941.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.28, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3526, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15895) [M::mem_pestat] mean and std.dev: (3994.23, 2874.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 2439, 5454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15436) [M::mem_pestat] mean and std.dev: (3240.61, 2955.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10460024 reads in 4330.590 CPU sec, 33.683 real sec [M::process] read 10458520 sequences (1440000091 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20720, 3300494, 14184, 19167) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 1823, 4992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14490) [M::mem_pestat] mean and std.dev: (2897.15, 2939.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.61, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1511, 3585, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15977) [M::mem_pestat] mean and std.dev: (4041.43, 2870.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 2390, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15315) [M::mem_pestat] mean and std.dev: (3214.66, 2950.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458520 reads in 3957.108 CPU sec, 31.543 real sec [M::process] read 10457530 sequences (1440000133 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21230, 3303968, 14534, 19015) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 1883, 5035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14639) [M::mem_pestat] mean and std.dev: (2922.80, 2961.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.33, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3558, 6340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (4028.26, 2876.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 2342, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (3197.82, 2945.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10457530 reads in 4344.306 CPU sec, 33.686 real sec [M::process] read 10454298 sequences (1440000045 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21006, 3302127, 14601, 19094) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 1838, 5037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14649) [M::mem_pestat] mean and std.dev: (2916.25, 2961.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.29, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3601, 6314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (4034.61, 2876.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (422, 2330, 5538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15770) [M::mem_pestat] mean and std.dev: (3231.35, 2990.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10454298 reads in 3971.720 CPU sec, 38.763 real sec [M::process] read 10454136 sequences (1440000199 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21215, 3301011, 14423, 19092) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1774, 5052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14710) [M::mem_pestat] mean and std.dev: (2892.09, 2985.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.29, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3544, 6289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15795) [M::mem_pestat] mean and std.dev: (4022.62, 2869.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 2308, 5361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15257) [M::mem_pestat] mean and std.dev: (3173.07, 2957.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10454136 reads in 4407.376 CPU sec, 34.137 real sec [M::process] read 10455692 sequences (1440000056 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21306, 3305700, 14369, 19117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1805, 4915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14303) [M::mem_pestat] mean and std.dev: (2870.87, 2945.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.19, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3578, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15964) [M::mem_pestat] mean and std.dev: (4022.88, 2856.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (369, 2268, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15243) [M::mem_pestat] mean and std.dev: (3147.27, 2959.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10455692 reads in 3983.822 CPU sec, 40.528 real sec [M::process] read 10452036 sequences (1440000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21100, 3304267, 14340, 19399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1824, 5042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14670) [M::mem_pestat] mean and std.dev: (2913.57, 2972.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.17, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3614, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (4045.79, 2866.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (395, 2308, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (3189.90, 2977.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10452036 reads in 4203.347 CPU sec, 32.420 real sec [M::process] read 10450892 sequences (1440000037 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21144, 3305242, 14208, 19225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 1840, 5054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14702) [M::mem_pestat] mean and std.dev: (2917.12, 2972.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.06, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1490, 3649, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16310) [M::mem_pestat] mean and std.dev: (4070.57, 2892.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 2233, 5365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3146.48, 2971.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450892 reads in 4143.100 CPU sec, 32.552 real sec [M::process] read 10468790 sequences (1440000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20651, 3319820, 14209, 18298) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1961, 5139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14887) [M::mem_pestat] mean and std.dev: (2978.21, 2966.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (258.18, 87.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1425, 3554, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16077) [M::mem_pestat] mean and std.dev: (3998.79, 2892.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 2429, 5440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3248.58, 2948.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10468790 reads in 4275.213 CPU sec, 32.660 real sec [M::process] read 10466090 sequences (1440000192 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20482, 3307692, 14483, 18652) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1934, 5098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14800) [M::mem_pestat] mean and std.dev: (2959.11, 2975.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.16, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3585, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15992) [M::mem_pestat] mean and std.dev: (4056.70, 2872.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (520, 2472, 5427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15241) [M::mem_pestat] mean and std.dev: (3250.77, 2927.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10466090 reads in 4167.398 CPU sec, 32.224 real sec [M::process] read 10462478 sequences (1440000257 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20947, 3299723, 14461, 18751) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 1853, 5078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14746) [M::mem_pestat] mean and std.dev: (2933.83, 2972.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.78, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3542, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16097) [M::mem_pestat] mean and std.dev: (4023.99, 2902.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20968) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (497, 2475, 5545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15641) [M::mem_pestat] mean and std.dev: (3297.25, 2979.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10462478 reads in 4107.402 CPU sec, 31.518 real sec [M::process] read 10459692 sequences (1440000182 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20928, 3294197, 14357, 18661) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 1902, 5027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14597) [M::mem_pestat] mean and std.dev: (2931.87, 2958.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.30, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1500, 3508, 6310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15930) [M::mem_pestat] mean and std.dev: (3999.48, 2872.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 2485, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (3295.78, 2978.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10459692 reads in 4388.073 CPU sec, 33.643 real sec [M::process] read 10458550 sequences (1440000167 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21374, 3293073, 14495, 18964) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 1801, 4950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14376) [M::mem_pestat] mean and std.dev: (2891.47, 2961.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.32, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1511, 3516, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4029.36, 2874.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 2382, 5385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15241) [M::mem_pestat] mean and std.dev: (3205.12, 2939.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458550 reads in 3942.617 CPU sec, 37.982 real sec [M::process] read 10458018 sequences (1440000080 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21270, 3295703, 14383, 19061) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 1808, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14456) [M::mem_pestat] mean and std.dev: (2896.90, 2956.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.05, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3624, 6362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16098) [M::mem_pestat] mean and std.dev: (4043.37, 2873.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 2344, 5366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15218) [M::mem_pestat] mean and std.dev: (3192.93, 2939.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458018 reads in 4355.093 CPU sec, 33.363 real sec [M::process] read 10454588 sequences (1440000147 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21661, 3293960, 14473, 19088) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 1811, 4971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14437) [M::mem_pestat] mean and std.dev: (2880.77, 2947.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.19, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3565, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15927) [M::mem_pestat] mean and std.dev: (4046.34, 2863.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 2277, 5387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15343) [M::mem_pestat] mean and std.dev: (3171.53, 2955.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10454588 reads in 3958.709 CPU sec, 32.386 real sec [M::process] read 10453776 sequences (1440000080 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21135, 3290024, 14529, 19214) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 1854, 5006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14558) [M::mem_pestat] mean and std.dev: (2900.24, 2954.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.28, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1474, 3628, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16069) [M::mem_pestat] mean and std.dev: (4048.49, 2885.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 2253, 5407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15463) [M::mem_pestat] mean and std.dev: (3164.30, 2974.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453776 reads in 4431.536 CPU sec, 34.219 real sec [M::process] read 10453332 sequences (1440000248 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21355, 3297730, 14593, 19450) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 1740, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14476) [M::mem_pestat] mean and std.dev: (2854.29, 2951.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.66, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3503, 6262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15754) [M::mem_pestat] mean and std.dev: (4008.42, 2871.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 2232, 5337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15289) [M::mem_pestat] mean and std.dev: (3148.31, 2975.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453332 reads in 3947.832 CPU sec, 32.556 real sec [M::process] read 10450050 sequences (1440000019 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21450, 3299726, 14513, 19520) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 1701, 4921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14331) [M::mem_pestat] mean and std.dev: (2837.08, 2957.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.64, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3620, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4054.85, 2867.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 2219, 5338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15302) [M::mem_pestat] mean and std.dev: (3131.03, 2966.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450050 reads in 4426.939 CPU sec, 33.787 real sec [M::process] read 10471436 sequences (1440000083 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21370, 3307613, 14242, 19556) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 1756, 4886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14214) [M::mem_pestat] mean and std.dev: (2850.88, 2949.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.16, 88.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3599, 6324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15976) [M::mem_pestat] mean and std.dev: (4035.27, 2879.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 2203, 5305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15235) [M::mem_pestat] mean and std.dev: (3098.16, 2946.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10471436 reads in 3981.547 CPU sec, 33.919 real sec [M::process] read 10457152 sequences (1440000178 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20936, 3302940, 14657, 18933) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 1867, 5012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14554) [M::mem_pestat] mean and std.dev: (2918.37, 2956.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.36, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1541, 3598, 6349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15965) [M::mem_pestat] mean and std.dev: (4046.51, 2873.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 2314, 5454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15520) [M::mem_pestat] mean and std.dev: (3203.48, 2966.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10457152 reads in 4310.539 CPU sec, 33.227 real sec [M::process] read 10458872 sequences (1440000124 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20061, 3306899, 13995, 18297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 1986, 5153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14911) [M::mem_pestat] mean and std.dev: (2990.89, 2946.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.95, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3592, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4039.42, 2883.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (560, 2482, 5531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15473) [M::mem_pestat] mean and std.dev: (3292.50, 2943.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458872 reads in 4026.501 CPU sec, 33.523 real sec [M::process] read 10458202 sequences (1440000224 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20329, 3294264, 14622, 18444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 1965, 5167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14965) [M::mem_pestat] mean and std.dev: (2975.01, 2952.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.71, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3560, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15742) [M::mem_pestat] mean and std.dev: (4000.83, 2867.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 2434, 5456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15292) [M::mem_pestat] mean and std.dev: (3258.59, 2937.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458202 reads in 4386.708 CPU sec, 40.158 real sec [M::process] read 10453400 sequences (1440000009 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20873, 3291744, 14545, 18497) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 2006, 5119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14829) [M::mem_pestat] mean and std.dev: (2978.09, 2954.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.27, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1462, 3484, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15877) [M::mem_pestat] mean and std.dev: (3979.08, 2866.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (539, 2479, 5535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15527) [M::mem_pestat] mean and std.dev: (3292.49, 2951.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453400 reads in 3944.598 CPU sec, 34.772 real sec [M::process] read 10453366 sequences (1440000155 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20515, 3293703, 14468, 18727) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1854, 5040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14626) [M::mem_pestat] mean and std.dev: (2925.70, 2961.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.18, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3548, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15983) [M::mem_pestat] mean and std.dev: (4010.26, 2872.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (489, 2408, 5534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (3260.83, 2974.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453366 reads in 4356.651 CPU sec, 33.617 real sec [M::process] read 10453748 sequences (1440000117 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20915, 3289579, 14610, 18627) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 1867, 5001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14499) [M::mem_pestat] mean and std.dev: (2906.81, 2924.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (260.47, 88.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3564, 6248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (4012.80, 2857.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 2385, 5459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15469) [M::mem_pestat] mean and std.dev: (3230.73, 2966.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453748 reads in 3975.311 CPU sec, 32.863 real sec [M::process] read 10454090 sequences (1440000096 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21072, 3289190, 14582, 19036) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 1892, 5115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14849) [M::mem_pestat] mean and std.dev: (2933.01, 2959.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (260.49, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1454, 3530, 6211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15725) [M::mem_pestat] mean and std.dev: (3970.36, 2859.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (465, 2396, 5488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15534) [M::mem_pestat] mean and std.dev: (3243.98, 2958.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10454090 reads in 4390.435 CPU sec, 34.881 real sec [M::process] read 10450146 sequences (1440000101 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21000, 3290949, 14540, 18883) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 1814, 5046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14670) [M::mem_pestat] mean and std.dev: (2904.18, 2966.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.19, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1512, 3641, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4063.56, 2888.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 2307, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15204) [M::mem_pestat] mean and std.dev: (3173.18, 2935.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450146 reads in 3935.117 CPU sec, 36.528 real sec [M::process] read 10450732 sequences (1440000093 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21383, 3289610, 14485, 19398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1898, 5091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14801) [M::mem_pestat] mean and std.dev: (2947.05, 2978.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.13, 88.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3607, 6350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15990) [M::mem_pestat] mean and std.dev: (4056.44, 2868.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 2248, 5335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15211) [M::mem_pestat] mean and std.dev: (3154.48, 2953.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450732 reads in 4394.736 CPU sec, 33.245 real sec [M::process] read 10450514 sequences (1440000092 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21529, 3285857, 14536, 19538) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 1727, 4997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14547) [M::mem_pestat] mean and std.dev: (2874.58, 2975.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.25, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3415, 6180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15608) [M::mem_pestat] mean and std.dev: (3953.86, 2862.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 2247, 5324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15204) [M::mem_pestat] mean and std.dev: (3132.27, 2944.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450514 reads in 3947.732 CPU sec, 32.542 real sec [M::process] read 10447604 sequences (1440000276 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21304, 3293506, 14322, 19317) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 1733, 4933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14359) [M::mem_pestat] mean and std.dev: (2846.42, 2940.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.78, 88.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3568, 6315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (4026.03, 2883.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 2199, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15162) [M::mem_pestat] mean and std.dev: (3091.17, 2934.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10447604 reads in 4428.947 CPU sec, 33.741 real sec [M::process] read 10448542 sequences (1440000118 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21431, 3293864, 14491, 19242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1785, 4919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14301) [M::mem_pestat] mean and std.dev: (2862.55, 2929.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.88, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1495, 3551, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (4010.06, 2870.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 2194, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15156) [M::mem_pestat] mean and std.dev: (3096.34, 2949.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10448542 reads in 3960.861 CPU sec, 36.508 real sec [M::process] read 10467248 sequences (1440000119 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20542, 3302677, 14265, 18624) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1940, 5150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14932) [M::mem_pestat] mean and std.dev: (2979.94, 2969.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.42, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3675, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4055.42, 2870.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (489, 2394, 5534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (3257.35, 2978.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10467248 reads in 4365.770 CPU sec, 33.816 real sec [M::process] read 10459062 sequences (1440000266 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20586, 3291447, 14537, 18731) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1954, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14797) [M::mem_pestat] mean and std.dev: (2964.95, 2947.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.31, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1463, 3505, 6231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15767) [M::mem_pestat] mean and std.dev: (3973.61, 2865.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 2538, 5598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15706) [M::mem_pestat] mean and std.dev: (3318.53, 2966.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10459062 reads in 4005.425 CPU sec, 34.096 real sec [M::process] read 10455212 sequences (1440000149 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20635, 3281986, 14575, 18932) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1908, 5074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14722) [M::mem_pestat] mean and std.dev: (2945.67, 2958.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.73, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3629, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (4049.04, 2876.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 2335, 5413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15299) [M::mem_pestat] mean and std.dev: (3208.89, 2954.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10455212 reads in 4496.707 CPU sec, 34.209 real sec [M::process] read 10453290 sequences (1440000217 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20882, 3280895, 14462, 18675) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1896, 5081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14717) [M::mem_pestat] mean and std.dev: (2943.55, 2949.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (260.80, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3555, 6273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15825) [M::mem_pestat] mean and std.dev: (4006.68, 2870.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (478, 2392, 5412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15280) [M::mem_pestat] mean and std.dev: (3227.08, 2950.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453290 reads in 3971.327 CPU sec, 32.205 real sec [M::process] read 10453194 sequences (1440000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20677, 3287007, 14613, 18901) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 1906, 5097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14779) [M::mem_pestat] mean and std.dev: (2952.66, 2967.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.51, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1543, 3674, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16294) [M::mem_pestat] mean and std.dev: (4094.39, 2894.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 2423, 5492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15592) [M::mem_pestat] mean and std.dev: (3233.06, 2960.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453194 reads in 4346.022 CPU sec, 33.515 real sec [M::process] read 10452194 sequences (1440000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20884, 3283272, 14364, 18734) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1858, 5048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14644) [M::mem_pestat] mean and std.dev: (2912.72, 2940.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.70, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1496, 3559, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15887) [M::mem_pestat] mean and std.dev: (4018.97, 2882.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (485, 2352, 5420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3214.53, 2945.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10452194 reads in 3928.547 CPU sec, 34.182 real sec [M::process] read 10451580 sequences (1440000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21105, 3285880, 14370, 18893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 1866, 5129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14913) [M::mem_pestat] mean and std.dev: (2940.82, 2978.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.58, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1472, 3524, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15992) [M::mem_pestat] mean and std.dev: (4005.30, 2880.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 2420, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15607) [M::mem_pestat] mean and std.dev: (3248.27, 2976.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10451580 reads in 4296.612 CPU sec, 32.904 real sec [M::process] read 10450464 sequences (1440000163 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21156, 3285647, 14392, 19285) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 1770, 4905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14235) [M::mem_pestat] mean and std.dev: (2855.47, 2924.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.49, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1442, 3541, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (3994.30, 2878.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 2389, 5423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15361) [M::mem_pestat] mean and std.dev: (3224.39, 2965.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450464 reads in 3994.781 CPU sec, 33.007 real sec [M::process] read 10448216 sequences (1440000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21296, 3289265, 14322, 19106) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1815, 4989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14511) [M::mem_pestat] mean and std.dev: (2879.11, 2950.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.09, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3604, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4031.32, 2869.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 2323, 5423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15533) [M::mem_pestat] mean and std.dev: (3178.29, 2956.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10448216 reads in 4445.654 CPU sec, 34.126 real sec [M::process] read 10446400 sequences (1440000130 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21306, 3284853, 14246, 19102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1763, 5098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14848) [M::mem_pestat] mean and std.dev: (2887.93, 2973.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.30, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1501, 3534, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16015) [M::mem_pestat] mean and std.dev: (4023.95, 2884.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 2160, 5312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15258) [M::mem_pestat] mean and std.dev: (3100.03, 2964.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10446400 reads in 3965.175 CPU sec, 35.378 real sec [M::process] read 10448030 sequences (1440000234 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21441, 3291073, 14484, 19345) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 1769, 4957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14423) [M::mem_pestat] mean and std.dev: (2866.22, 2955.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.85, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3677, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16206) [M::mem_pestat] mean and std.dev: (4073.37, 2896.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 2195, 5321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15275) [M::mem_pestat] mean and std.dev: (3106.62, 2959.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10448030 reads in 4421.176 CPU sec, 33.298 real sec [M::process] read 10450562 sequences (1440000077 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20978, 3296319, 14309, 19223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1834, 4993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14507) [M::mem_pestat] mean and std.dev: (2892.14, 2944.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.64, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1445, 3517, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (3973.90, 2869.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 2283, 5410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15506) [M::mem_pestat] mean and std.dev: (3154.21, 2961.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10450562 reads in 3929.939 CPU sec, 34.483 real sec [M::process] read 10458232 sequences (1440000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20324, 3292992, 14277, 18299) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 1986, 5149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14901) [M::mem_pestat] mean and std.dev: (3002.03, 2967.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.84, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1482, 3587, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15972) [M::mem_pestat] mean and std.dev: (4024.71, 2878.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (531, 2500, 5565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (3307.33, 2966.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10458232 reads in 4433.901 CPU sec, 33.963 real sec [M::process] read 10454140 sequences (1440000173 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20798, 3285804, 14538, 18342) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 1967, 5109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14817) [M::mem_pestat] mean and std.dev: (2962.99, 2958.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.58, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3569, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (4007.89, 2858.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 2449, 5493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15391) [M::mem_pestat] mean and std.dev: (3265.70, 2938.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10454140 reads in 4074.356 CPU sec, 35.353 real sec [M::process] read 10456360 sequences (1440000194 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20660, 3284296, 14483, 18695) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 1943, 5111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14827) [M::mem_pestat] mean and std.dev: (2959.39, 2950.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.80, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1499, 3611, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16142) [M::mem_pestat] mean and std.dev: (4054.97, 2898.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (521, 2483, 5507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15479) [M::mem_pestat] mean and std.dev: (3271.49, 2947.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10456360 reads in 4435.502 CPU sec, 33.825 real sec [M::process] read 10455788 sequences (1440000023 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20699, 3283628, 14419, 18750) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 1938, 5121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14843) [M::mem_pestat] mean and std.dev: (2972.50, 2958.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.83, 89.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1491, 3507, 6236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (3992.46, 2866.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (497, 2491, 5522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (3287.74, 2974.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10455788 reads in 4036.956 CPU sec, 34.217 real sec [M::process] read 10453628 sequences (1440000237 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20369, 3280227, 14624, 18772) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 1943, 5131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14887) [M::mem_pestat] mean and std.dev: (2963.51, 2955.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.87, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3538, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15951) [M::mem_pestat] mean and std.dev: (4023.90, 2869.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 2375, 5450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15316) [M::mem_pestat] mean and std.dev: (3226.96, 2941.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10453628 reads in 4487.501 CPU sec, 34.235 real sec [M::process] read 10451644 sequences (1440000080 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21037, 3282291, 14419, 18786) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1897, 5080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14738) [M::mem_pestat] mean and std.dev: (2940.83, 2957.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.52, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3624, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15979) [M::mem_pestat] mean and std.dev: (4065.01, 2861.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (497, 2387, 5551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15659) [M::mem_pestat] mean and std.dev: (3268.32, 2981.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10451644 reads in 4044.715 CPU sec, 33.071 real sec [M::process] read 10449336 sequences (1440000222 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20948, 3284813, 14503, 18975) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1756, 4949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14375) [M::mem_pestat] mean and std.dev: (2868.25, 2956.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (259.95, 89.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3508, 6207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15607) [M::mem_pestat] mean and std.dev: (3983.65, 2848.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 2348, 5495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15585) [M::mem_pestat] mean and std.dev: (3222.22, 2981.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10449336 reads in 4455.405 CPU sec, 34.310 real sec [M::process] read 10447636 sequences (1440000009 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21530, 3281861, 14476, 19154) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 1851, 5005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14553) [M::mem_pestat] mean and std.dev: (2899.50, 2947.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.50, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1563, 3605, 6375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4071.11, 2888.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 2313, 5381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15317) [M::mem_pestat] mean and std.dev: (3179.62, 2942.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10447636 reads in 3968.790 CPU sec, 32.619 real sec [M::process] read 10445064 sequences (1440000070 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21136, 3281859, 14423, 19196) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1817, 5034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14660) [M::mem_pestat] mean and std.dev: (2906.40, 2981.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.08, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3586, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16061) [M::mem_pestat] mean and std.dev: (4046.74, 2887.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20919) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (364, 2234, 5376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15400) [M::mem_pestat] mean and std.dev: (3143.19, 2969.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10445064 reads in 4447.543 CPU sec, 38.773 real sec [M::process] read 10444876 sequences (1440000190 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21568, 3280692, 14556, 19602) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 1789, 4927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14333) [M::mem_pestat] mean and std.dev: (2862.55, 2942.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (260.38, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1500, 3569, 6300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4031.77, 2870.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (369, 2297, 5304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15174) [M::mem_pestat] mean and std.dev: (3150.38, 2955.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10444876 reads in 4017.600 CPU sec, 32.533 real sec [M::process] read 10445636 sequences (1440000184 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21145, 3283044, 14246, 19653) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 1702, 4953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14401) [M::mem_pestat] mean and std.dev: (2856.36, 2953.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (260.31, 89.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3565, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15870) [M::mem_pestat] mean and std.dev: (4033.37, 2871.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 2216, 5351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15323) [M::mem_pestat] mean and std.dev: (3137.40, 2957.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10445636 reads in 4407.633 CPU sec, 34.642 real sec [M::process] read 10462610 sequences (1440000154 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20702, 3303071, 14095, 18962) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 1937, 5073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14705) [M::mem_pestat] mean and std.dev: (2950.75, 2954.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.07, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3558, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16030) [M::mem_pestat] mean and std.dev: (4051.14, 2881.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 2429, 5462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15466) [M::mem_pestat] mean and std.dev: (3237.11, 2959.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10462610 reads in 3982.847 CPU sec, 32.849 real sec [M::process] read 10477374 sequences (1440000195 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20300, 3316424, 14742, 18172) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1983, 5083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14679) [M::mem_pestat] mean and std.dev: (2980.35, 2934.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (258.13, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1460, 3489, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (3993.94, 2883.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20828) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (582, 2537, 5525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15411) [M::mem_pestat] mean and std.dev: (3306.47, 2928.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10477374 reads in 4456.175 CPU sec, 36.608 real sec [M::process] read 10466434 sequences (1440000075 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20054, 3308603, 14456, 18163) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 1985, 5181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14965) [M::mem_pestat] mean and std.dev: (3012.89, 2969.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.75, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1486, 3510, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16069) [M::mem_pestat] mean and std.dev: (4014.06, 2883.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 2483, 5495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15381) [M::mem_pestat] mean and std.dev: (3292.36, 2949.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10466434 reads in 3954.570 CPU sec, 32.445 real sec [M::process] read 10466180 sequences (1440000196 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19976, 3308511, 14487, 18165) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 2057, 5181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14975) [M::mem_pestat] mean and std.dev: (3020.02, 2961.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.74, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3555, 6319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16017) [M::mem_pestat] mean and std.dev: (4011.89, 2874.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 2481, 5479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15333) [M::mem_pestat] mean and std.dev: (3275.97, 2934.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10466180 reads in 4418.158 CPU sec, 34.491 real sec [M::process] read 10464864 sequences (1440000161 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20086, 3307032, 14462, 18265) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 2084, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15385) [M::mem_pestat] mean and std.dev: (3067.41, 2973.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (259.00, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3637, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16019) [M::mem_pestat] mean and std.dev: (4065.08, 2882.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (573, 2550, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (3361.89, 2989.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10464864 reads in 3952.963 CPU sec, 32.285 real sec [M::process] read 10462920 sequences (1440000133 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19972, 3306949, 14311, 18161) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 2022, 5151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14873) [M::mem_pestat] mean and std.dev: (3016.83, 2942.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.11, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3546, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4001.45, 2877.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 2490, 5516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3294.85, 2944.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10462920 reads in 4499.456 CPU sec, 34.193 real sec [M::process] read 10459632 sequences (1440000071 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20250, 3306220, 14280, 18662) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 2105, 5260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15200) [M::mem_pestat] mean and std.dev: (3064.32, 2983.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.26, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3577, 6362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (4047.51, 2888.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 2531, 5509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15405) [M::mem_pestat] mean and std.dev: (3305.89, 2949.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10459632 reads in 3989.492 CPU sec, 34.726 real sec [M::process] read 10457964 sequences (1440000030 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20222, 3304824, 14539, 18348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 1957, 5065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14655) [M::mem_pestat] mean and std.dev: (2968.83, 2948.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (259.16, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1496, 3591, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (4013.01, 2872.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (496, 2527, 5540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15628) [M::mem_pestat] mean and std.dev: (3287.91, 2953.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10457964 reads in 4375.489 CPU sec, 33.885 real sec [M::process] read 10455584 sequences (1440000031 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20831, 3303087, 14453, 18555) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 2003, 5165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14959) [M::mem_pestat] mean and std.dev: (2991.76, 2944.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (259.05, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3484, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15724) [M::mem_pestat] mean and std.dev: (4003.34, 2869.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 2454, 5549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (3272.45, 2965.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10455584 reads in 3957.695 CPU sec, 32.334 real sec [M::process] read 10452426 sequences (1440000043 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20635, 3303892, 14238, 19112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 1923, 5054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14646) [M::mem_pestat] mean and std.dev: (2949.25, 2950.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (259.12, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3541, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15810) [M::mem_pestat] mean and std.dev: (3992.91, 2858.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (456, 2371, 5470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15498) [M::mem_pestat] mean and std.dev: (3232.36, 2970.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10452426 reads in 4323.843 CPU sec, 33.254 real sec [M::process] read 10451730 sequences (1440000205 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20488, 3304042, 14224, 19074) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1898, 5014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14540) [M::mem_pestat] mean and std.dev: (2926.12, 2936.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.86, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3579, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (4046.14, 2880.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 2292, 5438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15484) [M::mem_pestat] mean and std.dev: (3197.36, 2975.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 10451730 reads in 3941.696 CPU sec, 34.945 real sec [M::process] read 9745400 sequences (1342637281 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19083, 3081324, 13468, 17462) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 1978, 5133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14841) [M::mem_pestat] mean and std.dev: (2993.43, 2947.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (258.96, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3629, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4062.03, 2859.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (425, 2328, 5405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15365) [M::mem_pestat] mean and std.dev: (3195.15, 2952.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 9745400 reads in 3648.503 CPU sec, 28.255 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 144 /storage/SLY68/hic/juicer/lean1/references/Gallus_gallus.GRCg6a.dna.toplevel.fa /storage/SLY68/hic/juicer/lean1/splits/AF124701_R1.fastq.gz /storage/SLY68/hic/juicer/lean1/splits/AF124701_R2.fastq.gz [main] Real time: 6958.579 sec; CPU: 590693.370 sec (-: Align of /storage/SLY68/hic/juicer/lean1/splits/AF124701.fastq.gz.sam done successfully (-: Finished sorting all sorted files into a single merge. Picked up _JAVA_OPTIONS: -Xmx16384m sort: cannot create temporary file in ‘/storage/SLY68/hic/juicer/lean1/HIC_tmp’: No such file or directory Picked up _JAVA_OPTIONS: -Xmx16384m Not including fragment map Start preprocess Writing header Writing body ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................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Writing footer Finished preprocess Calculating norms for zoom BP_2500000 Calculating norms for zoom BP_1000000 Calculating norms for zoom BP_500000 Calculating norms for zoom BP_250000 Calculating norms for zoom BP_100000 Calculating norms for zoom BP_50000 Calculating norms for zoom BP_25000 Calculating norms for zoom BP_10000 Calculating norms for zoom BP_5000 Writing expected Writing norms Finished writing norms Picked up _JAVA_OPTIONS: -Xmx16384m Not including fragment map Start preprocess Writing header Writing body ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Writing footer Finished preprocess Calculating norms for zoom BP_2500000 Calculating norms for zoom BP_1000000 Calculating norms for zoom BP_500000 Calculating norms for zoom BP_250000 Calculating norms for zoom BP_100000 Calculating norms for zoom BP_50000 Calculating norms for zoom BP_25000 Calculating norms for zoom BP_10000 Calculating norms for zoom BP_5000 Writing expected Writing norms Finished writing norms /home/SLY68/biosoft/scripts/common/juicer_tools is post-processing Hi-C for hg19 Data read from /storage/SLY68/hic/juicer/lean1/aligned/inter_30.hic. Motifs read from /home/SLY68/biosoft/references/motif ARROWHEAD: Picked up _JAVA_OPTIONS: -Xmx16384m Reading file: /storage/SLY68/hic/juicer/lean1/aligned/inter_30.hic Default settings for 5kb being used max 208.0 0% 0% 1% 1% 2% 2% 3% 3% 4% 4% 5% 5% 6% 6% 7% 7% 8% 8% 9% 9% 10% 10% 11% 11% 12% 12% 12% 13% 13% 14% 14% 15% 15% 16% 16% 17% 17% 18% 18% 19% 19% 20% 20% 21% 21% 22% 22% 23% 23% 24% 24% 25% 25% 25% 26% 26% 27% 27% 28% 28% 29% 29% 30% 30% 31% 31% 32% 32% 33% 33% 34% 34% 35% 35% 36% 36% 37% 37% 37% 38% 38% 39% 39% 40% 40% 41% 41% 42% 42% 43% 43% 44% 44% 45% 45% 46% 46% 47% 47% 48% 48% 49% 49% 50% 50% 50% 51% 51% 52% 52% 53% 53% 54% 54% 55% 55% 56% 56% 57% 57% 58% 58% 59% 59% 60% 60% 61% 61% 62% 62% 62% 63% 63% 64% 64% 65% 65% 66% 66% 67% 67% 68% 68% 69% 69% 70% 70% 71% 71% 72% 72% 73% 73% 74% 74% 75% 75% 75% 76% 76% 77% 77% 78% 78% 79% 79% 80% 80% 81% 81% 82% 82% 83% 83% 84% 84% 85% 85% 86% 86% 87% 87% 87% 88% 88% 89% 89% 90% 90% 91% 91% 92% 92% 93% 93% 94% 94% 95% 95% 96% 96% 97% 97% 98% 98% 99% 99% 100% 89 domains written to file: /storage/SLY68/hic/juicer/lean1/aligned/inter_30_contact_domains/5000_blocks.bedpe Arrowhead complete HiCCUPS: GPUs are not installed so HiCCUPs cannot be run (-: Postprocessing successfully completed, maps too sparse to annotate or GPUs unavailable (-: ***! Error! The statistics do not add up. Alignment likely failed to complete on one or more files. Run relaunch_prep.sh Stats don't add up. Check /storage/SLY68/hic/juicer/lean1/aligned for results