Electroporation Wet Lab Style

[KD Derr]

Electroporation Wet Lab Style

Three Pieces to the Puzzle
1. Power Supply- Model 400
2. Invitrogen Electroporator
3. Oscilloscope

Power Supply Set Up
Volts- 500v -modulating the voltage dial will result in faster or slower charing of the Electroporator
Watts- 20 Watts -dial 12 o’clock
Amps- 50mAmps -dial 3 o’clock

Make sure that the wiring diagram and parameters are set correctly
Turn Power On
DC -Push start button. Hold briefly. If it does not stay on something is not set up properly

Electroporation
Charge
Flip switch to CHARGE
Load resistance- 500 Ohms
Capacitance- 660v
Armed- to pulse
When charge light stays on (see notes if light does not go solid within 1 minute)
Verify the safety circuit is closed
Set trigger on Oscilloscope
Flip switch to PULSE

Notes- When charging light is blinking the battery is charging. When light stays on it is fully charged. If you do not trigger immediately when fully charged. Charge will bleed off and will not fully charge without discharge. This can be regulated by speeding up or slowing down the charge with the voltage dial on the power supply.

GwInstek Oscilloscope GDS-820C
1. Trigger -Push {Menu}
    Set TYPE to PULSE -Use {F1} to change
    Set SOURCE to CHANNEL BEING USED -Use {F2} to change
    Set MODE to SINGLE -Use {F3} to change
2. Set {CH1} to 200mV using Yellow Volts/Div Knob
3. Set TRIGGER Level Knob to 200mV
4. Set HORIZONTAL TIME/DIV knob to 50mS
5. Push RUN/STOP to RESET TRIGGER