NEPA21 electroporator

[Taku Tokuyasu]

Just for reference, some links to the NEPA21 electroporator from Nepagene before I forget.
My Web searches from a year ago suggested this is currently the best device for transforming Chlamydomonas.  It is essentially a square wave generator as I understand, but they have some special sauce on shaping and duration.  It is not cheap.  But perhaps we can get some hints from the literature on it.

http://www.nepagene.jp/index2.html
- Possibly the US/UK distributor is Sonidel: http://www.sonidel.com/
  http://www.sonidel.com/sonidel/electroporation/nepa21-electroporator/

 (Sonidel also has a sonoporator:  http://www.sonidel.com/sonidel/sonidel-sp100-sonoporator/;
  Sonoporation-optimized plasmids: http://sonidel.com/Pdf/Brochure%20-%20STK10.pdf
 )

Another distributor of the NEPA21 (I think Wallace Marshall lab at UCSF uses this)
Bulldog Bio, Inc.
URL: www.bulldog-bio.com
Bulldog Bio provides quality products for life science research. On display will be the NEPA21 Electro-Kinetic Transfection System that has been proven to be THE most efficient way to deliver nucleic acids into Chlamydomonas. Check out why the NEPA21 is Japan's most popular electroporator.
- From http://www.genetics-gsa.org/chlamydomonas/2014/pdf/Program%20Book%20final.pdf