Journal Club - 29 March 2016

[Kyle Bates]

We will meet at Lestat's on Park Ave at 7pm.  We may move one block north to Twiggs if Lestat's is too crowded.

Christian's notes and links on his Journal Club topic this Tuesday:

Here are a few papers; I had a very hard time narrowing it down to these papers, there is just too many fascinating things about P Putida.  The first 4 papers I suggest you read, the rest are articles of interest, but aren’t part of the discussion.  We will be focusing primarily on P Putida’s ability to induce calcite precipitation and is applications in heavy metal pollution sequestration.  

A few notes I’m working with Pseudomonas Putida to remove copper (Which is meant to simulate lead). P Putida has a different method of inducing calcite precipitation, than the bacteria in many of the articles we will be reading, which use a urease reaction.  The main differences is that P Putida doesn’t create ammonia byproducts.  However since the formation of calcite is extracellular the mode of heavy metal sequestration should be the same.

https://microbewiki.kenyon.edu/index.php/Pseudomonas_putida
A quick and light read that gives a good idea of the bacteria, and highlights many of its applications.

http://springerplus.springeropen.com/articles/10.1186/s40064-016-1869-2
A good review of the method of heavy metal sequestration by use of calcite precipitation.  Note that in the article it says calcite formed by Bacillus sp using calcium acetate is metastable, this is not the case for P Putida as evidenced by the 4th article. I recommend reading the article in full, but definitely read the MICP applications and Mechanism of calcite precipitation sections.

http://moscow.sci-hub.io/778558c4680e76dbca9f30f378404ff4/achal2011.pdf
A study using Kocuria flava CR1 to induce calcite precipitation and sequester copper.

http://www.scielo.br/scielo.php?pid=S1517-83822011000200014&script=sci_arttext
A article that contains an investigation in to the calcite formation of P Putida. It unfortunately lacks a theory to P Putida method of calcite formation.  But contains evidence that strongly suggests that calcite formed by P Putida is very stable and doesn’t form ammonia byproducts.

Other articles, that may be of interest.

http://link.springer.com/article/10.1007/s00253-012-3928-0
Industrial biotechnology of Pseudomonas putida and related species
Highlights many of the products that can be produced by P Putida

https://etd.ohiolink.edu/rws_etd/document/get/ohiou1156790187/inline
Reduction of TNT by P Putida
Disarming land mines

http://link.springer.com/article/10.1007/s002530050683
Cell-free extract(s) of Pseudomonas putida catalyzes the conversion of cyanides, cyanates, thiocyanates, formamide, and cyanide-containing mine waters into ammonia

http://www.ncbi.nlm.nih.gov/pubmed/14641576
Characterization of a new solvent-responsive gene locus in Pseudomonas putida F1 and its functionalization as a versatile biosensor.

http://www.sciencedirect.com/science/article/pii/S1389172303801767
Isolation and application of a styrene-degrading strain of Pseudomonas putida to Biofiltration

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300010
Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852 (edited)

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