Hello,
The format for the RNK file is two columns separated by a tab. The first column is the gene/protein/etc and the second is the ranking metric. Below is a short example:
FBXL16 5.090173777154632
CAB39L 5.082362201539112
GPRC5C 4.3249735944185606
RHOB 4.243918178282676
I also attached a full rnk file.
You can usually copy-paste from excel into webgestalt if you are using the website (
webgestalt.org). There are also examples that you can view.
1. Select GSEA
2. Load example
3. Example RNK file.
Let me know if you have any questions or further issues.
Best,
John