ERROR: The number of annotated IDs for all functional categories are not from 3 to 2000 for the GSEA enrichment method.

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leandro villalobos

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Nov 17, 2024, 9:59:33 PM11/17/24
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Hi everyone. I am having a problem trying to run a GSEA for my non model organism. 

I am having this error message:

ERROR: The number of annotated IDs for all functional categories are not from 3 to 2000 for the GSEA enrichment method.

Even after I set my database with at least 3 genes for geneset.  

Someone knows what could be happening.

This is the data I am uploading.

Best,
Leandro


GSEA rank (NaBu).rnk
KEGG database.gmt

John Elizarraras

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Nov 18, 2024, 1:11:59 PM11/18/24
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Hello,

It seems that your GMT file is not in the correct format. There are extra spaces before each analyte which causes the error you see and there blank elements at the end of most gene lists. I fixed the GMT and attached it below.

Best,
John
KEGG database.gmt
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