custom analysis on WebGestaltR

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Joe South

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Feb 9, 2023, 2:13:31 PM2/9/23
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Hello

I am trying to perform an analysis using a non-model organism.  I have generated the files and ran a small test version of the files on the web version and it executes correctly (the entire set of files appears to be too large to run on the web). 

So I successfully installed it in R studio and use the same exact files as the ones I used for the web, and ran it using

WebGestaltR(enrichMethod="ORA", organism="others", enrichDatabaseFile = "/database-test.gmt",  enrichDatabaseDescriptionFile = "/description_test.des", interestGeneFile = "/genelist_test.txt", referenceGeneFile = "/reference.txt")

 but I get the following error.

Loading the functional categories...
Loading the ID list...
Loading the reference list...
Performing the enrichment analysis...
Error in oraEnrichment(interestGeneList, referenceGeneList, geneSet, minNum = minNum, :
 ERROR: No genes in the interesting list can annotate to any functional category.

Any assistance will be greatly welcomed.
Best Regards
Lu
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