ts_stack unexpectedly removes tilts

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Qiuye Li

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May 19, 2026, 1:29:21 PMMay 19
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Dear all,
While I'm trying to find the best alignment, I realized that many tilt series in tiltstack/ are incomplete, with some even down to 2 tilts. It happens both when using wrapper as well as using ts_stack separately.
I can confirm all my *.tomostar have the complete tilt list.
I also rerun ts_import with --min_intensity 0, but it didn't change anything.
Any ideas or help will be greatly appreciated.

Best,
Qiuye

Qiuye Li

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May 20, 2026, 11:13:52 AMMay 20
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I think I've figured this out. For those of us who are still wondering:
It looks like re-running ts_import does not overwrite the XML files. I removed the previous result and re-ran ts_import without --min_intensity, now all tilts are included in stacks now.

Best,
Qiuye

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