Tomogram deconvolution in Warp

[Mingyu Jung]

Hi all,

I have a quick question regarding tomogram deconvolution in Warp (Windows).

I was wondering what algorithm is used for tomogram deconvolution in Warp.
Specifically, is it a Wiener-type CTF deconvolution, or is it limited to phase flipping only?

Thank you very much for your help.

Best,
Mingyu

[Ricardo Righetto]

Hi Mingyu,

It is a Wiener-type CTF deconvolution as described in the original Warp paper, Supplementary Figure 2:

https://www.nature.com/articles/s41592-019-0580-y/figures/8

The intuition is to boost the very low frequencies to enhance contrast, while attenuating everything past the first zero crossing of the CTF to remove noise.

Best wishes,

--
Ricardo Diogo Righetto

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[Mingyu Jung]

Dear Ricardo

Thank you for your help. It really helps.

Best,
Mingyu

2026년 1월 27일 화요일 AM 12시 50분 46초 UTC-8에 ricardo...@gmail.com님이 작성: