Re: Tomograms CTF corrected? - Warp 1.1

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Alister Burt

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May 21, 2025, 11:01:11 AM5/21/25
to Arjun Bhatta, Warp
Hi Arjun,

The simple answer is yes, tomograms are CTF corrected. For more details please see the warp paper :-)



Cheers,

Alister

Sent from mobile - apologies for brevity

On May 21, 2025, at 07:48, Arjun Bhatta <arjun.bh...@gmail.com> wrote:


Hi all,
Just briefly wanted to ask: are the tomograms reconstructed using "Reconstruct tomogram" interface by Warp v1.1 CTF corrected already? My understanding is that the subtomograms are not CTF corrected but the CTF, tilt and accumulated dose information is instead in the 3D-CTF files that are written out along with the subtomograms. But, I am not clear on the how the tomograms are treated; especially because I read some answers on the forum saying the tomograms are CTF corrected (which I also assumed), but I saw that Warp 1.1 also writes out 3D-CTF files for the tomograms in reconstruction/ctf directory, and I am wondering why they are necessary (something with denoising?) if the tomograms are already CTF corrected.
Best,
Arjun

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Alister Burt

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May 21, 2025, 11:02:11 AM5/21/25
to Arjun Bhatta, Warp
I’m not sure what those extra files were intended for but denoising seems likely
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